| 1. |
- Börjesson, J., et al.
(author)
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X-ray fluorescence analysis in medical sciences: A review
- 2003
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In: Acta Diabetologica. - : Springer Science and Business Media LLC. - 0940-5429 .- 1432-5233. ; 40
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Journal article (peer-reviewed)abstract
- Some elements have toxic effects on the human body and there is thus a need to control their levels in human organs and tissues. Moreover, it is important to increase our knowledge of relationships between observable toxic effects and element concentrations in man and his environment. Monitoring and basic occupational and environmental research rely on measurements directly in humans as well as samples from humans and the environment. This paper reviews recent advances in in vivo X-ray fluorescence methods and their applications.
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| 2. |
- Fugmann, Andreas, et al.
(author)
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Blood flow is an important determinant of forearm glucose uptake following a mixed meal
- 2003
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In: Acta Diabetologica. - : Springer Science and Business Media LLC. - 0940-5429 .- 1432-5233. ; 40:3, s. 113-117
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Journal article (peer-reviewed)abstract
- Insulin-mediated vasodilation has been suggested to be of importance for glucose uptake during normoglycemic hyperinsulinemia. If this also is valid after an ordinary mixed meal remains to be evaluated. Forearm blood flow (FBF) and forearm glucose uptake change (evaluated by venous occlusion plethysmography) and glucose arteriovenous differences were evaluated over 120 minutes in 10 healthy volunteers following an ordinary mixed meal (700-900 kcal, 34% of energy from fat). Fasting arterial glucose level was 4.9+/-0.9 mmol/l, and the maximum glucose level was reached 30 minutes after the start of ingestion (6.6+/-0.8 mmol/l, p<0.0001). Plasma insulin levels were increased four-fold. FBF increased rapidly within 20 minutes after the start of ingestion and reached its maximum after 50 minutes (94% higher than baseline level, p<0.01). After 2 hours FBF was still substantially elevated (75% above baseline level, p<0.01). Forearm glucose uptake increased fivefold already after 20 minutes ( p<0.01). During the 2 hours, the increase in FBF contributed to 41% of the forearm glucose uptake ( p<0.05). The present study showed that the increase in FBF seen after an ordinary mixed meal is important for the change in forearm glucose uptake. These results support the view that modulation of limb blood flow is a determinant of glucose uptake.
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| 3. |
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| 4. |
- Isaksson, Mats, 1961, et al.
(author)
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Determination of potassium in the skeletal muscles by whole-body counting
- 2003
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In: Acta Diabetologica. - : Springer Science and Business Media LLC. - 0940-5429 .- 1432-5233. ; 40
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Journal article (peer-reviewed)abstract
- Changes in muscular function are related to nutritional status, disease and physical activity. To study these relationships, it is desirable to be able to determine the whole body potassium content, which is characteristic to the muscular tissue. This can be achieved by measurements in a whole-body counter, identifying contributions from the upper and lower parts of the body. In a whole-body counter with large plastic scintillators, a special measuring methodology is required. Such a method of measuring 40K in the leg muscles, extracting the part of the detector signal originating from the lower part of the body, has been developed and tested by independent phantom measurements. The results suggest that it is suited to perform regional measurements of body potassium but validation and implementation into clinical research are still necessary.
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| 5. |
- Larsson-Nyrén, G, et al.
(author)
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Anion-selective amplification of glucose-induced insulin secretion.
- 2002
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In: Acta Diabetologica. - 0940-5429 .- 1432-5233. ; 39:1, s. 41-7
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Journal article (peer-reviewed)abstract
- The functional roles of anions on glucose-induced insulin secretion are poorly understood. We investigated the effects of the monovalent anions thiocyanate, iodide, bromide, nitrate and chloride on the dynamics of insulin secretion in isolated pancreatic islets from non-inbred Umeå ob/ob mice. All anion species (12 mM), except Cl-, significantly amplified glucose-induced (20 mM) first- and second-phase insulin secretion (selectivity sequence: SCN->NO3->I->Br->Cl-). Simultaneously, the anions reduced the lag-time prior to the initiation of the secretion (SCN-=I-=NO3->Br->Cl-). The results indicate that pancreatic beta-cell activation can be initiated and amplified by an anion-selective mechanism showing increasing degrees of activation in the order of the anion series of Hofmeister. On the basis of the strikingly similar anion selectivity of amplified secretion and shortened lag-phase, we suggest that both types of anion effects are caused by action at a single site on the beta-cell.
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| 6. |
- Persson-Sjögren, Solveig, et al.
(author)
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Effects of leptin, acetylcholine and vasoactive intestinal polypeptide on insulin secretion in isolated ob/ob mouse pancreatic islets
- 2004
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In: Acta Diabetologica. - Berlin : Springer. - 0940-5429 .- 1432-5233. ; 41:3, s. 104-112
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Journal article (peer-reviewed)abstract
- Obesity is often accompanied by hyperleptinemia, hyperinsulinemia, and an increased parasympathetic tone. Obese-hyperglycemic mice (Umeå ob/ob) have functional leptin receptors and a raised parasympathetic tone. We studied insulin release in islets isolated from 9-month-old severely obese ob/ob mice. Leptin (0.5-18 nM) did not affect insulin release together with 2.8-20 mM glucose. Leptin (18 microM) had no effect in the presence of low glucose (2.8-5.5 mM), but increased insulin secretion in islets challenged with 11.1 or 16.7 mM glucose. Leptin at 18 microM increased insulin secretion stimulated by the parasympathetic neurotransmitters acetylcholine (ACh; 10 microM) or vasoactive intestinal peptide (VIP; 10 nM), and by 5 mM theophylline or 2.5 microM forskolin. Overnight culture increased the effect of 18 microM leptin, but no effects were observed with 18 nM leptin. Pretreatment of islets with phorbol 12-myristate 13-acetate (PMA) did not suggest any involvement of protein kinase C. In summary, a high concentration of leptin stimulates insulin release in the presence of stimulatory concentrations of glucose alone and with parasympathetic neurotransmitters. Hyperleptinemia and increased parasympathetic stimulation may in part cause the hyperinsulinemia observed in obesity. This may aggravate insulin resistance and the abnormal metabolism in diabetes mellitus.
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| 7. |
- Rothenberg, Elisabet, et al.
(author)
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Energy expenditure at age 73 and 78 : a five year follow-up
- 2003
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In: Acta Diabetologica. - 0940-5429 .- 1432-5233. ; 40, s. S134-S138
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Journal article (peer-reviewed)abstract
- There is limited knowledge concerning the energy turnover and energy requirements of the elderly. The aim of this study was to measure resting metabolic rate (RMR) and total energy expenditure (TEE), and to define activity energy expenditure (AEE) and physical activity level (PAL) in 11 78-year-old individuals. A further aim was to compare these measures with the same measures taken from the 11 subjects at age 73 years. Total body water (TBW) averaged 30.8 kg (SD 3.2) at age 73 and 28.8 kg (SD 3.5) at age 78 years (p<0.01). RMR was measured with a ventilated-hood system and averaged 5.60 MJ/day (SD 0.66) at age 73 and 4.77 MJ/day (SD 0.32) at 78 years (p<0.001). TEE, measured using the doubly labeled water method, averaged 9.64 MJ/day (SD 1.10) at age 73 and 8.30 MJ/day (SD 1.45) at 78 years (p<0.05). AEE averaged 4.04 MJ/day (SD 0.44) at age 73 and 3.53 MJ/day (1.13) at 78 years (ns). PAL averaged 1.74 at both ages. TBW, TEE, and RMR decreased, whereas AEE and PAL did not change substantially during the 5-year follow-up. The PAL values indicated a physically active lifestyle for the age group.
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| 8. |
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| 9. |
- Johansson, Torsten, et al.
(author)
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Heterotopic bone formation following internal fixation or arthroplasty for displaced femoral neck fractures: a prospective randomized study
- 2001
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In: International Orthopaedics. - : Springer Science and Business Media LLC. - 0341-2695 .- 1432-5195. ; 25:4, s. 223-225
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Journal article (peer-reviewed)abstract
- One hundred hips in 99 patients of 75 years or older, with a displaced femoral neck fracture, were studied for heterotopic ossification (HO). The patients were randomized to either internal fixation or total hip arthroplasty (THA). In the THA group HO was found in 32 of 45 hips compared with 1 of 39 in the internal fixation group (P<0.0012). The frequency of HO after THA corresponds well with findings in other studies on patients receiving THA for osteoarthrosis. In cervical fractures the surgical procedure of total hip replacement seems to be a prerequisite for HO, indicating that the procedure itself is more important than the patient's age and the diagnosis. Severe symptoms due to HO were found in only one patient. HO following THA for a femoral neck fracture is of little clinical importance and prophylaxis is unnecessary.
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