| 1. |
- Bäck, Rune, et al.
(författare)
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Influence of Antigenic Stimulation on Lymphoid Cell Traffic in the Chicken : I. Increased Homing of Thymus-Derived Cells to the Bone Marrow after Antigenic Stimulation
- 1972
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Ingår i: International Archives of Allergy and Applied Immunology. - : S. Karger AG. - 0020-5915 .- 1018-2438 .- 1423-0097. ; 43:5, s. 657-670
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Tidskriftsartikel (refereegranskat)abstract
- Six-week-old chickens were either immunized with a single intravenous injection of human serum albumin (HSA) or injected intravenously with physiological saline. 24 h after the HSA or saline administration the chickens were locally labelled in the thymus with 3H-thymidine in vivo. 48 h after the intrathymic labelling the chickens were sacrificed and the influence of the antigenic stimulation on the distribution of label between different lymphoid organs was studied with a radiochemical method based on measurements of tritiated DNA, and with autoradiography. A significantly increased proportion of thymus-derived label was demonstrated with the radiochemical method in the bone marrow of the immunized chickens, as compared to the saline-injected controls. With autoradiography, an increased number of heavily labelled thymus-derived cells were found in the bone marrow of the HSA-immunized chickens. The majority of these cells were small to medium-sized lymphoid cells, but a number of them were large lymphoid cells. None of these cells were plasma cells. There was no apparent increase of thymus-derived label or heavily labelled thymus-derived cells in the spleen or in the cecal tonsils of the immunized chickens. Heavily labelled thymus-derived cells could not be demonstrated in the bursa of Fabricius.
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| 2. |
- Bäck, Rune
(författare)
-
Influence of Antigenic Stimulation on Lymphoid Cell Traffic in the Chicken : II. Increased Homing of Bone Marrow-Derived Cells to the Bursa of Fabricius after Human Serum Albumin Administration
- 1972
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Ingår i: International Archives of Allergy and Applied Immunology. - : S. Karger AG. - 0020-5915 .- 1018-2438 .- 1423-0097. ; 43:6, s. 921-933
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Tidskriftsartikel (refereegranskat)abstract
- 6-week-old chickens were immunized with a single intravenous injection of human serum albumin (HSA). 24 h after the immunization, the chickens and nonimmunized saline-injected control chickens were locally labelled in the bone marrow with 3H-thymidíne. 48 h after labelling, the chickens were sacrificed and the distribution of label between different lymphoid organs in the immunized and the nonimmunized chickens was compared with a radiochemical method and with autoradiography. An increased proportion of bone marrow-derived tritium-labelled DNA and significantly more heavily labelled bone marrow-derived cells were found in the bursa of Fabricius of the HSA-immunized chickens as compared with the nonimmunized controls. The heavily labelled bone marrow-derived cells were pyroninophilic, and were located in the follicular cortex, as well as in the medulla. There was no indication of an increase of the normal transport of label or of heavily labelled cells from the bone marrow to other lymphoid organs in the immunized chickens. It is suggested that the increased homing of bone marrow-derived cells to the bursa of Fabricius, 72 h after the antigenic stimulation, may reflect a recruitment of bursa cell precursors.
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| 3. |
- Foucard, Tony, et al.
(författare)
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In vitro Estimation of Allergens by a Radioimmune Antiglobulin Technique Using Human IgE Antibodies
- 1972
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Ingår i: International Archives of Allergy and Applied Immunology. - : S. Karger AG. - 0020-5915 .- 1018-2438 .- 1423-0097. ; 43:3, s. 360-370
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Tidskriftsartikel (refereegranskat)abstract
- A radioimmune method based on detection of IgE antibodies (RAST) has been used for allergen quantitation, both as a direct test and as an inhibition test. Commercially available allergen extracts from birch pollen, timothy pollen and horse dandruff were studied. Variations of the allergen concentration up to a thousandfold were found between some extracts and these differences were confirmed by the results obtained with the chopped human lung test and in provocation tests. The use of RAST for estimation of allergen concentrations seems to be of great value, since the method combines easy performance with high capacity and independence of allergic patients as test objects. The same set of test sera can be used for several years, ensuring standardized conditions.
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| 4. |
- Hemmingsson, Erik J.
(författare)
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Ontogenetic Studies on Lymphoid Cell Traffic in the Chicken : III. Cell Traffic from the Thymus
- 1972
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Ingår i: International Archives of Allergy and Applied Immunology. - : S. Karger AG. - 0020-5915 .- 1018-2438 .- 1423-0097. ; 43:4, s. 481-496
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Tidskriftsartikel (refereegranskat)abstract
- Male chickens of different ages were injected with tritiated thymidine into the parenchyma of the thymus and the transport of thymic label and of thymus-derived cells to other lymphoid organs was studied. Using a radiochemical method based on DNA and tritium measurements in different organs, a quantitatively significant transport of label from the thymus to the spleen, the caecal tonsils, and to the bone marrow could be demonstrated in the 9-day-old chicken. The transport to the spleen was shown to continue in the 6-week-old and 14-week-old animal. Autoradiographically, thymus-derived cells could be found in the spleen and caecal tonsils as early as in the newly hatched period. Autoradiographic data also indicated that the traffic increased up to 6 weeks of age but decreased in older animals. In the 6-week-old group, the localization of the thymus-derived cells in the target organs was determined. Such cells in the spleen were distributed almost equally between the red and white pulp. Very few of the thymus-derived cells were highly pyroninophilic. None could be found in the germinal centres. No sign of cell transport from the thymus to the bursa of Fabricius could be detected. The pattern of cell migration is well correlated to the morphological and functional maturation of the lymphoid system.
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