| 1. |
- Turner, Anthony P. F., et al.
(författare)
-
Biosensors and Bioelectronics' impact grows
- 2005
-
Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 21:6, s. 839-839
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
| 2. |
- Ahmad, Farook, et al.
(författare)
-
Minimizing tissue-material interaction in microsensor for subcutaneous glucose monitoring
- 2007
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 22:8, s. 1625-1632
-
Tidskriftsartikel (refereegranskat)abstract
- A new implantable electrocatalytic glucose sensor for subcutaneous glucose monitoring has been fabricated by immobilizing glucose oxidase on a chemically modified carbon fiber. The sensor was inserted subcutaneously on a male spraguely rat without any incision after dipping the microsensor in the rat's serum for 3 days. The so called "stained" microsensor, operated in the amperometric mode with an applied potential of +0.23 V versus Ag vertical bar AgCl, was able to directly measure the glucose concentration upon infusion of glucose. The results obtained were encouraging, with the response time was less than 2 s and the apparent Michaelis-Menten value at 5.1 +/- 0.5 mM. The "stained" microsensor shows good stability and reproducibility with constant response spanned over 25 days. Most common interferences in glucose analysis were minimized by the outerlayer Nafion((R)). Hematology examinations showed minimal material-tissue interaction. Use of such mechanical devices will allow a more refined understanding towards glucose control in diabetic patients as the implanted microsensor was not effected by biocompatibility failures. (c) 2006 Elsevier B.V. All rights reserved.
|
|
| 3. |
- Allender, Chris, et al.
(författare)
-
MIP2008 Kobe, Japan, September 2008 preface
- 2009
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 25:3, s. 539-542
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
| 4. |
- Alpeeva, IS, et al.
(författare)
-
Palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring
- 2005
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 21:5, s. 742-748
-
Tidskriftsartikel (refereegranskat)abstract
- Three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (RPTP), anionic sweet potato peroxidase (SPP), or cationic horseradish peroxidase (HRP-C) on spectroscopic graphite electrodes. The resulting H2O2-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as pH dependency,I-max, K-M(app), detection limit, linear range, operational and storage stability. The obtained results showed a distinctly different behavior for the plant peroxidase electrodes, demonstrating uniquely superior characteristics of the RPTP-based sensors. The broader linear range observed for the RPTP-based biosensor is explained by a high stability of this enzyme in presence of H2O2. The higher storage and operational stability of RPTP-based biosensor as well as its capability to measure hydrogen peroxide under acidic conditions connect with an extremely high thermal and pH-stability of RPTP.
|
|
| 5. |
|
|
| 6. |
- Anderson, Tony, 1973-, et al.
(författare)
-
Frog melanophores cultured on fluorescent microbeads : Biomimic-based biosensing
- 2005
-
Ingår i: Biosensors & bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 21:1, s. 111-120
-
Tidskriftsartikel (refereegranskat)abstract
- Melanophores are pigmented cells in lower vertebrates capable of quick color changes and thereby suitable as whole cell biosensors. In the frog dermis skin layer, the large and dark pigmented melanophore surrounds a core of other pigmented cells. Upon hormonal stimulation the black-brown pigment organelles will redistribute within the melanophore, and thereby cover or uncover the core, making complex color changes possible in the dermis. Previously, melanophores have only been cultured on flat surfaces. Here we mimic the three dimensional biological geometry in the frog dermis by culturing melanophores on fluorescent plastic microbeads. To demonstrate biosensing we use the hormones melatonin and α-melanocyte stimulating hormone (α-MSH) as lightening or darkening stimuli, respectively. Cellular responses were successfully demonstrated on single cell level by fluorescence microscopy, and in cell suspension by a fluorescence microplate reader and a previously demonstrated computer screen photo-assisted technique. The demonstrated principle is the first step towards "single well/multiple read-out" biosensor arrays based on suspensions of different selective-responding melanophores, each cultured on microbeads with distinctive spectral characteristics. By applying small amount of a clinical sample, or a candidate substance in early drug screening, to a single well containing combinations of melanophores on beads, multiple parameter read-outs will be possible. © 2004 Elsevier B.V. All rights reserved.
|
|
| 7. |
- Antiochia, Riccarda, et al.
(författare)
-
Development of a carbon nanotube paste electrode osmium polymer-mediated biosensor for determination of glucose in alcoholic beverages
- 2007
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 22:11, s. 2611-2617
-
Tidskriftsartikel (refereegranskat)abstract
- This paper describes a new amperometric biosensor for glucose monitoring. The biosensor is based on the activity of glucose dehydrogenase (GDH) and diaphorase (DI) co-immobilized with NAD(+) into a carbon nanotube paste (CNTP) electrode modified with an osmium functionalized polymer. This mediator was demonstrated to shuttle the electron transfer between the immobilized diaphorase and the CNTP electrode, thus, showing a good electrocatalytic activity towards NADH oxidation at potentials around +0.2 V versus Ag vertical bar AgCl, where interfering reactions are less prone to occur. The biosensor exhibits a detection limit of 10 mu mol L-1, linearity up to 8 x 10(-4) mol L-1, a sensitivity of 13.4 mu A cm(-2) mmol(-1) L-1, a good reproducibility (R.S.D. 2.1%, n = 6) and a stability of about 1 week when stored dry at 4 degrees C. Finally, the proposed biosensor was applied for the determination of glucose in different samples of sweet wine and validated with a commercial spectrophotometric enzymatic kit. (c) 2006 Elsevier B.V. All rights reserved.
|
|
| 8. |
- Asif, Muhammad, 1978-, et al.
(författare)
-
Selective calcium ion detection with functionalized ZnO nanorods-extendedgate MOSFET
- 2009
-
Ingår i: Biosensors & bioelectronics. - : ELSEVIER. - 0956-5663 .- 1873-4235. ; 24:11, s. 3379-3382
-
Tidskriftsartikel (refereegranskat)abstract
- Zinc oxide nanorod-extended gate field effect transistor (MOSFET) is demonstrated for the detection of calcium (Ca2+) ions. ZnO nanorods were grown on the surface of a silver wire to produce an electrochemical nanosensor for selectively detecting Ca2+. The electrochemical response from the interaction between the ZnO nanorods and Ca2+ in an aqueous solution is coupled directly to the gate of a field effect transistor (MOSFET). The induced voltage change on the gate results in a measureable current response. In order to adapt the sensors for Ca2+ ions measurements in biological fluids with sufficient selectivity and stability, a plastic membrane coating containing ionophores was applied on the nanorods. The sensor exhibited a linear response within the range of interest from 1 μM to 1 mM. This work demonstrates a simple technique for sensitive detection of Ca2+ ions by efficient transfer of the chemical response directly to a standard electronic component producing a low impedance signal.
|
|
| 9. |
- Belmont, Anne-Sophie, et al.
(författare)
-
Molecularly imprinted polymer films for reflectometric interference spectroscopic sensors
- 2007
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 22:12, s. 3267-3272
-
Tidskriftsartikel (refereegranskat)abstract
- Reflectometric interference spectroscopic measurements were performed on molecularly imprinted polymer (MIP) films with the herbicide atrazine as the template molecule. A conventional imprinting protocol was used relying on non-covalent interactions between the functional monomers and the template. The MIPs were deposited on glass transducers by two different methods: spin-coating followed by in situ polymerization of thin films of monomers containing a sacrificial polymeric porogen, and autoassembly of MIP nanoparticles with the aid of an associative linear polymer. Reproducible results were obtained upon measurements of atrazine solutions in toluene with both films. Atrazine concentrations as low as 1.7 pprn could be detected with the autoassembled particle film. No or very little analyte adsorption was observed onto non-imprinted control films made by spin-coating and by particle assembly, respectively. We believe that these MIP layers in combination with the general reflectrometric transduction scheme could be a versatile sensing tool for the detection of environmentally important and other analytes. (c) 2007 Elsevier B.V. All rights reserved.
|
|
| 10. |
- Beni, Valerio, et al.
(författare)
-
Cyclic and pulse voltammetric study of dopamine at the interfacebetween two immiscible electrolyte solutions
- 2005
-
Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 20:10, s. 2097-2103
-
Tidskriftsartikel (refereegranskat)abstract
- The detection of dopamine by differential pulse voltammetry (DPV) and square wave voltammetry (SWV) at the interface between twoimmiscible electrolyte solutions (ITIES) has been studied. Voltammetry at the liquid/liquid (water/1,2-dichloroethane) interface provides asimple method for overcoming the major problem associated with dopamine detection by voltammetry at solid electrodes: the co-existenceof ascorbate at higher concentrations. Selectivity for dopamine was achieved by the use of dibenzo-18-crown-6 as an ionophore for thefacilitated transfer voltammetry of protonated dopamine across the ITIES. Under these conditions, ascorbate is not transferred and hence doesnot interfere in the ion transfer current for dopamine. By use of DPV and SWV, the lowest concentration detectable can be lowered from ca.0.1mM (obtained with cyclic voltammetry) to 2 M. Evaluation of the effect of some other physiologically important species (acetylcholine,sodium, potassium and ammonium ions) on the dopamine transfer voltammetry has been studied, indicating the need for improved ionophoredesigns in order to achieve practically useful selectivity.
|
|
