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| 2. |
- Sundin, Johanna, et al.
(author)
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High yield direct 76Br-bromination of monoclonal antibodies using Cloramine-T
- 1999
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In: Nuclear Medicine and Biology. - 0969-8051 .- 1872-9614. ; 26:8, s. 923-929
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Journal article (peer-reviewed)abstract
- Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.
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| 3. |
- Zhao, Qinghai, et al.
(author)
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Effects of dextranation on the pharmacokinetics of short peptides : A PETstudy on mEGF
- 1999
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In: Bioconjugate chemistry. - : American Chemical Society (ACS). - 1043-1802 .- 1520-4812. ; 10:6, s. 938-946
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Journal article (peer-reviewed)abstract
- The effects of dextranation on the biodistribution of mouse epidermal growth factor (mEGF, 6 kDa) were assessed. By reductive amination, mEGF was coupled to 13 and 46 kDa dextran. The two dextranated conjugates and free mEGF were labeled with the positron-emitting nuclide (76)Br (T(1/2) = 16 h). After intravenous administration to Sprague Dawley rats, the radioactivity biodistribution was evaluated by positron emission tomography (PET) and by measurements of dissected tissues. The dextranation prolonged the retention time in blood, especially when the dextran chain was long. [(76)Br]mEGF-dextran conjugates were shown to have significantly, more than 5 times, lower kidney accumulation than the nonconjugated [(76)Br]mEGF. In conclusion, dextranation affects the biodistribution of mEGF in vivo giving a prolonged circulation time, a decreased uptake in kidney, and an increased spleen accumulation.
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| 4. |
- Bergström, Mats, et al.
(author)
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PET with [11C]-Metomidate for the Visualization of Adrenocortical Tumors and Discrimination from Other Lesions
- 1999
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In: Clinical Positron Imaging. - 1095-0397 .- 1878-5751. ; 2:6, s. 339-
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Journal article (peer-reviewed)abstract
- Purpose:The purpose of the study was to evaluate the potential role of PET with the adrenocortical-specific tracer 11C-metomidate in the characterization of incidentally found adrenal cortical lesions and in adrenocortical carcinomas.Methods:PET with 11C-metomidate was performed in 15 patients with unilateral adrenal mass confirmed by CT (incidentalomas) and in 9 additional patients with adrenocortical cancer. All incidentalomas subsequently underwent surgery, except 2 subjected to biopsy only. These lesions were histopathologically examined and diagnosed as adrenal cortical adenoma (n = 6; 3 nonfunctioning), adrenocortical carcinoma (n = 2) and nodular hyperplasia (n = 1). The remaining were non-cortical lesions including 1 pheochromocytoma, 1 myelolipoma, 2 adrenal cysts, and 2 metastases.Results:All lesions, except 1, with an adrenocortical origin were easily identified due to exceedingly high uptake of 11C-metomidate, whereas the non-cortical lesions showed very low uptake. The 1 false negative was a cancer that at surgery was found to be extensively necrotic. High uptake was also seen in normal adrenal glands. The tracer uptake kinetics indicated trapping of the tracer in the cortical lesions. For quantitative evaluation of tracer binding in individual lesions, the simple SUV concept was found to be equally accurate as more elaborate kinetic analyses.Conclusion:The patients presented and altogether over 40 PET investigations have demonstrated 11C-metomidate to be an attractive tracer for the characterization of adrenal masses with the ability to discriminate lesions of adrenal cortical origin from non-cortical lesions. Additionally the method allows the assessment of metastases from adrenocortical cancers, and the very high contrast has allowed partial whole-body examinations.
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| 5. |
- Carlsson, Jörgen, et al.
(author)
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Conjugate chemistry and cellular processing of EGF-dextran
- 1999
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In: Acta Oncologica. - 0284-186X .- 1651-226X. ; 38:3, s. 313-321
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Journal article (peer-reviewed)abstract
- Conjugates with specific binding to the epidermal growth factor receptor, EGFR, of interest for radionuclide based imaging and therapy were prepared using mouse epidermal growth factor, mEGF, and dextran. In one type of conjugate, mEGF was coupled to dextran by reductive amination in which the free amino group on the mEGF N-terminal reacted with the aldehyde group on the reductive end of dextran. The end-end coupled conjugate could be further activated by the cyanopyridinium agent CDAP, thereby introducing tyrosines to the dextran part. In the other type of conjugate, the cyanylating procedure using CDAP was applied, first to activate dextran and then allowing for the amino terminus of mEGF to randomly attach to the dextran. In the latter case, radionuclide-labelled tyrosines or glycines could be added in the same conjugation step. All types of mEGF-dextran conjugates had EGFR-specific binding since the binding could be displaced by an excess of non-radioactive mEGF. The conjugates were to a large extent internalized in the test cells and the associated radioactivity was retained intracellularly for different times depending on both the type of cells and conjugate applied. Different intracellular 'traffic routes' for the radionuclides are discussed as well as applications for both imaging and therapy.
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| 6. |
- Edgren, Maliha, et al.
(author)
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[111In-DPTA-D-Phe1] - Octreotide Scintigraphy in the Management of Patients with Advanced Renal Cell Carcinoma
- 1999
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In: Cancer Biotherapy and Radiopharmaceuticals. - : Mary Ann Liebert Inc. - 1084-9785 .- 1557-8852. ; 14:1, s. 59-64
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Journal article (other academic/artistic)abstract
- Somatostatin receptor scintigraphy using the 111In-labelled somatostatin analogue octreotide (Octreoscan) was performed in 9 patients with metastatic renal cell carcinoma. In total 11 scintigraphies were performed. Positive tumor uptakes were observed in 9 patients. The results of the octreotide scans were correlated to diagnostic CT and/or X-ray images. Forty (59%) out of 68 known tumor localizations were visualized with the octreotide scan. A second scan following therapy was performed in two patients. These patients showed progressive disease despite treatment and also exhibited intensified uptakes at octreotide scintigraphy. One false positive lesion was observed in the 40 lesions visualized in scintigraphy.It was concluded that renal cell carcinoma expresses somatostatin receptors, as could be visualized with Octreoscan scintigraphy. The scintigraphic technique can be used as an instrument for in vivo characterization of the disease. The data could also form a basis for future investigations regarding the possible therapeutic effect of octreotide in the management of renal cell cancer.
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| 7. |
- Eriksson, Barbro, et al.
(author)
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Positron emission tomography in neuroendocrine tumours
- 1999
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In: The Italian Journal of Gastroenterology and Hepatology. - 1125-8055. ; Suppl 2, s. S167-S171
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Journal article (peer-reviewed)abstract
- Positron emission tomography is an in vivo tracer and imaging technique that utilizes short-lived positron emitting radionuclides (11C, 15O, 13N, 18F) with half-lives ranging between 2 min and 2 hours. These radionuclides are interesting from the labelling viewpoint since they are natural constituents of most biologically active compounds. The short half-life is an advantage with regard to the irradiation dose to the patient but it is also a limitation since it requires the production of these radionuclides in close vicinity to the positron emission tomography camera.
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| 8. |
- Hanson, Lars, et al.
(author)
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Annie, a Tool for Integrating Ergonomics in the Design of Car Interiors
- 1999
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In: Journal of Materials & Manufacturing. ; 108:5, s. 1114-1124
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Journal article (peer-reviewed)abstract
- In this ANNIE project – Applications of Neural Networks to Integrated Ergonomics – BE96-3433, a tool for integrating ergonomics into the design process is developed. This paper presents some features in the current ANNIE as applied to the design of car interiors. A variant of the ERGOMan mannequin with vision is controlled by a hybrid system for neuro-fuzzy simulation. It is trained by using an Elite system for registration of movements. An example of a trajectory generated by the system is shown. A fuzzy model is used for comfort evaluation. An experiment was performed to test its feasibility and it showed very promising results.
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| 9. |
- Hanson, Lars, et al.
(author)
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ANNIE, a Tool for Integrating Ergonomics in the Design of Car Interiors
- 1999
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In: SAE technical paper series. - 400 Commonwealth Drive, Warrendale, PA, United States : SAE International. - 0148-7191. ; 108:5, s. 1114-1124
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Journal article (peer-reviewed)abstract
- In the ANNIE project - Applications of Neural Networks to Integrated Ergonomics - BE96-3433, a tool for integrating ergonomics into the design process is developed. This paper presents some features in the current ANNIE as applied to the design of car interiors. A variant of the ERGOMan mannequin with vision is controlled by a hybrid system for neuro-fuzzy simulation. It is trained by using an Elite system for registration of movements. An example of a trajectory generated by the system is shown. A fuzzy model is used for comfort evaluation. An experiment was performed to test its feasibility and it showed very promising results.
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| 10. |
- Hansson, Lars, et al.
(author)
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Annie, a Tool for Integrating Ergonomics in the Design of Car Interiors
- 1999
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Conference paper (other academic/artistic)abstract
- An example of a result from a long-term cooperation with Lund University (together with professor Roland Axelsson at the Department for Work Environment) there some of the authors. (Engström) gained extensive grants (Wallenberg Stifelsen regarding equipment as well as other founding from e.g. the Swedish Work Environment Found).
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