| 1. |
- Reczey, K, et al.
(författare)
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Cellulase production by T-reesei
- 1996
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Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 57:1, s. 25-30
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Tidskriftsartikel (refereegranskat)abstract
- Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd.
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| 2. |
- von Sivers, M, et al.
(författare)
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Ethanol from lignocellulosics: A review of the economy
- 1996
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Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 56:2-3, s. 131-140
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Tidskriftsartikel (refereegranskat)abstract
- The present study is a review of published investigations regarding the economy of ethanol production from lignocellulosic material The objective is to present relations between and tendencies observed in different cost estimates. The influence of plant capacity and overall product yield on the ethanol production cost is investigated, as well as variations in capital costs in the different processes. The underlying technical and economic assumptions show a large variation between the various studies published. The variation in the ethanol production cost is large, from 18 to 151 US cent/l. The most important factor for the economic outcome is the overall ethanol yield. Other important parameters are the feedstock cost, which was found to vary between 22 and 61 US$/dry metric ton, and the plant capacity, which influences the capital cost. It is shown that there is a tendency towards a decrease in ethanol production cost with an increase in plant capacity for the enzymatic processes. A high yield also results in a decrease in production cost for the enzymatic and dilute acid processes in the papers reviewed. Copyright (C) 1996 Elsevier Science Ltd.
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| 3. |
- Christakopoulos, Paul, et al.
(författare)
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Production and partial characterization of xylanase from Fusarium oxysporum
- 1996
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Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 58:2, s. 115-119
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Tidskriftsartikel (refereegranskat)abstract
- Production of xylanase by Fusarium oxysporum strain F3 was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source, and the growth temperature. Under these conditions, yields as high as 245 U/ml of culture medium were obtained. The most important characteristic of the enzyme is its high pH stability. It retained 80 and 66% of the activity at pH 9.0 after 24 h at 4 and 30°C, respectively. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major, one minor xylanase and one active β-xylosidase exhibiting pI values of 9.5, 6.5 and 3.8, respectively
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| 4. |
- Taherzadeh, Mohammad J, 1965-, et al.
(författare)
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Conversion of dilute-acid hydrolyzates of spruce and birch to ethanol by fed-batch fermentation
- 1999
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Ingår i: Bioresource Technology. - Exeter, United Kingdom : Elsevier Sci Ltd. - 0960-8524 .- 1873-2976. ; 69:1, s. 59-66
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Tidskriftsartikel (refereegranskat)abstract
- Fermentation techniques for conversion of dilute acid hydrolyzates were examined. Batch and fed-batch fermentations of hydrolyzates from spruce and birch woods were made in a lab-scale (3.31) anaerobic bioreactor using the yeast Saccharomyces cerevisiae. The spruce and birch hydrolyzates contained high initial concentrations of furfural (2.2 and 5.7 g/l) and 5-hydroxymethylfurfural (HMF, 7.3 and 2.4 g/l), and were found to be strongly inhibiting to the yeast strain used in this study. Fermentation of the hydrolyzates was not possible using a batch mode of operation. However, using a fed-batch technique with a suitably adjusted feed rate, it was found possible to completely ferment the glucose and mannose sugars in both hydrolyzates. Most of the furfural (90%), and part of the HMF (40-70%), present in the hydrolyzates was converted during the fed-batch operation. It is suggested that the success of the fed-batch operation is related to the conversion of furfural and HMF.Fermentation techniques for conversion of dilute acid hydrolyzates were examined. Batch and fed-batch fermentations of hydrolyzates from spruce and birch woods were made in a lab-scale (3.31) anaerobic bioreactor using the yeast Saccharomyces cerevisiae. The spruce and birch hydrolyzates contained high initial concentrations of furfural (2.2 and 5.7 g/l) and 5-hydroxymethylfurfural (HMF, 7.3 and 2.4 g/l), and were found to be strongly inhibiting to the yeast strain used in this study. Fermentation of the hydrolyzates was not possible using a batch mode of operation. However, using a fed-batch technique with a suitably adjusted feed rate, it was found possible to completely ferment the glucose and mannose sugars in both hydrolyzates. Most of the furfural (90%), and part of the HMF (40-70%), present in the hydrolyzates was converted during the fed-batch operation. It is suggested that the success of the fed-batch operation is related to the conversion of furfural and HMF.
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