| 1. |
- Brodelius, Peter, et al.
(författare)
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A Procedure for the Determination of Optimal Chitosan Concentrations for Elicitation of Cultured Plant Cells.
- 1989
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 28:10, s. 2651-2654
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Tidskriftsartikel (refereegranskat)abstract
- An experimental method for determination of the optimum chitosan concentration for elicitation of plant cell suspension cultures is presented. The procedure, which is based on measurements of the conductivity of the culture medium after addition of chitosan, has been applied to suspension cultures of Nicotiana tabacum and Eschscholtzia californica. Increased conductivity of the medium (due to permeabilization of the cells) results in decreased secondary product formation and cell growth. Maximum product formation is observed for cells elicited with the highest chitosan concentration which does not affect membrane permeability.
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| 2. |
- Collinge, M A, et al.
(författare)
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Dynamics of Benzophenanthridine Alkaloid Production in Suspension Cultures of Eschscholtzia californica after Treatment with a Yeast Elicitor
- 1989
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 28:4, s. 1101-1104
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Tidskriftsartikel (refereegranskat)abstract
- A number of benzophenanthridine alkaloids are induced in suspension cultures of Eschscholtzia californica after treatment with an elicitor prepared from yeast extract. The formation of the alkaloids sanguinarine, chelerythrine and macarpine has been studied in relation to; elicitor concentration, incubation time after elicitation, and culture age. A significant portion of these alkaloids is released into the medium. Sanguinarine and chelerythrine reach maximum levels a few hours after the time of elicitation. Thereafter, their levels decline and the amount of macarpine increases. Viability of elicited cells, as determined by their subsequent growth, is not significantly reduced. There is a good correlation between induced tyrosine decarboxylase activity and alkaloid formation.
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| 3. |
- Funk, C, et al.
(författare)
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Increased Secondary Product Formation in Plant Cell Suspension Cultures after Treatment with a Yeast Carbohydrate (Elicitor)
- 1987
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 26, s. 401-405
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Tidskriftsartikel (refereegranskat)abstract
- A carbohydrate fraction isolated from yeast extract by ethanolic precipitation was used as an elicitor to induce secondary product formation in plant cell suspension cultures. The elicitor preparation is effective in inducing glyceollin isomer synthesis (up to 200 μg glyceollin per g dry wt) in cells of Glycine max and enhancing berberine biosynthesis (up to four-fold) in cells of Thalictrum rugosum. The response of the cell cultures to the elicitor treatment is dependent on the amount of carbohydrate per unit of biomass and on the physiological state of the cells. Cells are optimally induced in late exponential or early stationary growth phases.
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| 4. |
- Haldimann, D, et al.
(författare)
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Redirecting Cellular Metabolism by Immobilization of Cultured Plant Cells: A Model Study with Coffea arabica
- 1987
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 26:5, s. 1431-1434
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Tidskriftsartikel (refereegranskat)abstract
- Suspension-cultured cells of Coffea arabica have been immobilized by entrapment in calcium alginate gels to mimic natural aggregation. The production of methylxanthine alkaloid was increased up to 13-fold by the immobilization. This increased production has been ascribed to organization of the entrapped cells through physicochemical interactions between the polymer (alginate) and the plant cell wall. It has been shown that the metabolic changes induced by the immobilization are reversible.
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