| 1. |
- Humble, Elisabet, et al.
(författare)
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Amino acid sequence at the phosphorylated site of rat liver fructose-1,6-diphosphatase and phosphorylation of a corresponding synthetic peptide
- 1979
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 90:3, s. 1064-1072
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Tidskriftsartikel (refereegranskat)abstract
- Rat liver fructose-1,6-diphosphatase was phosphorylated with (32P)ATP and the catalytic subunit of cyclic AMP-dependent protein kinase from pig muscle. After digestion with pepsin, α-chymotrypsin and subtilisin a peptide with the amino-terminal sequence Ser-Arg-Tyr-(32P)SerP-Leu-Pro-Leu-Pro was isolated. A synthetic unphosphorylated heptapeptide with the same amino acid sequence, ending with leucine, was phosphorylated with an apparent Km of 400 μM, while the apparent Km value for fructose-1,6-diphosphatase was 30 μM (subunit concentration). The Vmax value was 20 times higher for the peptide than for the enzyme.
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| 2. |
- Belfrage, Per, et al.
(författare)
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Dispersion of viable pig liver cells with collagenase
- 1975
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Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
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Tidskriftsartikel (refereegranskat)abstract
- Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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| 3. |
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| 4. |
- Edvardsson, Bo, 1944-
(författare)
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Metodkatalog för "geriatrisk aktivering"
- 1976
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Rapport (populärvet., debatt m.m.)abstract
- This report is an account of 30 answers to a questionnaire to Swedish hospitals 1976 and some points of view from the author.
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| 5. |
- Hagander, Per, et al.
(författare)
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Models for the Insulin Response to Intravenous Glucose
- 1978
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Ingår i: Mathematical Biosciences. - : Elsevier BV. - 0025-5564. ; 42, s. 15-29
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Tidskriftsartikel (refereegranskat)abstract
- The Grodsky packet storage model describes many features of insulin release, but at present more or less arbitrary simplifications are necessary. The consequences of various simplifications are discussed, especially with regard to identification of parameters thought to be of importance for glucose tolerance. In particular, the insulin release dynamics of the ordinary intravenous glucose tolerance test is examined. The proposed model contains the following features: It considers arterial rather than venous blood glucose concentration as the stimulus, it takes the glucose injection time into account, and it contains a positive derivative term during the rise of the glucose concentration. When the insulin elimination-rate time constant is fixed to an a priori value, model fitting gives a clear quantification of the sensitivity of early and late insulin release to glucose.
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| 6. |
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| 7. |
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| 8. |
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| 9. |
- Axelsson, Inge
(författare)
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Biology of corneal and skeletal proteoglycans
- 1978
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Ingår i: Biochimie des tissus conjonctifs normaux et pathologiques : Biochemistry of normal and pathological connective tissues / 6th Colloquium of the Federation of European connective tissue clubs, Faculté de médecine, Université Paris Val-de Marne Créteil 28-30 août 1978; publ. sous la direction de A.-M. Robert et L. Robert. - 2222025222
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Konferensbidrag (refereegranskat)
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| 10. |
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