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Effects of interfer...
Effects of interferon alpha on the expression of p21cip1/waf1 and cellcycle distribution in carcinoid tumors.
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- Zhou, Y (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Wang, S (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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Yue, BG (författare)
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visa fler...
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- Gobl, A (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Oberg, K (författare)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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visa färre...
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(creator_code:org_t)
- 2002
- 2002
- Engelska.
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Ingår i: Cancer Invest. ; 20, s. 348-
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Interferon alpha (IFN-alpha) has been shown to produce antitumor effects in 50-80% of carcinoid tumor patients and has demonstrated anti-proliferative effects in carcinoid tumor cells, but the mechanism is not well established. This study presents evidence that in a carcinoid tumor cell line, Bon1, IFN-alpha increases the expression of p21 and promotes nuclear translocation of endogenous p21. Furthermore, immunoprecipitation experiments demonstrated that p21 formed immuno-complexes with Stat1 and Stat2 in the nucleus of cells. Interferon alpha can decrease G1- and G2-phase cells, but increase S-phase population. The p21 mRNA expression is inversely correlated to the G1 population (r = -0.933, P < 0.05) and positively correlated to the S-phase population (r = 0.901, P < 0.05). In addition, IFN-alpha inhibited cyclin dependent kinases (CDK), CDK2-, CDK3-, CDK4-, and cyclin E- but not cyclin A-associated kinase activities. Immunodepletion of p21 resulted in a significant enhancement of CDK3 kinase activity (approximately 1.6-fold increase). These results suggest that the mechanism of antitumor and cell cycle regulation of IFN-alpha in carcinoid tumors may, at least in part, be p21-dependent. Based on these results, we conclude that IFN-alpha exerts antitumor effects by increased p21 expression in neuroendocrine tumors.
Nyckelord
- interferon alpha
- gene expression
- p21Cip1/Waf1
- signal transduction
- carcinoid
- neuroendocrine tumor
- cell cycle
- cyclin dependent kinase
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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