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| 2. |
- Bechtloff, D, et al.
(författare)
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Analysis of protein synthesis rates after initiation of chromosome replication in Escherichia coli
- 1999
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Ingår i: JOURNAL OF BACTERIOLOGY. - : AMER SOC MICROBIOLOGY. - 0021-9193. ; 181:20, s. 6292-6299
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The aim of this study was to investigate whether the synthesis rates of some proteins change after the initiation of replication in Escherichia coli. An intR1 strain, in which chromosome replication is under the control of an R1 replicon integrated into a
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| 3. |
- Belitsky, B R, et al.
(författare)
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An lrp-like gene of Bacillus subtilis involved in branched-chain amino acid transport
- 1997
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Ingår i: Journal of Bacteriology. - 0021-9193. ; 179:17, s. 5448-5457
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Tidskriftsartikel (refereegranskat)abstract
- The azlB locus of Bacillus subtilis was defined previously by a mutation conferring resistance to a leucine analog, 4-azaleucine (J. B. Ward, Jr., and S. A. Zahler, J. Bacteriol. 116:727-735, 1973). In this report, azlB is shown to be the first gene of an operon apparently involved in branched-chain amino acid transport. The product of the azlB gene is an Lrp-like protein that negatively regulates expression of the azlBCDEF operon. Resistance to 4-azaleucine in azlB mutants is due to overproduction of AzlC and AzlD, two novel hydrophobic proteins.
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| 4. |
- Bengtsson, Jenny, et al.
(författare)
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Subunit II of Bacillus subtilis cytochrome c oxidase is a lipoprotein
- 1999
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Ingår i: Journal of Bacteriology. - 0021-9193. ; 181, s. 685-688
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Tidskriftsartikel (refereegranskat)abstract
- The sequence of the N-terminal end of the deduced ctaC gene product of Bacillus species has the features of a bacterial lipoprotein. CtaC is the subunit II of cytochrome caa3, which is a cytochrome c oxidase. Using Bacillus subtilis mutants blocked in lipoprotein synthesis, we show that CtaC is a lipoprotein and that synthesis of the membrane-bound protein and covalent binding of heme to the cytochrome c domain is not dependent on processing at the N-terminal part of the protein. Mutants blocked in prolipoprotein diacylglyceryl transferase (Lgt) or signal peptidase type II (Lsp) are, however, deficient in cytochrome caa3 enzyme activity. Removal of the signal peptide from the CtaC polypeptide, but not lipid modification, is seemingly required for formation of functional enzyme.
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| 5. |
- Bernander, R, et al.
(författare)
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Cell cycle characteristics of thermophilic Archaea
- 1997
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Ingår i: JOURNAL OF BACTERIOLOGY. - : AMER SOC MICROBIOLOGY. - 0021-9193. ; 179:16, s. 4963-4969
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- We have performed a cell cycle analysis of organisms from the Archaea domain, Exponentially growing cells of the thermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius were analyzed by flow cytometry, and several unusual cell cycle cha
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| 6. |
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| 7. |
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| 8. |
- Botello, E, et al.
(författare)
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Effects of chromosome underreplication on cell division in Escherichia coli
- 1998
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Ingår i: JOURNAL OF BACTERIOLOGY. - : AMER SOC MICROBIOLOGY. - 0021-9193. ; 180:23, s. 6364-6374
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The key processes of the bacterial cell cycle are controlled and coordinated to match cellular mass growth. We have studied the coordination between replication and cell division by using a temperature-controlled Escherichia coli intR1 strain. In this str
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| 9. |
- Carlson, K, et al.
(författare)
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Short-range and long-range context effects on coliphage T4 endonuclease II-dependent restriction
- 1996
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Ingår i: JOURNAL OF BACTERIOLOGY. - : AMER SOC MICROBIOLOGY. - 0021-9193. ; 178:22, s. 6419-6426
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Synthetic sites inserted into a plasmid were used to analyze the sequence requirements for in vivo DNA cleavage dependent on bacteriophage T4 endonuclease II. A 16-bp variable sequence surrounding the cleavage site was sufficient for cleavage, although co
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| 10. |
- Charpentier, Emmanuelle, et al.
(författare)
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Conjugative mobilization of the rolling-circle plasmid pIP823 from Listeria monocytogenes BM4293 among gram-positive and gram-negative bacteria.
- 1999
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Ingår i: Journal of Bacteriology. - 0021-9193 .- 1098-5530. ; 181:11, s. 3368-3374
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Tidskriftsartikel (refereegranskat)abstract
- We determined the sequence and genetic organization of plasmid pIP823, which contains the dfrD gene; dfrD confers high-level trimethoprim resistance to Listeria monocytogenes BM4293 by synthesis of dihydrofolate reductase type S2. pIP823 possessed all the features of the pUB110/pC194 plasmid family, whose members replicate by the rolling-circle mechanism. The rep gene encoded a protein identical to RepU, the protein required for initiation of the replication of plasmids pTB913 from a thermophilic Bacillus sp. and pUB110 from Staphylococcus aureus. The mob gene encoded a protein with a high degree of amino acid identity with the Mob proteins involved in conjugative mobilization and interplasmidic recombination of pTB913 and pUB110. The host range of pIP823 was broad and included L. monocytogenes, Enterococcus faecalis, S. aureus, Bacillus subtilis, and Escherichia coli. In all these species, pIP823 replicated by generating single-stranded DNA and was stable. Conjugative mobilization of pIP823 was obtained by self-transferable plasmids between L. monocytogenes and E. faecalis, between L. monocytogenes and E. coli, and between strains of E. coli, and by the streptococcal conjugative transposon Tn1545 from L. monocytogenes to E. faecalis, and from L. monocytogenes and E. faecalis to E. coli. These data indicate that the gene flux observed in nature from gram-positive to gram-negative bacteria can occur by conjugative mobilization. Our results suggest that dissemination of trimethoprim resistance in Listeria spp. and acquisition of other antibiotic resistance determinants in this species can be anticipated.
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