| 1. |
- Baltzer, Lars, et al.
(författare)
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Emerging principles of de novo catalyst design
- 2001
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Ingår i: Current Opinion in Biotechnology. - 0958-1669 .- 1879-0429. ; 12:4, s. 355-360
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Forskningsöversikt (refereegranskat)abstract
- Considerable progress has been made in the understanding of how to exploit hydrophobic and charge - charge interactions in forming binding sites for peptides and small molecules in folded polypeptide catalysts. This knowledge has enabled the introduction of feedback and control functions into catalytic cycles and the construction of folded polypeptide catalysts that follow saturation kinetics. Major advances have also been made in the design of metalloproteins and metallopeptides, especially with regards to understanding redox potential control.
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| 2. |
- Hult, Karl, et al.
(författare)
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Engineered enzymes for improved organic synthesis
- 2003
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 14:4, s. 395-400
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Forskningsöversikt (refereegranskat)abstract
- Recent developments to modify enzymes for use in organic synthesis have targeted several areas. These include altering the reaction mechanism of the enzyme to catalyse new reactions, switching substrate specificity, expanding substrate specificity, and improving substrate specificity, such as enantioselectivity in kinetic resolutions. Such modifications can be achieved either by rational redesign, which requires knowledge of the enzyme structure, or by random mutagenesis methods followed by screening. Both strategies of enzyme engineering can be successful and are very useful for improving the utility of enzymes for applied catalysis. Several examples illustrating these concepts in a variety of enzyme classes have appeared recently.
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| 3. |
- Andersson, Helene, et al.
(författare)
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Microtechnologies and nanotechnologies for single-cell analysis
- 2004
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 15:1, s. 44-49
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Forskningsöversikt (refereegranskat)abstract
- Many efforts are currently underway to try and mimic the properties of single cells with the aim of designing chips that are as efficient as cells. However, cells are nature's nanotechnology engineering at the scale of atoms and molecules, and it might be better to envision a microchip that utilizes a single cell as an experimentation platform. A novel, so-called laboratory-in-a-cell concept has been described, where advantage is taken of micro-and nanotechnological tools to enable precise control of the biochemical cellular environment; these tools also offer the possibility to analyse the composition of single cells. Methods for single-cell handling and analysis are being developed and will be required for this concept to progress further.
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