| 1. |
|
|
| 2. |
- Brånemark, Rickard, 1960, et al.
(författare)
-
Bone response to laser-induced micro- and nano-size titanium surface features.
- 2011
-
Ingår i: Nanomedicine : nanotechnology, biology, and medicine. - : Elsevier BV. - 1549-9642 .- 1549-9634.
-
Tidskriftsartikel (refereegranskat)abstract
- The present study explored whether laser-induced, site-specific implant surface modifications with micro- and nano-scale topography were able to promote bone formation. The aim was to evaluate the biomechanical and histological response to partly laser-modified titanium implants compared with machined implants. After an early 8-week healing period in rabbit tibia and femur, a 250% increase in removal torque was demonstrated for the partly laser-modified surface. Further, different fracture mechanisms were demonstrated for the two surfaces. Histologically, significantly more bone was found in direct contact with the laser-modified surface for the implants in the tibia sites, while a similar amount of bone tissue was observed in contact with the implant in the femoral sites. In conclusion, an improved bone-implant interface anchorage was promoted by an increase in micro- and nano-scale implant surface topography and surface oxide induced by topological laser treatment.
|
|
| 3. |
- Dell'Orco, Daniele, et al.
(författare)
-
Delivery success rate of engineered nanoparticles in the presence of the protein corona: a systems-level screening
- 2012
-
Ingår i: Nanomedicine: Nanotechnology, Biology and Medicine. - : Elsevier BV. - 1549-9642 .- 1549-9634. ; 8:8, s. 1271-1281
-
Tidskriftsartikel (refereegranskat)abstract
- Nanoparticles (NPs) for medical applications are often introduced into the body via intravenous injections, leading to the formation of a protein corona on their surface due to the interaction with blood plasma proteins. Depending on its composition and time evolution, the corona will modify the biological behavior of the particle. For successful delivery and targeting, it is therefore important to assess on a quantitative basis how and to what extent the presence of the corona perturbs the specific interaction of a designed NP with its cellular target. We present a theoretical systems-level analysis, in which peptides have been covalently coupled to the surface of nanoparticles, describing the delivery success rate in varying conditions, with regard to protein composition of the surrounding fluid. Dynamic modeling and parameter sensitivity analysis proved to be useful and computationally affordable tools to aid in the design of NPs with increased success rate probability in a biological context. FROM THE CLINICAL EDITOR: The formation of a protein corona consisting of blood plasma proteins on the surface of intravenously delivered nanoparticles may modify the biological behavior of the particles. This team of investigators present a theoretical systems-level analysis of this important and often neglected phenomenon.
|
|
| 4. |
- Jain, Achint K., et al.
(författare)
-
Targeting of diacerein loaded lipid nanoparticles to intra-articular cartilage using chondroitin sulfate as homing carrier for treatment of osteoarthritis in rats
- 2014
-
Ingår i: Nanomedicine. - : Elsevier BV. - 1549-9634 .- 1549-9642. ; 10:5, s. 1031-1040
-
Tidskriftsartikel (refereegranskat)abstract
- Targeted delivery of antiosteoarthritic drug diacerein to articular tissue could be a major achievement and soluble polysaccharide chondroitin sulfate (ChS) may be a suitable agent for this. Therefore, diacerein loaded solid lipid nanoparticles modified with ChS (ChS-DC-SLN) were prepared for synergistic effect of these agents to combat multidimensional pathology of osteoarthritis (OA). Prepared formulation were of size range 396. ±. 2.7. nm, showed extended release up to 16. h and increased bioavailability of diacerein by 2.8 times. ChS-DC-SLN were evaluated for their effect on histopathology of femoro-tibial joint of rat knee and amount of ChS and rhein (an active metabolite of diacerein) at targeted site. Concentration of rhein was significantly higher in case of ChS-DC-SLN (7.8. ±. 1.23. μg/ml) than that of drug dispersion (2.9. ±. 0.45. μg/ml). It can be stated that ChS served as homing to articular cartilage for targeting of drug. Thus, ChS-DC-SLN have great potential to enhance the overall efficacy of treatment for OA. From the Clinical Editor: This study demonstrates the feasibility of targeted delivery of diacerein to articular tissue using soluble polysaccharide chondroitin sulfate as the targeting vector. This approach has the potential to significantly increase anti-arthritic drug concentration in joints without leading to systemic toxicity
|
|
| 5. |
- Kjellman, Pontus, et al.
(författare)
-
Optimizing retention of multimodal imaging nanostructures in sentinel lymph nodes by nanoscale size tailoring.
- 2014
-
Ingår i: Nanomedicine: Nanotechnology, Biology and Medicine. - : Elsevier BV. - 1549-9642 .- 1549-9634. ; 10:5, s. 1089-1095
-
Tidskriftsartikel (refereegranskat)abstract
- This study investigates the retention of different sized ultra-small superparamagnetic iron oxide nanoparticles (USPIOs) in lymph nodes of healthy rats, after subcutaneous injection. Three distinct sizes (15, 27 and 58 nm) of USPIOs were synthesized by only varying the thickness of the polymer coating surrounding the 10 nm cores. Particles were injected on the dorsal side of the hind paw of rats and the uptake in the popliteal, inguinal and iliac lymph nodes was monitored. The data reveal that the 15 nm particle accumulates more rapidly and to a higher amount in the first lymph node than the two larger particles. A clear contrast between the first and second lymph nodes could be detected indicating that even the rather small difference in particle size (15-58 nm) tested has significant effects on the retention of USPIOs in the lymph nodes.
|
|
| 6. |
|
|
| 7. |
- Oslakovic, Cecilia, et al.
(författare)
-
Polystyrene nanoparticles affecting blood coagulation.
- 2012
-
Ingår i: Nanomedicine: Nanotechnology, Biology and Medicine. - : Elsevier BV. - 1549-9642 .- 1549-9634. ; 8:6, s. 981-986
-
Tidskriftsartikel (refereegranskat)abstract
- Association of nanoparticles with blood coagulation proteins may influence the natural balance between pro-and anticoagulant pathways. We investigated whether polystyrene nanoparticles, when added to human plasma, affected the generation of thrombin in plasma. Amine-modified nanoparticles were found to decrease the thrombin formation due to binding of factor VII and IX to the nanoparticles, which resulted in depletion of the respective protein in solution. In contrast, carboxyl-modified nanoparticles were able to act as a surface for activation of the intrinsic pathway of blood coagulation in plasma. These results highlight the influence of nanoparticles on a biologically important pathway.
|
|
| 8. |
- Patra, Hirak, 1981-, et al.
(författare)
-
Cancer cell response to nanoparticles : criticality and optimality
- 2012
-
Ingår i: Nanomedicine. - : Elsevier. - 1549-9634 .- 1549-9642. ; 8:6, s. 841-852
-
Tidskriftsartikel (refereegranskat)abstract
- A stochastic variation in size and electrical parameters is common in nanoparticles. Synthesizing gold nanoparticles with a varying range of size and zeta potential, we show that there is clustering at certain regions of hydrodynamic diameter and zeta potentials that can be classified using k-clustering technique. A cluster boundary was observed around 50 nm, a size known for its optimal response to cells. However, neither size nor zeta potential alone determined the optimal cellular response (e.g., percentage cell survival) induced by such nanoparticles. A complex interplay prevails between size, zeta potential, nature of surface functionalization, and extent of adhesion of the cell to a solid matrix. However, it follows that the ratio of zeta potential to surface area, which scales as the electrical field (by Gaussian law), serves as an appropriate indicator for optimal cellular response. The phase plot spanned by fractional survival and effective electric field (charge density) indicates a positive correlation between mean cell survival and the magnitude of the electric field. The phase plot spanned by fractional survival and effective electric field (charge density) associated with the nanosurface shows a bifurcation behavior. Wide variation of cell survival response is observed at certain critical values of the surface charge density, whereas in other ranges the cellular response is well behaved and more predictable. Existence of phase points near the critical region corresponds to wide fluctuation of nanoparticle-induced response, for small changes in the nanosurface property. Smaller nanoparticles with low zeta potential (e.g., those conjugated with arginine) can have such an attribute (i.e., higher electrical field strength), and eventually they cause more cell death. The study may help in optimal design of nanodrugs.
|
|
| 9. |
- Roller, Jonas, et al.
(författare)
-
How to detect a dwarf - in vivo imaging of nanoparticles in the lung.
- 2011
-
Ingår i: Nanomedicine: Nanotechnology, Biology and Medicine. - : Elsevier BV. - 1549-9642 .- 1549-9634. ; 7:6, s. 753-762
-
Tidskriftsartikel (refereegranskat)abstract
- Nanotechnology is a rapidly developing field in science and industry. The exposure to nanoparticles (NPs) will steadily grow in the future and, thus, there is an urgent need to study potential impacts of the interaction between NPs and the human body. The respiratory tract is the route of entry for all accidentally inhaled NPs. Moreover, NPs may intentionally be delivered into the lung as contrast agents and drug delivery systems. The present review provides an overview of currently used techniques for the in vivo imaging of NPs in the lung, including X-ray, computed tomography (CT), gamma camera imaging, positron emission tomography (PET), magnetic resonance imaging (MRI), near-infrared imaging and intravital fluorescence microscopy. Studies based on these techniques may contribute to the development of novel NP-based drug delivery systems and contrast agents. In addition, they may provide completely new insights into nanotoxicological processes.
|
|
| 10. |
- Shah, Furqan A., et al.
(författare)
-
The bone-implant interface - nanoscale analysis of clinically retrieved dental implants
- 2014
-
Ingår i: Nanomedicine : nanotechnology, biology, and medicine. - : Elsevier BV. - 1549-9642 .- 1549-9634. ; 10:8, s. 1729-1737
-
Tidskriftsartikel (refereegranskat)abstract
- Evaluation of the fine structure of the bone-implant interface in humans is a prerequisite for a deepened understanding of structure-function relationships with nano-modified biomaterials. In this study, three clinically stable, yet retrieved, laser-modified dental implants were evaluated using histological and interface ultrastructural analyses. The cumulative results for all threads containing intact tissue showed remodeled Haversian bone with bone area and bone-implant contact in excess of 85% and 80%, respectively. Collagen fibrils, laid down parallel to the surface oxide layer, were mineralized by plate-like crystallites of stoichiometrically relevant (Ca/P ratios 1.30-1.67) bone-apatite. An overlap of titanium, oxygen, calcium and phosphorus signals indicated the gradual intermixing of bone-apatite and the nano-rough surface oxide. These results suggest that bone bonding to nano-textured titanium implant surfaces is promoted in human jaw-bone after functional loading.
|
|
