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- Liu, Y-J, et al.
(författare)
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Glucose induces oscillations of cytoplasmic Ca2+, Sr2+ and Ba2+ in pancreatic beta-cells without participation of the thapsigargin-sensitive store
- 1995
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Ingår i: Cell Calcium. ; 18:2, s. 165-173
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Tidskriftsartikel (refereegranskat)abstract
- Individual pancreatic beta -cells were used to study the glucose effects on the handling of Ca2+, Sr2+ and Ba2+. In extracellular medium containing one of these ions, single beta -cells responded to 11 mM glucose with large amplitude oscillations in cytoplasmic Ca2+, Sr2+ or Ba2+ with indistinguishable average frequencies (0.30-0.33/min). The oscillations disappeared after hyperpolarization with 400 microM diazoxide. Under such hyperpolarization, glucose stimulated the sequestration of Ca2+ and Sr2+ but not of repetitively mobilized by consecutive exposures to 100 microM carbachol. A 2-3 min exposure to 100 nM of the intracellular Ca(2+)-ATPase inhibitor thapsigargin also mobilized Ca2+ and Sr2+ and irreversibly abolished subsequent release by carbachol. However, thapsigargin did not prevent the large amplitude oscillations in Ca2+, Sr2+ or Ba2+ under non-hyperpolarizing conditions although the frequency of the Ca2+ oscillations was almost doubled. The results indicate that the slow oscillatory behavior of glucose-stimulated individual beta -cells does not depend on inositol 1,4,5-trisphosphate mediated release of intracellular Ca2+.
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