| 1. |
- Blomberg, Anders, 1956
(författare)
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Measuring growth rate in high-throughput growth phenotyping.
- 2010
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Ingår i: Current opinion in biotechnology. - : Elsevier BV. - 1879-0429 .- 0958-1669. ; 22:1, s. 94-102
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Forskningsöversikt (refereegranskat)abstract
- Growth rate is an important variable and parameter in biology with a central role in evolutionary, functional genomics, and systems biology studies. In this review the pros and cons of the different technologies presently available for high-throughput measurements of growth rate are discussed. Growth rate can be measured in liquid microcultivation of individual strains, in competition between strains, as growing colonies on agar, as division of individual cells, and estimated from molecular reporters. Irrespective of methodology, statistical issues such as spatial biases and batch effects are crucial to investigate and correct for to ensure low false discovery rates. The rather low correlations between studies indicate that cross-laboratory comparison and standardization are pressing issue to assure high-quality and comparable growth-rate data.
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| 2. |
- Chen, Yun, 1978, et al.
(författare)
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Advances in metabolic pathway and strain engineering paving the way for sustainable production of chemical building blocks
- 2013
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 24:6, s. 965-972
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Forskningsöversikt (refereegranskat)abstract
- Bio-based production of chemical building blocks from renewable resources is an attractive alternative to petroleum-based platform chemicals. Metabolic pathway and strain engineering is the key element in constructing robust microbial chemical factories within the constraints of cost effective production. Here we discuss how the development of computational algorithms, novel modules and methods, omics-based techniques combined with modeling refinement are enabling reduction in development time and thus advance the field of industrial biotechnology. We further discuss how recent technological developments contribute to the development of novel cell factories for the production of the building block chemicals: adipic acid, succinic acid and 3-hydroxypropionic acid.
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| 3. |
- Cordell, Dana, et al.
(författare)
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The phosphorus mass balance : identifying ‘hotspots’ in the food system as a roadmap to phosphorus security
- 2012
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Ingår i: Current Opinion in Biotechnology. - Oxford, United Kingdom : Elsevier. - 0958-1669 .- 1879-0429. ; 23:6, s. 839-845
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Forskningsöversikt (refereegranskat)abstract
- Phosphorus is a critical element on which all life depends. Global crop production depends on fertilisers derived from phosphate rock to maintain high crop yields. Population increase, changing dietary preferences towards more meat and dairy products, and the continuing intensification of global agriculture supporting this expansion will place increasing pressure on an uncertain, but finite supply of high-quality phosphate rock. Growing concern about phosphorus scarcity and security, coupled with the environmental impact of phosphorus pollution, has encouraged an increase in research exploring how phosphorus is used and lost in the food system-from mine to field to fork. An assessment of recent phosphorus flows analyses at different geographical scales identifies the key phosphorus 'hotspots', for example within the mining, agriculture or food processing sectors, where efficiency and reuse can be substantially improved through biotechnological approaches coupled with policy changes.
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| 4. |
- de Jong, Bouke Wim, 1983, et al.
(författare)
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Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels
- 2012
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 23:4, s. 624-630
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Forskningsöversikt (refereegranskat)abstract
- Transportation fuels will gradually shift from oil based fuels towards alternative fuel resources like biofuels. Current bioethanol and biodiesel can, however, not cover the increasing demand for biofuels and there is therefore a need for advanced biofuels with superior fuel properties. Novel cell factories will provide a production platform for advanced biofuels. However, deep cellular understanding is required for improvement of current biofuel cell factories. Fast screening and analysis (-omics) methods and metabolome-wide mathematical models are promising techniques. An integrated systems approach of these techniques drives diversity and quantity of several new biofuel compounds. This review will cover the recent technological developments that support improvement of the advanced biofuels 1-butanol, biodiesels and jetfuels.
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| 5. |
- Helariutta, Yrjö
(författare)
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Arabidopsis as a model for wood formation
- 2011
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 22, s. 293-299
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Forskningsöversikt (refereegranskat)abstract
- Wood (secondary xylem) is one of the most important sustainable energy sources for humans. Arabidopsis, despite its herbaceous nature, has become an excellent model to study wood formation. Recent progress has shown that conserved molecular mechanisms may exist in herbaceous plants and trees during vascular development and wood formation. Several transcription factor families and plant hormone species as well as other factors contribute to the regulation of xylem development in both Arabidopsis and woody plants. In this review, we highlight how information gained from the analysis of vascular development in Arabidopsis has improved our understanding of wood formation in trees.
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| 6. |
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| 7. |
- Korten, Till, et al.
(författare)
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Towards the application of cytoskeletal motor proteins in molecular detection and diagnostic devices
- 2010
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 21:4, s. 477-488
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Tidskriftsartikel (refereegranskat)abstract
- Over the past ten years, great advancements have been made towards using biomolecular motors for nanotechnological applications. In particular, devices using cytoskeletal motor proteins for molecular transport are maturing. First efforts towards designing such devices used motor proteins attached to micro-structured substrates for the directed transport of microtubules and actin filaments. Soon thereafter, the specific capture, transport and detection of target analytes like viruses were demonstrated. Recently, spatial guiding of the gliding filaments was added to increase the sensitivity of detection and allow parallelization. Whereas molecular motor powered devices have not yet demonstrated performance beyond the level of existing detection techniques, the potential is great: Replacing microfluidics with transport powered by molecular motors allows integration of the energy source (ATP) into the assay solution. This opens up the opportunity to design highly integrated, miniaturized, autonomous detection devices. Such devices, in turn, may allow fast and cheap on-site diagnosis of diseases and detection of environmental pathogens and toxins.
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| 8. |
- Löfblom, John, et al.
(författare)
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Non-immunoglobulin based protein scaffolds
- 2011
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 22:6, s. 843-848
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Forskningsöversikt (refereegranskat)abstract
- Non-immunoglobulin based protein scaffolds have been reported as promising alternatives to traditional monoclonal antibodies for over a decade and are often mentioned as part of the next-generation immunotherapeutics. Today, this class of biologics is beginning to demonstrate its potential for therapeutic applications and several are currently in preclinical or clinical development. A common denominator for most of these new scaffolds is the attractive properties that differentiate them from monoclonal antibodies including small size, cysteine-free sequence, flexible pharmacokinetic properties, and ease of generating multispecific molecules. In addition to therapeutic applications, substantial evidence point to superior performance of several of these scaffolds in molecular imaging compared to full-length antibodies. Here we review the most recent progress using alternative protein scaffolds for therapy and medical imaging.
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| 9. |
- Marques, Tatiana Milena, et al.
(författare)
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Programming infant gut microbiota : influence of dietary and environmental factors
- 2010
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Ingår i: Current Opinion in Biotechnology. - Oxford, United Kingdom : Elsevier. - 0958-1669 .- 1879-0429. ; 21:2, s. 149-156
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Forskningsöversikt (refereegranskat)abstract
- The neonatal period is crucial for intestinal colonisation, and the composition of this ecosystem in early life is influenced by such factors as mode of birth, environment, diet and antibiotics. The intestinal microbiota contributes to protection against pathogens, maturation of the immune system and metabolic welfare of the host, but under some circumstances can contribute to the pathogenesis of certain diseases. Because colonisation with non-pathogenic microbiota is important for infant health and may affect health in later life, it is important to understand how the composition of this microbial organ is established and by which dietary means (e.g. supplementation with prebiotics/probiotics/food ingredients) it can be programmed in order to achieve an ecosystem that is valuable for the host.
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| 10. |
- Martinez Ruiz, Jose Luis, 1981, et al.
(författare)
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Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation
- 2012
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 23:6, s. 965-971
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Forskningsöversikt (refereegranskat)abstract
- Since the approval of recombinant insulin from Escherichia coli for its clinical use in the early 1980s, the amount of recombinant pharmaceutical proteins obtained by microbial fermentations has significantly increased. The recent advances in genomics together with high throughput analysis techniques (the so-called - omics approaches) and integrative approaches (systems biology) allow the development of novel microbial cell factories as valuable platforms for large scale production of therapeutic proteins. This review summarizes the main achievements and the current situation in the field of recombinant therapeutics using yeast Saccharomyces cerevisiae as a model platform, and discusses the future potential of this platform for production of blood proteins and substitutes.
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