| 1. |
- Bruce, Benjamin, et al.
(författare)
-
Expression of the cytoskeleton linker protein ezrin in human cancers
- 2007
-
Ingår i: Clinical and Experimental Metastasis. - : Springer Science and Business Media LLC. - 1573-7276 .- 0262-0898. ; 24:2, s. 69-78
-
Tidskriftsartikel (refereegranskat)abstract
- Expression of the metastasis-associated protein, ezrin, in over 5,000 human cancers and normal tissues was analyzed using tissue microarray immunohistochemistry. Ezrin staining was compared between cancers and their corresponding normal tissues, between cancers of epithelial and mesenchymal origin, in the context of the putative inhibitor protein, merlin, and against clinicopathological data available for breast, lung, prostate cancers and sarcomas. Ezrin was found in most cancers and normal tissues at varying levels of intensity. In general ezrin was expressed at higher levels in sarcomas than in carcinomas. By normalizing the expression of ezrin in each cancer using ezrin expression found in the corresponding normal tissue, significant associations between ezrin were found in advancing histological grade in sarcomas (P = 0.02) and poor outcome in breast cancer (P = 0.025). Clinicopathologic associations were not changed by simultaneous assessment of ezrin and merlin in each patient sample for the cancer types examined. These data support a role for ezrin in the biology of human cancers and the need for additional studies in breast cancer and sarcoma patients that may validate ezrin as a marker of cancer progression and as a potential target for cancer therapy.
|
|
| 2. |
- Khrennikov, Andrei, et al.
(författare)
-
Kolmogorov probability spaces describing Accardi models for quantum correlations
- 2005
-
Ingår i: Open systems & information dynamics. - : World Scientific Pub Co Pte Lt. - 1230-1612 .- 1573-1324 .- 1793-7191. ; 12:4, s. 371-384
-
Tidskriftsartikel (refereegranskat)abstract
- In this paper we consider several Kolmogorov type models describing some quantum correlations. The main ideas beyond most of these models comes back to pioneering works of Accardi (see [1, 10, 15] and references therein); because of that we call some of them the Accardi models. Namely we discuss, in our frame, the Kolmogorov type Accardi model of the Aspect version [2] of the Einstein-Podolsky-Rosen (EPR) experiment [3].
|
|
| 3. |
- Nordstrand, Annika, et al.
(författare)
-
Establishment and validation of an in vitro co-culture model to study the interactions between bone and prostate cancer cells
- 2009
-
Ingår i: Clinical & experimental metastasis. - : Springer Science and Business Media LLC. - 0262-0898 .- 1573-7276. ; 26:8, s. 945-953
-
Tidskriftsartikel (refereegranskat)abstract
- Bone is the preferred site for prostate cancer (PCa) metastases. Once the tumor has established itself within the bone there is virtually no cure. To better understand the interactions between the PCa cells and bone environment in the metastatic process new model systems are needed. We have established a two-compartment in vitro co-culturing model that can be used to follow the trans-activation of bone and/or tumor cells. The model was validated using two PCa tumor cell lines (PC-3; lytic and LNCaP; mixed/osteoblastic) and one osteolytic inducing factor, 1,25-dihydroxyvitamin D(3) (D3). Results were in accordance with the expected bone phenotypes; PC-3 cells and D3 gave osteolytic gene expression profiles in calvariae, with up-regulation of genes needed for osteoclast differentiation, activation and function; Rankl, CathK, Trap and MMP-9, and down-regulation of genes associated with osteoblast differentiation and bone mineralization; Alp, Ocl and Dkk-1. LNCaP cells activated genes in the calvarial bones associated with osteoblast differentiation and mineralization, with marginal effects on osteolytic genes. The results were strengthened by similar changes in protein expression for a selection of the analyzed genes. Furthermore, the osteolytic gene expression profiles in calvarial bones co-cultured with PC-3 cells or with D3 were correlated with the actual ongoing resorptive process, as assessed by the release of collagen fragments from the calvariae. Our results show that the model can be used to follow tumor-induced bone remodeling, and by measuring changes in gene expression in the tumor cells we can also study how they respond to the bone microenvironment.
|
|
