| 1. |
- Karan, Kunal, et al.
(författare)
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Implications of future climate change on crop and irrigation water requirements in a semi-arid river basin using CMIP6 GCMs
- 2022
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Ingår i: Journal of Arid Land. - : Springer Science and Business Media LLC. - 1674-6767 .- 2194-7783. ; 14:11, s. 1234-1257
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Tidskriftsartikel (refereegranskat)abstract
- Agriculture faces risks due to increasing stress from climate change, particularly in semi-arid regions. Lack of understanding of crop water requirement (CWR) and irrigation water requirement (IWR) in a changing climate may result in crop failure and socioeconomic problems that can become detrimental to agriculture-based economies in emerging nations worldwide. Previous research in CWR and IWR has largely focused on large river basins and scenarios from the Coupled Model Intercomparison Project Phase 3 (CMIP3) and Coupled Model Intercomparison Project Phase 5 (CMIP5) to account for the impacts of climate change on crops. Smaller basins, however, are more susceptible to regional climate change, with more significant impacts on crops. This study estimates CWRs and IWRs for five crops (sugarcane, wheat, cotton, sorghum, and soybean) in the Pravara River Basin (area of 6537 km2) of India using outputs from the most recent Coupled Model Intercomparison Project Phase 6 (CMIP6) General Circulation Models (GCMs) under Shared Socio-economic Pathway (SSP)245 and SSP585 scenarios. An increase in mean annual rainfall is projected under both scenarios in the 2050s and 2080s using ten selected CMIP6 GCMs. CWRs for all crops may decline in almost all of the CMIP6 GCMs in the 2050s and 2080s (with the exceptions of ACCESS-CM-2 and ACCESS-ESM-1.5) under SSP245 and SSP585 scenarios. The availability of increasing soil moisture in the root zone due to increasing rainfall and a decrease in the projected maximum temperature may be responsible for this decline in CWR. Similarly, except for soybean and cotton, the projected IWRs for all other three crops under SSP245 and SSP585 scenarios show a decrease or a small increase in the 2050s and 2080s in most CMIP6 GCMs. These findings are important for agricultural researchers and water resource managers to implement long-term crop planning techniques and to reduce the negative impacts of climate change and associated rainfall variability to avert crop failure and agricultural losses.
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| 2. |
- Knyazeva, Anastasia, 1995-, et al.
(författare)
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A chemical inhibitor of IST1-CHMP1B interaction impairs endosomal recycling and induces noncanonical LC3 lipidation
- 2024
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 121:17
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Tidskriftsartikel (refereegranskat)abstract
- The endosomal sorting complex required for transport (ESCRT) machinery constitutes multisubunit protein complexes that play an essential role in membrane remodeling and trafficking. ESCRTs regulate a wide array of cellular processes, including cytokinetic abscission, cargo sorting into multivesicular bodies (MVBs), membrane repair, and autophagy. Given the versatile functionality of ESCRTs, and the intricate organizational structure of the ESCRT machinery, the targeted modulation of distinct ESCRT complexes is considerably challenging. This study presents a pseudonatural product targeting IST1-CHMP1B within the ESCRT-III complexes. The compound specifically disrupts the interaction between IST1 and CHMP1B, thereby inhibiting the formation of IST1-CHMP1B copolymers essential for normal-topology membrane scission events. While the compound has no impact on cytokinesis, MVB sorting, or biogenesis of extracellular vesicles, it rapidly inhibits transferrin receptor recycling in cells, resulting in the accumulation of transferrin in stalled sorting endosomes. Stalled endosomes become decorated by lipidated LC3, suggesting a link between noncanonical LC3 lipidation and inhibition of the IST1-CHMP1B complex.
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| 3. |
- Paulsson, Magnus, et al.
(författare)
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Peptidoglycan-Binding Anchor Is a Pseudomonas aeruginosa OmpA Family Lipoprotein With Importance for Outer Membrane Vesicles, Biofilms, and the Periplasmic Shape
- 2021
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Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- The outer membrane protein A (OmpA) family contains an evolutionary conserved domain that links the outer membrane in Gram-negative bacteria to the semi-rigid peptidoglycan (PG) layer. The clinically significant pathogen Pseudomonas aeruginosa carries several OmpA family proteins (OprF, OprL, PA0833, and PA1048) that share the PG-binding domain. These proteins are important for cell morphology, membrane stability, and biofilm and outer membrane vesicle (OMV) formation. In addition to other OmpAs, in silico analysis revealed that the putative outer membrane protein (OMP) with gene locus PA1041 is a lipoprotein with an OmpA domain and, hence, is a potential virulence factor. This study aimed to evaluate PA1041 as a PG-binding protein and describe its effect on the phenotype. Clinical strains were confirmed to contain the lipoprotein resulting from PA1041 expression with Western blot, and PG binding was verified in enzyme-linked immunosorbent assay (ELISA). By using a Sepharose bead-based ELISA, we found that the lipoprotein binds to meso-diaminopimelic acid (mDAP), an amino acid in the pentapeptide portion of PGs. The reference strain PAO1 and the corresponding transposon mutant PW2884 devoid of the lipoprotein were examined for phenotypic changes. Transmission electron microscopy revealed enlarged periplasm spaces near the cellular poles in the mutant. In addition, we observed an increased release of OMV, which could be confirmed by nanoparticle tracking analysis. Importantly, mutants without the lipoprotein produced a thick, but loose and unorganized, biofilm in flow cells. In conclusion, the lipoprotein from gene locus PA1041 tethers the outer membrane to the PG layer, and mutants are viable, but display severe phenotypic changes including disordered biofilm formation. Based upon the phenotype of the P. aeruginosa PW2884 mutant and the function of the protein, we designate the lipoprotein with locus tag PA1041 as “peptidoglycan-binding anchor” (Pba).
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| 4. |
- Söderholm, Niklas, et al.
(författare)
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Exploring the bacterial nano-universe
- 2020
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Ingår i: Current opinion in structural biology. - : Elsevier. - 0959-440X .- 1879-033X. ; 64, s. 166-173
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Forskningsöversikt (refereegranskat)abstract
- Since the days of the first acknowledged microscopist, Antonie van Leeuwenhoek, the 'animalcules', that is, bacteria and other microbes have been subject to increasingly detailed visualization. With the currently most sophisticated molecular imaging method; cryo electron tomography (Cryo-ET), we are reaching the milestone of being able to image an entire organism in a single dataset at nanometer resolution. Cryo-ET will enable the next revolution in our understanding of bacterial cells, their ultra-structure and intricate molecular nanomachines. Here, we highlight recent research discoveries based on constantly progressing technology developments. We discuss advantages and challenges of using Cryo-ET to visualize spatial structure of microorganisms and macromolecular complexes in their native environment.
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