| 1. |
- Zachary, Ian, et al.
(författare)
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Inhibition of bombesin-induced mitogenesis by pertussis toxin : Dissociation from phospholipase C pathway.
- 1987
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Academic Press. - 0006-291X .- 1090-2104. ; 146:2, s. 456-463
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Tidskriftsartikel (refereegranskat)abstract
- Prior incubation of quiescent cultures of Swiss 3T3 cells with pertussis toxin selectively inhibited the stimulation of DNA synthesis induced by peptides of the bombesin family. While pertussis toxin blocked mitogenesis at an early stage in the action of the peptide, the toxin did not impair the rapid stimulation of polyphosphoinositide breakdown, Ca2+ mobilization or activation of protein kinase C promoted by bombesin. Thus, inhibition of bombesin-induced mitogenesis by pertussis toxin can be dissociated from inactivation of the phospholipase C signalling pathway.
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| 2. |
- Ali, L, et al.
(författare)
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Free and bound sodium in pancreatic beta-cells exposed to glucose and tolbutamide.
- 1989
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 164:1, s. 212-8
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Tidskriftsartikel (refereegranskat)abstract
- The effects of glucose and tolbutamide on the sodium handling of the pancreatic beta-cells were evaluated by measuring the total sodium content in intact islets from ob/ob-mice by integrating flame photometry and the free ion in individual beta-cells by dual wavelength fluorometry. Whereas increasing the glucose concentration from 3 to 20 mM resulted in a lowering of sodium, the addition of 100 microM tolbutamide caused a rise. The above-mentioned effects were most marked (about 50%) for the physiologically significant free sodium. The data indicate a more important role for Na+ in the regulation of insulin release than so far acknowledged. Increase of Na+ may contribute to the secretory response to hypoglycemic sulfonylureas by providing an additional rise of cytoplasmic Ca2+.
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| 3. |
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| 4. |
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| 5. |
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| 6. |
- Spasokukotskaja, T., et al.
(författare)
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Deoxycytidine is salvaged not only into DNA but also into phospholipid precursors
- 1988
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier. - 0006-291X .- 1090-2104. ; 155:2, s. 923-929
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Tidskriftsartikel (refereegranskat)abstract
- Deoxycytidine metabolism was investigated in light density human tonsillar lymphocytes using 5-3H-deoxycytidine as extracellular precursor. A significant portion of the deoxycytidine (more than 50% of ethanol soluble pool) was found to incorporate into dCDP-choline and dCDP-ethanolamine beside the well-known pathway i.e. incorporation into DNA in form of dCMP and dTMP. Hydroxyurea increased the labeling of the deoxyliponucleotides from 5-3H-deoxycytidine in spite of its inhibition of DNA synthesis.
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| 7. |
- Spyrou, Giannis, et al.
(författare)
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Ribonucleotides are not channeled into DNA in permeabilized mammalian cells
- 1983
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - : Elsevier. - 0006-291X .- 1090-2104. ; 115:3, s. 1022-1026
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Tidskriftsartikel (refereegranskat)abstract
- Reddy and Pardee earlier reported that permeabilized CHEF/18 cells (J. Biol. Chem 257, 12526) or soluble extracts from such cells (Cell 32, 443) under the proper conditions preferentially incorporated a labeled ribonucleotide (CDP) into DNA without dilution by an excess of non-labeled dCTP, suggesting channeling of ribonucleotides into DNA by a multiprotein complex. Here we present results suggesting that in these experiments CDP was incorporated into RNA and not into DNA.
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| 8. |
- Söderström, M, et al.
(författare)
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Leukotriene C4 formation catalyzed by three distinct forms of human cytosolic glutathione transferase.
- 1985
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Ingår i: Biochemical and Biophysical Research Communications - BBRC. - 0006-291X .- 1090-2104. ; 128:1, s. 265-70
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Tidskriftsartikel (refereegranskat)abstract
- The ability of three distinct types of human cytosolic glutathione transferase to catalyze the formation of leukotriene C4 from glutathione and leukotriene A4 has been demonstrated. The near-neutral transferase (mu) was the most efficient enzyme with Vmax= 180 nmol X min-1 X mg-1 and Km= 160 microM. The Vmax and Km values for the basic (alpha-epsilon) and the acidic (pi) transferases were 66 and 24 nmol X min-1 X mg-1 and 130 and 190 microM, respectively. The synthetic methyl ester derivative of leukotriene A4 was somewhat more active as a substrate for all the three forms of the enzyme.
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| 9. |
- Escribano, Julio, et al.
(författare)
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Identification of retinol as one of the protein HC chromophores
- 1988
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Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 0006-291X. ; 155:3, s. 1424-1429
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Tidskriftsartikel (refereegranskat)abstract
- Protein HC (alias alpha 1-microglobulin) contains so far unidentified yellow-brown fluorescent chromophores. Several preparations of human protein HC were extracted with hexane. Most of the extracts contained a substance which, upon reversed-phase HPLC, co-eluted with all-trans- retinol and had an absorption spectrum identical to that of retinol. The substance was also, like retinol, destroyed by exposure to ultraviolet light and acid pH. These observations strongly support the proposal that protein HC is a member of the newly defined lipocalin protein superfamily. The highest retinol-protein HC molar ratio of the investigated protein HC preparations was 1.6 x 10(-3) indicating that retinol is not the only ligand bound to protein HC. This was confirmed by comparing the absorption spectrum of protein HC before and after hexane extraction.
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| 10. |
- López-Otin, C, et al.
(författare)
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Neutral microprotein, a novel human plasma and urinary protein associated with a yellow-brown chromophore. Isolation from protein HC preparations and partial characterization
- 1983
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Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 0006-291X. ; 117:1, s. 9-202
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Tidskriftsartikel (refereegranskat)abstract
- An apparently novel human plasma and urinary protein of low molecular weight was isolated from several highly purified preparations of protein HC by gel chromatography and high voltage electrophoresis with a yield of about 8 mg/g. The protein has a molecular weight of about 20,000, neutral electrophoretic mobility at pH 6.5 and a high content of half-cystine. It is associated with a yellow-brown chromophore like protein HC and could be demonstrated in all investigated preparations of isolated human, rabbit and guinea-pig protein HC and alpha 1-microglobulin.
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