| 1. |
- Persson, B. R.R., et al.
(författare)
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Labeling and testing of (99m)Tc streptokinase for the diagnosis of deep vein thrombosis
- 1975
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Ingår i: Journal of Nuclear Medicine. - 0161-5505. ; 16:6, s. 474-477
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Tidskriftsartikel (refereegranskat)abstract
- A detailed study has been made of the method for labeling streptokinase with 99Tc(m) in order to get a radioactive indicator for rapid scintigraphic visualization of thrombi and emboli. The best method found for preparing 99Tc(m) streptokinase was by reducing 99Tc(m) pertechnetate with 2 μmole SnCl2 at pH 0.7 and then adding 50,000 to 75,000 IU of streptokinase at a final pH of about 2. After 1 hr of equilibration, the labeling efficiency was 75 to 80% as determined by the method of gel chromatography column scanning. The dynamic behavior and distribution of 99Tc(m) streptokinase in different organs was studied in rabbits where a high uptake was found in liver. In a clinical investigation using 99Tc(m) streptokinase prepared according to this work, however, the liver uptake in man was only 10 to 20%.
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| 3. |
- Strand, S. E., et al.
(författare)
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Quantitative lymphoscintigraphy. I. Basic concepts for optimal uptake of radiocolloids in the parasternal lymph nodes of rabbits
- 1979
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Ingår i: Journal of Nuclear Medicine. - 0161-5505. ; 20:10, s. 1038-1046
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Tidskriftsartikel (refereegranskat)abstract
- The activity-size distribution of radiocolloids has been studied using gel-chromatography scanning (GCS) of columns filled with Sepharose 4B gel. Rabbits were injected subcutaneously with the colloid of interest, laid supine beneath a gamma camera, and imaged every 15 sec for 2 to 4 hr. From the stored data, the uptakes in the parasternal lymph nodes were analyzed in terms of a two-compartment model, and the rate constants measured. The substances tested were Au-198 colloid, Tc-99m antimony sulfide colloid, Tc-99m tin colloid, Tc-99m phytate, and Tc99m sulfur colloid. It was shown that the optimal particle size for the colloid is in the range 1-10 nm. The largest and most rapid uptake was found for Au-198 colloid, with a particle size of 5 nm, which appeared as a single peak in the GCS spectrum. The percentage uptake after 2 hr for Au-198 colloid was 8%, while it was 5% for antimony sulfide colloid, which was the best of the Tc-99m-labeled colloids. The GCS spectrum for the antimony product showed a single-peaked size distribution with a somewhat broader range: 5-15 nm. The particles of the other colloids were either too large to pass into the lymphatic system, or too small to be trapped.
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