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- Brodelius, Peter, et al.
(author)
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Determination of Dissociation Constants for Binary Dehydrogenase-Coenzyme Complexes by (Bio)Affinity Chromatography on an Immobilized AMP-Analogue
- 1976
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In: Analytical biochemistry. - : Elsevier BV. - 0003-2697. ; 72:1-2, s. 629-636
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Journal article (peer-reviewed)abstract
- Various alcohol and lactate dehydrogenases were adsorbed to an affinity column of immobilized N6-(6-aminohexyl)-AMP and subsequently eluted with gradients of coenzymes or coenzyme fragments. A linear relation was observed between the eluting concentration of nucleotide and the reported dissociation constants for the corresponding binary enzyme-nucleotide complexes. This relation has been utilized to determine unknown dissociation constants by affinity chromatography. The method presented can also be utilized for the estimation of dissociation constants betweendehydrogenases and coenzyme analogues.
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- Petersson, Göran, 1941
(author)
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Conversion of dehydroascorbic acid to a branched hexaric acid in neutral and alkaline aqueous solution
- 1976
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In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697. ; 72, s. 623-628
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Journal article (peer-reviewed)abstract
- Dehydroascorbic acid is shown to be converted to 2-(threo-1,2,3-trihydroxypropyl)tartronic acid in aqueous alkaline solutions. The structure of the acid was determined by mass spectrometry of its acyclic Me3Si derivative. Mass spectrometric and chromatographic data are compared with those of related compounds. The acid is formed by a benzilic acid rearrangement of the intermediate 2,3-hexodiulosonic acid. The rate of formation at 38°C was studied quantitatively by glc. It increases at increased alkalinity but is significant even at physiological pH. The presence of oxygen does not substantially influence the reaction.
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