| 1. |
|
|
| 2. |
- Fu, X, et al.
(author)
-
Oxytocin-induced oscillations of cytoplasmic Ca2+ in human myometrial cells.
- 2000
-
In: Acta Obstetricia et Gynecologica Scandinavica. - 0001-6349 .- 1600-0412. ; 79:3
-
Journal article (peer-reviewed)abstract
- BACKGROUND: To investigate the mechanisms of oxytocin (OT) induced oscillations of the cytoplasmic Ca2+ concentration ([Ca2+]i) in cultured human myometrial cells.METHODS: [Ca2+]i was measured in individual myometrial cells by dual wavelength spectrophotofluorometry using the fluorescent indicator fura-2. Myometrium was obtained at abdominal hysterectomy (n=8) and during cesarean section (n=7).RESULTS: OT (10-300 nM) typically induced [Ca2+]i oscillations with frequencies in the 0.6-0.8/min range. There were no obvious differences in the responses of cells taken from non-pregnant and term pregnant women. The frequency and amplitude of the oscillations were not significantly affected by OT concentrations up to 300 nM. The amplitude of the oscillations decreased in the presence of the voltage-dependent Ca2+ channel antagonist verapamil and gradually disappeared in Ca2+-free medium. The oscillations were further blocked by the inorganic Ca2+ antagonist La3+ and by the intracellular Ca2+-ATPase inhibitor 2.5-di-tert-butylhydroquinone (DTBHQ). Caffeine inhibited the OT-induced oscillations in a concentration-dependent manner. DTBHQ and high concentrations of OT made [Ca2+]i remarkably sensitive to changes in the external Ca2+ concentration.CONCLUSIONS: The results indicate that OT-induced [Ca2+]i oscillations in human myometrial cells are due to inositol 1,4,5-trisphosphate-mediated release of intracellular Ca2+ combined with capacitative as well as voltage-dependent influx of the ion.
|
|
| 3. |
- Liu, Y. Q., et al.
(author)
-
An improved model for soil ionization around grounding system and its application to stratified soil
- 2004
-
In: Journal of Electrostatics. - : Elsevier BV. - 0304-3886 .- 1873-5738. ; 60:2-4, s. 203-209
-
Journal article (peer-reviewed)abstract
- An improved model for taking into account the effect of the soil ionization around grounding system under lightning strike is proposed in this paper. In this model, the soil ionization region is assumed to retain 7% of its pre-ionization resistivity, which is consistent with the experimental results on soil ionization found in literature (Trans. SA Inst. Electr. Eng. (1988) 63; AIEE Trans. 61 (1942) 349; Proc. IEE 121(2) (1974) 123) and our own laboratory experiments (Time domain modelling of the response of grounding systems subjected to lightning currents, Licenciate Thesis, Uppsala University, 2003). Compared with modelling the soil ionization as an increase in the size of the ground conductor, the model presented here will not overestimate the beneficial influence of the soil ionization in reducing the ground potential rise, especially in high resistivity soil. The model is also applied to study the transient behaviour of grounding conductors in stratified soil under lightning strike including soil ionization. It shows that making the grounding conductor to penetrate the lower resistivity soil layer could help to decrease the ground potential rise at the injection point several times.
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
