| 1. |
- Aboulaich, Nabila, et al.
(författare)
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Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes
- 2004
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Ingår i: The Biochemical journal. - 1470-8728. ; 383:Pt 2, s. 237-248
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Tidskriftsartikel (refereegranskat)abstract
- Caveolae, the specialized invaginations of plasma membranes, formed sealed vesicles with outwards-orientated cytosolic surface after isolation from primary human adipocytes. This morphology allowed differential, vectorial identification of proteins at the opposite membrane surfaces by proteolysis and MS. Extracellular-exposed caveolae-specific proteins CD36 and copper-containing amine oxidase were concealed inside the vesicles and resisted trypsin treatment. The cytosol-orientated caveolins were efficiently digested by trypsin, producing peptides amenable to direct MS sequencing. Isolation of peripheral proteins associated with the cytosolic surface of caveolae revealed a set of proteins that contained nuclear localization signals, leucine-zipper domains and PEST (amino acid sequence enriched in proline, glutamic acid, serine and threonine) domains implicated in regulation by proteolysis. In particular, PTRF (polymerase I and transcript release factor) was found as a major caveolae-associated protein and its co-localization with caveolin was confirmed by immunofluorescence confocal microscopy. PTRF was present at the surface of caveolae in the intact form and in five different truncated forms. Peptides (44 and 45 amino acids long) comprising both the PEST domains were sequenced by nanospray-quadrupole-time-of-flight MS from the full-length PTRF, but were not found in the truncated forms of the protein. Two endogenous cleavage sites corresponding to calpain specificity were identified in PTRF; one of them was in a PEST domain. Both cleavage sites were flanked by mono- or diphosphorylated sequences. The phosphorylation sites were localized to Ser-36, Ser-40, Ser-365 and Ser-366 in PTRF. Caveolae of human adipocytes are proposed to function in targeting, relocation and proteolytic control of PTRF and other PEST-domain-containing signalling proteins.
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| 2. |
- Abrahamson, Magnus, et al.
(författare)
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Structure and expression of the human cystatin C gene
- 1990
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Ingår i: Biochemical Journal. - 1470-8728. ; 268:2, s. 287-294
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Tidskriftsartikel (refereegranskat)abstract
- The structural organization of the gene for the human cysteine-proteinase inhibitor cystatin C was studied. Restriction-endonuclease digests of human genomic DNA hybridized with human cystatin C cDNA and genomic probes produced patterns consistent with a single cystatin C gene and, also, the presence of six closely related sequences in the human genome. A 30 kb restriction map covering the genomic region of the cystatin C gene was constructed. The positions of three polymorphic restriction sites, found at examination of digests of genomic DNA from 79 subjects, were localized in the flanking regions of the gene. The gene was cloned and the nucleotide sequence of a 7.3 kb genomic segment was determined, containing the three exons of the cystatin C structural gene as well as 1.0 kb of 5'-flanking and 2.0 kb of 3'-flanking sequences. Northern-blot experiments revealed that the cystatin C gene is expressed in every human tissue examined, including kidney, liver, pancreas, intestine, stomach, antrum, lung and placenta. The highest cystatin C expression was seen in seminal vesicles. The apparently non-tissue-specific expression of this cysteine-proteinase inhibitor gene is discussed with respect to the structure of its 5'-flanking region, which shares several features with those of housekeeping genes.
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| 3. |
- Aguilo, Francesca, et al.
(författare)
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THAP1 : role in mouse embryonic stem cell survival and differentiation
- 2017
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Ingår i: Stem Cell Reports. - : Cell Press. - 2213-6711. ; 9:1, s. 92-107
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Tidskriftsartikel (refereegranskat)abstract
- THAP1 (THAP [Thanatos-associated protein] domain-containing, apoptosis-associated protein 1) is a ubiquitously expressed member of a family of transcription factors with highly conserved DNA-binding and protein-interacting regions. Mutations in THAP1 cause dystonia, DYT6, a neurologic movement disorder. THAP1 downstream targets and the mechanism via which it causes dystonia are largely unknown. Here, we show that wild-type THAP1 regulates embryonic stem cell (ESC) potential, survival, and proliferation. Our findings identify THAP1 as an essential factor underlying mouse ESC survival and to some extent, differentiation, particularly neuroectodermal. Loss of THAP1 or replacement with a disease-causing mutation results in an enhanced rate of cell death, prolongs Nanog, Prdm14, and/or Rex1 expression upon differentiation, and results in failure to upregulate ectodermal genes. ChIP-Seq reveals that these activities are likely due in part to indirect regulation of gene expression.
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| 4. |
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| 5. |
- Anttu, Nicklas, et al.
(författare)
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Reflection measurements to reveal the absorption in nanowire arrays
- 2013
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Ingår i: Optics Letters. - 0146-9592. ; 38:9, s. 1449-1451
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Tidskriftsartikel (refereegranskat)abstract
- The absorption of light is at the core of photovoltaic applications. For many nanostructure-based devices, an assessment of the absorption in the nanostructures is complicated by a thick, opaque substrate that prohibits transmission measurements. Here, we show how a single reflection measurement can be used for approximating the amount of light absorbed in vertical semiconductor nanowire arrays. (C) 2013 Optical Society of America
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| 6. |
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| 7. |
- Arentzen, Thomas, docent, 1976-, et al.
(författare)
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Wisdom on the Move : An Introduction
- 2020
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Ingår i: Wisdom on the Move: Late Antique Traditions in Multicultural Conversation. - Leiden : Brill Nijhoff. - 0920-623X. - 9789004430693 - 9789004430747 ; , s. 1-10
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Bokkapitel (refereegranskat)
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| 8. |
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| 9. |
- Baev, A, et al.
(författare)
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Picturing molecular femtosecond processes through an ultra-fast controllable X-ray shutter
- 2003
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Ingår i: Chemical Physics. - 0301-0104 .- 1873-4421. ; 289, s. 51-56
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Tidskriftsartikel (refereegranskat)abstract
- We show that frequency detuning in a resonant X-ray scattering experiment acts as an X-ray camera shutter by regulating the duration time of the scattering process. The camera shutter can be used to select processes at different time scales for observation. This is illustrated by a resonant Auger study of the ultra-fast dissociation of the core-excited HF molecule. We present experimental results and first principle simulations of the molecular fraction in the resonant Auger spectra of HF which is a dynamical parameter that well illustrates X-ray shutter controlled dissociation.
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| 10. |
- Baird, Julia, et al.
(författare)
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Introducing Resilience Practice to Watershed Groups : What Are the Learning Effects?
- 2016
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Ingår i: Society & Natural Resources. - : Informa UK Limited. - 0894-1920 .- 1521-0723. ; 29:10, s. 1214-1229
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Tidskriftsartikel (refereegranskat)abstract
- Resilience as an organizing framework for addressing dynamics of social-ecological systems has experienced strong uptake; however, its application is nascent. This research study aimed to address the gap between resilience thinking and practice by focusing on learning, a key aspect of resilience. Two Canadian watershed groups were led in 2-day workshops focused on resilience. Learning effects were measured using a survey administered both before and after the workshop, and a qualitative survey was administered 6 months later to understand longer term effects. Short-term learning effects were similar between the two case studies, with strong cognitive and relational learning and less normative learning. Longer term effects showed enduring cognitive and normative learning in both cases; however, relational learning persisted only in the watershed where a resilience practice approach to watershed planning had been incorporated. Future research directions include refinements to the learning measurement methodology and continuing to build resilience practice literature.
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