| 1. |
- Downs, Mark E.A., et al.
(författare)
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Optical and electrochemical detection of DNA
- 1988
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Ingår i: Biomaterials. - : Elsevier. - 0142-9612 .- 1878-5905. ; 9:1, s. 66-70
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Tidskriftsartikel (refereegranskat)abstract
- There is a growing demand for the production of a DNA biosensor with applications in medicine, the food industry, agriculture, veterinary science and environmental science. In this paper we describe methods for the optical and electrochemical detection of DNA using the enzyme horseradish peroxidase (EC 1.11.1.7) and glucose oxidase (EC 1.1.3.4). We have used bis-methylacridinium nitrate and luminol for the optical detection of DNA using a purpose built, inexpensive luminometer. Using this system detection limits of 10−11g of plasmid DNA have been observed. Electrochemical detection of DNA was carried out by the use of a fluoride ion selective electrode and stripping voltametry. DNA was detected down to 1 (10−9 − 10−10g of DNA by the enzymatic release of halogen ions from organohalogen compounds.
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| 2. |
- Sennerby, Lars, 1960, et al.
(författare)
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Acute tissue reactions to potassium alginate with and without colour/flavour additives.
- 1987
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Ingår i: Biomaterials. - : Elsevier BV. - 0142-9612. ; 8:1, s. 49-52
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Tidskriftsartikel (refereegranskat)abstract
- The acute tissue reactions to potassium alginate, locally applied to a microvascular bed, were studied with the use of the vital microscopic hamster cheek pouch model combined with correlative histology. This experimental model permitted us to study microvascular permeability, blood flow, vessel diameters and leucocyte adhesion to vessel walls intravitally, and leucocyte migration and mastcell degranulation histologically. Deionized water alone and potassium alginate with flavour and colour mixed in saline was found to cause severe microvascular alterations, while potassium alginate, without flavour and colour, but mixed in saline and applied to the microvasculature resulted in a minor inflammatory reaction.
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