Gibberellic acid-induced changes in glutathione metabolism and anthocyanin content in plant tissue

• Literature data point to a possible link between gibberellic acid (GA(3)) and glutathione metabolism in plant tissue, as both are connected to dormancy breakage. In order to study the influence of GA(3) on glutathione metabolism, we treated an anthocyanin accumulating cell culture of periwinkle (Catharanthus roseus) and a shoot differentiated culture of pea (Pisum sativum) with GA(3). Glutathione reductase (GR; E.C. 1.6.4.2) activity increased to 135% and 190% of the control in C. roseus and P. sativum, respectively. The level of oxidized glutathione (GSSG) decreased to 60% of the control in the C. resells culture while no change in GSSG was observed in the P. sativum culture. No changes in the tissue concentration of total glutathione was observed in the cultures after GA(3) treatment. Concomitant. to the changes in GSSG and GR, an increase in anthocyanin accumulation was observed in the C. roseus culture in association with a strong increase in phenylalanine ammonia-lyase (PAL; E.C. 4.3.1.5) activity in response to GA(3). These data strongly suggest a link between GA(3) and glutathione metabolism.

Nyckelord

anthocyanins

gibberellins

glutathione reductase

oxidized glutathione

phenylalanine ammonia-lyase

reductase

cultures

stress

pea

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