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- Zhao, Song, et al.
(författare)
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Organ bath in detecting the effect of one-hour warm ischemia on pulmonic arteries and bronchi from non-heart-beating donor lungs
- 2009
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Ingår i: Chinese Medical Journal. - 0366-6999. ; 122:23, s. 2903-2906
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Tidskriftsartikel (refereegranskat)abstract
- Background Non-heart-beating donor lung has been a promising source of lung transplantation. Many studies on non-heart-beating donor lungs are based on animal lung transplantation. In this study, we assessed by organ bath the effect of one-hour warm ischemia on the non-heart-beating donor lung in terms of the integrity of contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi. Methods Sixteen Swedish pigs were randomly classified into two groups: heart-beating donor group and 1-hour warm ischemia non-heart-beating donor group. Pulmonic and bronchial rings were taken from the isolated left lungs of the pigs. The pulmonic rings were stimulated by U-46619 (5.7 mol/L) and acetylcholine (10(-4) mmol/L) to assess the contractile abilities of smooth muscle and the endothelium-dependent relaxation response, respectively. As such, acetylcholine (10(-5) mmol/L) and natrium arachidonic acid (0.01%) were used to detect the contraction of bronchial smooth muscle and epithelium-dependent relaxation response. Meanwhile, the variances of precontraction tension of control groups were recorded to measure whether there was spontaneous relaxation during endothelium/epithelium-dependent relaxation course. Finally, papaverine solution (10(-4) mmol/L) was used to detect the non-endothelium/epithelium-dependent relaxant abilities of pulmonic and bronchial smooth muscles. Results There was no significant difference in the tension values of precontraction of pulmonic rings (P >0.05), endothelium-dependent relaxation (P >0.05), precontraction of bronchial rings (P >0.05) and epithelium-dependent relaxation (P >0.05) between the heart-beating donor group and the 1-hour warm ischemia non-heart-beating donor group. And the pulmonic and bronchial rings of each subgroup B had no spontaneous relaxation. Finally, papaverine solution relaxed the smooth muscle of all the rings completely. Conclusions The results of this experiment suggest that the contractile and relaxant functions and tissue structures of pulmonic arteries and bronchi are not damaged after warm ischemia for 1 hour, and support the further study of ;non-heart-beating donor lung.
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| 2. |
- Liu, Jin-ming, et al.
(författare)
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Mid-term effects of lung volume reduction surgery on pulmonary function in patients with chronic obstructive pulmonary disease
- 2007
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Ingår i: Chinese Medical Journal. - 0366-6999. ; 120:8, s. 658-662
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Tidskriftsartikel (refereegranskat)abstract
- Background Now lung volume reduction surgery (LVRS) has become one of the most effective methods for the management of some cases of severe chronic obstructive pulmonary disease (COPD). We evaluated the mid-term effects of LVRS on pulmonary function in patients with severe COPD. Methods Ten male patients with severe COPD aged 38-70 years underwent LVRS and their pulmonary function was assessed before, 3 months and 3 years after surgery. The spirometric and gas exchange parameters included residual volume, total lung capacity, inspiratory capacity, forced vital capacity, forced expiratory volume in one second, diffusion capacity for CO, and arterial blood gas. A 6-minute walk distance (6MWD) test was performed. Results As to preoperative assessment, most spirometric parameters and 6MWD were significantly improved after 3 months and slightly 3 years after LVRS. Gas exchange parameters were significantly improved 3 months after surgery, but returned to the preoperative levels after 3 years. Conclusions LVRS may significantly improve pulmonary function in patients with severe COPD indicating for LVRS. Mid-term pulmonary function 3 years after surgery can be decreased to the level at 3 months after surgery. Three years after LVRS, lung volume and pulmonary ventilation function can be significantly improved, but the improvement in gas exchange function was not significant.
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| 3. |
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| 4. |
- Shi, WF, et al.
(författare)
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Inhibitory effects of reserpine and carbonyl cyanide m-chloro-phenylhydrazone on fluoroquinolone resistance of Acinetobacter baumannii
- 2005
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Ingår i: Chinese Medical Journal. - 0366-6999. ; 118:4, s. 340-343
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Tidskriftsartikel (refereegranskat)abstract
- Mechanisms of bacterial resistance to fluoroquinolones may be grouped into three principal categories: gene mutations of DNA topoisomerase II (GyrA or GyrB), DNA topoisomerase IV (ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug efflux pump (active efflux system).(1) Efflux pumps are transport proteins removing toxic substrates ( including virtually all classes of clinically relevant antibiotics) from cells to the external environment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as well as in fungi and mammalian (tumour) cells.(2-4) It has been reported that alkaloid reserpine and carbonyl cyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux.(5,6) In order to explore the universality of drug efflux in microorganisms, 85 strains of Acinetobacter baumannii (A. baumannii) were tested using reserpine and CCCP. The quinolone-resistant-determining region (QRDR) of gyrA and parC genes in 35 isolates of A. baumannii were amplified by polymerase chain reaction (PCR) and sequenced by DNA sequencer. The correlation between resistant mutation regularity and bacterial drug efflux were analysed.
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