| 1. |
- Cabras, Tiziana, et al.
(författare)
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Carbachol-induced in vitro secretion of certain human submandibular proteins investigated by mass-spectrometry.
- 2008
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 53:11, s. 1077-83
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To investigate protein content of saliva produced in vitro by samples of human submandibular gland following stimulation with the muscarinic agent carbachol. DESIGN: Tissue samples, obtained at surgery from seven patients and showing normal morphological appearance, were tested for 30 min: in absence of carbachol and atropine; in presence of carbachol (10 microM); in presence of carbachol (10 microM) and atropine (20 microM); or in presence of just atropine (20 microM). Medium was analysed by high-performance liquid chromatography-mass-spectrometry. Neither before nor during surgery were the patients exposed to drug treatments that were likely to influence the in vitro secretion. RESULTS: Proline-rich proteins (PRP)-1 and -3, peptide PC and PB, statherin, cystatins SN, S1 and S2 were invariably found in control gland tissue medium. Mean concentrations of these proteins/peptides in the medium were non-proportionally elevated following carbachol exposure to the gland tissues. Difference between basal release and carbachol-induced secretion achieved statistical significance as to all the proteins/peptides under study but for statherin. Atropine alone or atropine plus carbachol caused no significant changes compared to the basal release of proteins/peptides. CONCLUSIONS: In vitro studies on salivary glands make it possible to study protein secretion from individual glands and thus, to reveal the contribution of the various types of gland to protein/peptide content of whole saliva. The disproportional responses to carbachol may imply that the proteins/peptides are not confined to the same cells or to the same intracellular locations and are therefore not secreted as packages at parasympathetic cholinergic activity.
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| 2. |
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| 3. |
- Darnell, Malin, et al.
(författare)
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Lipopolysaccharide induced-in vivo increases in beta-defensins of the rat parotid gland.
- 2006
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 51:9, s. 769-74
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Tidskriftsartikel (refereegranskat)abstract
- Antimicrobial beta-defensins are thought to protect epithelial surfaces. Their mobilization in response to inflammation was studied in the rat parotid gland using an ELISA assay. Bacterial lipopolysaccharide (LPS), injected into the parotid duct on one side, induced a marked local inflammatory response in the parotid gland as judged by several fold increases in myeloperoxidase activity and, in histological sections, infiltration of neutrophils. Three hours after the injection, beta-defensin 1 and 3 were increased (by 41% and 15%, respectively, P<0.01) as compared to the contralateral gland. Though still elevated 6h after the injection, the percentage figures for beta-defensin 1 were, at this time, somewhat lower (30%) compared to the situation at 3h, while those for defensin 3 were significantly higher 65% (P<0.01); neither at the early nor at the late time of observation were any changes in the level of beta-defensin 2 observed. The beta-defensins under study were not detected in submandibular and sublingual glands, neither were they detected in the inflamed submandibular gland, showing also here several fold increases in myeloperoxidase activity and, in addition, the presence of inflammatory cells, following ductal injection of LPS towards the gland.
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| 4. |
- Eliasson, Lars, 1940, et al.
(författare)
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Feeling of dry mouth in relation to whole and minor gland saliva secretion rate.
- 2009
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 54:3, s. 263-7
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Tidskriftsartikel (refereegranskat)abstract
- Previous reports indicate an association between whole saliva secretion rate and xerostomia, but the association is not always found in individuals complaining of dry mouth. In the present study, we hypothesised that the minor salivary gland secretions could affect subjective feelings of oral dryness in individuals with normal or low whole saliva secretion rate. 142 individuals, aged 18-82 years, participated in the study. Feelings of oral dryness were assessed separately for day- and nighttime by visual analogue scales. Resting and stimulated whole saliva flow rates were measured by conventional methods, and minor palatal, buccal and labial saliva secretion rates by the Periotron method. Twenty-three percent of the women and 15% of the men reported feelings of dry mouth during day and night. The results showed statistically significant lower resting and stimulated whole and minor labial gland saliva secretion rates in subjects reporting oral dryness during both day and night compared with non-complainers. In groups of individuals with normal simulated whole saliva secretion, those who complained from dry mouth both day and night had statistically significant lower labial secretion rate compared with those who had no complaints. Although not statistically significant, this difference was seen also in the groups with fewer individuals who had low whole saliva flow. In summary, our findings suggested that the labial gland saliva might affect subjective feelings of dry mouth both in individuals with normal and subnormal whole saliva flow.
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| 5. |
- Eliasson, Lars, 1940, et al.
(författare)
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Minor gland saliva flow rate and proteins in subjects with hyposalivation due to Sjogren's syndrome and radiation therapy.
- 2005
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 50:3, s. 293-9
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the secretion rate and IgA, albumin and lactoferrin concentrations in minor labial and buccal gland saliva were investigated in individuals with hyposalivation due to primary Sjogren's syndrome (pSS; 10 subjects) or head and neck radiation therapy (RT; 10 subjects) and in their matched controls. Whole saliva was similarly examined. The minor gland saliva flow was measured using the Periotron method. IgA, albumin and lactoferrin concentrations were analysed by ELISA techniques. A general finding was that the flow rate and protein concentrations were lower in labial than in buccal gland saliva. In both hyposalivation groups, the labial minor gland saliva secretion rate was lowered compared to their respective controls. The buccal gland saliva flow rate was significantly reduced in the RT group only. IgA and albumin concentrations were not different from the controls in the labial secretions. The concentration of lactoferrin was increased in the RT group. In buccal saliva, the concentrations of all proteins examined but pSS IgA, were increased compared to the controls. Reduced flow rate and increased protein concentrations were seen for whole saliva where the lactoferrin concentration was higher in RT than in pSS subjects. Thus, our findings suggested that minor gland saliva flow rate and protein concentrations are affected in RT and pSS subjects and to highest extent in the former.
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| 6. |
- Eriksson, Per-Olof, et al.
(författare)
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Jaw-neck dysfunction in whiplash-associated disorders
- 2007
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969 .- 1879-1506. ; 52:4, s. 404-408
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Tidskriftsartikel (refereegranskat)abstract
- This paper reports data from recent studies on integrative jaw-neck motor control in healthy subjects and disturbed jaw-neck behaviour in whiplash-associated disorders (WAD). The results show that neck function is an integral part of natural jaw behaviour, and that neck injury can impair jaw function and therefore disturb eating behaviour. We also show preliminary results from implementation of a new approach for rehabilitation of jaw-neck dysfunction and pain in WAD.
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| 10. |
- Jönsson, Daniel, et al.
(författare)
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Demonstration of mitochondrial oestrogen receptor beta and oestrogen-induced attenuation of cytochrome c oxidase subunit I expression in human periodontal ligament cells.
- 2007
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 52:7, s. 669-676
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: Periodontal ligament (PDL) cells express oestrogen receptor beta (ERbeta) protein, but cellular functions regulated by ERbeta in these cells have not been identified. In this study we determine if ERbeta is localised to mitochondria and if oestrogen regulates mitochondrial function in human PDL cells obtained from teeth extracted for orthodontic reasons. DESIGN: Subcellular distribution of ERbeta was determined by confocal microscopy of cells co-stained with ERbeta antibody and the mitochondrion-selective probe MitoTracker and by immunogold electron microscopy. Expression of the mitochondrial enzyme cytochrome c oxidase subunit I, involved in oxidative phosphorylation, was determined by Western blotting in cells treated with or without physiological concentrations of the endogenous oestrogen 17beta-oestradiol. RESULTS: ERbeta immunoreactivity was observed both in the nuclei and the cytoplasm. MitoTracker-labelling was observed in the cytoplasm, especially in the perinuclear region, but not in the nuclei. Co-localisation of ERbeta and MitoTracker was observed in cells derived from both male and female subjects. Mitochondrial localisation of ERbeta was confirmed by immunogold electron microscopy. Cells treated with or without 17beta-oestradiol (100 nM) displayed an identical pattern of staining for mitochondria. Treatment with 100 nM 17beta-oestradiol attenuated cytochrome c oxidase subunit I expression by about 30%, while combined treatment with 17beta-oestradiol and the ER blocker ICI 182780 (10 microM) had no effect. CONCLUSION: This study demonstrates mitochondrial localisation of ERbeta and oestrogen-induced decrease in the expression of cytochrome c oxidase subunit I in human PDL cells, suggesting that oestrogen probably via ERbeta influences mitochondrial function and PDL cell energy
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