| 1. |
- Beconyte, G., et al.
(författare)
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Atlas of the Baltic Sea Region (ABSR)
- 2008
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Ingår i: 7th International Conference on Environmental Engineering, ICEE 2008. - : Vilnius Gediminas Technical University Publishing House Technika. - 9789955282563 ; , s. 1261-1268
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Konferensbidrag (refereegranskat)abstract
- The Baltic Sea Region (BSR) has long been important in many aspects. The BSR is the first region in the world that has adopted common regional goals fos sutainable development. It is estimated that there will be a considerable increase in the number of regional geographic information strategies being implemented through the world in the next ten years. It will naturally raise interest in synthetic geographic information on the BSR, as on one of the most developed regions in the world. Atlas of the Baltic Sea Region (ABSR) has been planned as a complex and consistent cartographic edition with a purpose to represent the region as a whole in the best way possible. The goal of the paper is to introduce with a different user's groups and their specific needs. General difficulties and strengths (high level SWOT analysis) are described. The contents of the Atlas are designed as a modular structure that allows combining units of the Atlas in the most convenient way. It has been sought for most comprehensive territorial generalization of the accumulated scientific knowledge. The structure and particular maps are planned in a way to integrate natural and socio-cultural aspects as well as history and geography of the region, to demonstrate internal territorial links and provide a macro-regional context. Description of implementation focuses on the breakdown of project activities and tasks that illustrates the entire framework of ABSR including time schedule. Resources (hardware, software, information, knowledge) and responsibilities of team members are assigned to each task. Modern approach to design and management of the informational system for the Atlas and modern cartographic technologies were chosen to assure efficiency of works.
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| 2. |
- Efimenko, Evgeni, et al.
(författare)
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Analysis of xbx genes in C-elegans
- 2005
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Ingår i: Development. - : The Company of Biologists. - 0950-1991 .- 1477-9129. ; 132:8, s. 1923-1934
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Tidskriftsartikel (refereegranskat)abstract
- Cilia and flagella are widespread eukaryotic subcellular components that are conserved from green algae to mammals. In different organisms they function in cell motility, movement of extracellular fluids and sensory reception. While the function and structural description of cilia and flagella are well established, there are many questions that remain unanswered. In particular, very little is known about the developmental mechanisms by which cilia are generated and shaped and how their components are assembled into functional machineries. To find genes involved in cilia development we used as a search tool a promoter motif, the X-box, which participates in the regulation of certain ciliary genes in the nematode Caenorhabditis elegans. By using a genome search approach for X-box promoter motif-containing genes (xbx genes) we identified a list of about 750 xbx genes (candidates). This list comprises some already known ciliary genes as well as new genes, many of which we hypothesize to be important for cilium structure and function. We derived a C elegans X-box consensus sequence by in vivo expression analysis. We found that xbx gene expression patterns were dependent on particular X-box nucleotide compositions and the distance from the respective gene start. We propose a model where DAF-19, the RFX-type transcription factor binding to the X-box, is responsible for the development of a ciliary module in C elegans, which includes genes for cilium structure, transport machinery, receptors and other factors.
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| 3. |
- Petersson, Ulrika A., et al.
(författare)
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The Prx Q protein of Arabidopsis thaliana is a member of the luminal chloroplast proteome
- 2006
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Ingår i: FEBS Letters. - : Wiley. - 0014-5793 .- 1873-3468. ; 580:26, s. 6055-6061
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Tidskriftsartikel (refereegranskat)abstract
- Peroxiredoxins have been discovered in many organisms ranging from eubacteria to mammals, and their known biological functions include both oxidant defense and signal transduction. The genome of Arabidopsis thaliana encodes for ten individual peroxiredoxins, of which four are located in the chloroplast. The best-characterized member of the chloroplast peroxiredoxins is 2-Cys Prx that is associated with the stroma side of the thylakoid membrane and is considered to participate in antioxidant defense and protection of photosynthesis. This study addressed the chloroplast peroxiredoxin Prx Q and showed that its subcellular location is the lumen of the thylakoid membrane. To get insight in the biological function of the Prx Q protein of Arabidopsis, the protein levels of the Prx Q protein in thylakoid membranes were studied under different light conditions and oxidative stress. A T-DNA knockout mutant of Prx Q did not show any visible phenotype and had normal photosynthetic performance with a slightly increased oxygen evolving activity.
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| 4. |
- Rich, Rebecca L., et al.
(författare)
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A global benchmark study using affinity-based biosensors
- 2009
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 386:2, s. 194-216
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Tidskriftsartikel (refereegranskat)abstract
- To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
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