| 1. |
- Aili, Daniel, 1977-, et al.
(författare)
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Colorimetric Protein Sensing by Controlled Assembly of Gold Nanoparticles Functionalized with Synthetic Receptors
- 2009
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Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 5:21, s. 2445-2452
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Tidskriftsartikel (refereegranskat)abstract
- A novel strategy is described for the colorimetric sensing of proteins, based on polypeptide-functionalized gold nanoparticles. Recognition is accomplished using a polypeptide sensor scaffold designed to specifically bind to the model analyte, human carbonic anhydrase II (HCAII). The extent of particle aggregation, induced by the Zn2+-triggered dimerization and folding of a second polypeptide also present on the surface of the gold nanoparticle, gives a readily detectable colorimetric shift that is dependent on the concentration of the target protein. In the absence of HCAII, particle aggregation results in a major redshift of the plasmon peak, whereas analyte binding prevented the formation of dense aggregates, significantly reducing the magnitude of the redshift. The versatility of the technique is demonstrated using a second model system based on the recognition of a peptide sequence from the tobacco mosaic virus coat protein (TMVP) by a recombinant antibody fragment (Fab57P). Concentrations down to approximate to 10 nM and approximate to 25 nM are detected for HCAII and Fab57P, respectively. This strategy is proposed as a generic platform for robust and specific protein analysis that can be further developed to monitor a wide range of target proteins.
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| 2. |
- Aili, Daniel, et al.
(författare)
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Colorimetric sensing: Small 21/2009
- 2009
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Ingår i: Small. - : John Wiley & Sons. - 1613-6810 .- 1613-6829. ; 5:21
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The cover picture illustrates a novel concept for colorimetric protein sensing based on the controllable assembly of polypeptide-functionalized gold nanoparticles. Recognition of the analyte is accomplished by polypeptide-based synthetic receptors immobilized on gold nanoparticles. Also present on the particle surface is a de novo-designed helix-loop-helix polypeptide that homodimerizes and folds into four-helix bundles in the presence of Zn2+, resulting in particle aggregation. Analyte binding interferes with the folding-induced aggregation, giving rise to a clearly detectable colorimetric response.
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| 3. |
- Björk, Per, et al.
(författare)
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Oligothiophene Assemblies Defined by DNA Interaction : From Single Chains to Disordered Clusters
- 2009
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Ingår i: SMALL. - : Wiley. - 1613-6810 .- 1613-6829. ; 5:1, s. 96-103
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Tidskriftsartikel (refereegranskat)abstract
- The organization of conjugated polyelectrolytes (CPEs) interacting with biomolecules sets conditions for the biodetection of biological processes and identity, through the use of optical emission from the CPE. Herein, a well-defined CPE and its binding to DNA is studied. By using dynamic light scattering and circular dichroism spectroscopy, it is shown that the CPE forms a multimolecule ensemble in aqueous solution that is more than doubled it? size when interacting with a small DNA chain, while single chains are evident in ethanol. The related changes in the fluorescence spectra upon polymer aggregation are assigned to oscillator strength redistribution between vibronic transitions in weakly coupled H-aggregates. An enhanced single-molecule spectroscopy technique that allows full control of excitation and emission light polarization is applied to combed and decorated;,DNA chains. It is found that the organization of combed CPE-lambda DNA complexes (when dry on the surface) allows considerable variation of CPE distances and direction relative to the DNA chain. By analysis of the polarization data. energy transfer between the polymer chains in individual complexes is confirmed and their sizes estimated.
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| 4. |
- Björk, Per, et al.
(författare)
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Soft lithographic printing of patterns of stretched DNA and DNA/electronic polymer wires by surface-energy modification and transfer
- 2006
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Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 2:8-9, s. 1068-1074
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Tidskriftsartikel (refereegranskat)abstract
- Aligned and stretched λ DNA is directed to specific locations on solid substrates. Surface-energy modification of glass substrates by using patterned polydimethylsiloxane (PDMS) stamps is used to direct DNA onto the surface-energy-modified micrometer-scale pattern through molecular combing. As an alternative, patterned and nonpatterned PDMS stamps modified with polymethylmethacrylate (PMMA) are utilized to direct the stretched DNA to the desired location and the results are compared. The DNA is elongated through molecular combing on the stamp and transfer printed onto the surfaces. PMMA-modified stamps show a more defined length of the stretched DNA, as compared to bare PDMS stamps. A combination of these two methods is also demonstrated. As an application example, transfer printing of DNA decorated with a semiconducting conjugated polyelectrolyte is shown. The resulting patterned localization of stretched DNA can be utilized for functional nanodevice structures, as well as for biological applications.
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| 5. |
- Bordag, Michael, et al.
(författare)
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Shear stress measurements on InAs nanowires by AFM manipulation
- 2007
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Ingår i: Small. - Weinheim : Wiley-VCH Verlagsgesellschaft. - 1613-6810 .- 1613-6829. ; 3:8, s. 1398-1401
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Tidskriftsartikel (refereegranskat)abstract
- On an upward curve? The curvature of an elastically deformed nanowire pinned to a flat surface contains information about the maximum static friction force, and hence the shear stress, between the nanowire and the surface. Here, InAs nanowires are bent in a controlled manner using the tip of an atomic force microscope (see image). The shear stress can be obtained from a simple analysis according to the standard theory of elasticity.
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| 6. |
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| 7. |
- Cisneros, David A., et al.
(författare)
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Creating ultrathin nanoscopic collagen matrices for biological and biotechnological applications
- 2007
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Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 3:6, s. 956-963
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Tidskriftsartikel (refereegranskat)abstract
- The biofunctionalization of materials creates interfaces on which proteins, cells, or tissues can fulfill native or desired tasks. Here we report how to control the assembly of type I collagen into well-defined nanoscopic matrices of different patterns. Collagen fibrils in these ultrathin (approximately 3 nm) matrices maintained their native structure as observed in vivo. This opens up the possibility to create programmable biofunctionalized matrices using collagen-binding proteins or proteins fused with collagen-binding domains. Applied to eukaryotic cells, these nanostructured matrices can direct cellular processes such as adhesion, orientation and migration.
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| 8. |
- Conache, Gabriela, et al.
(författare)
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Friction measurements of InAs nanowires on Silicon nitride by AFM manipulation
- 2009
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Ingår i: Small. - Weinheim, Germany : Wiley-VCH Verlagsgesellschaft. - 1613-6810 .- 1613-6829. ; 5:2, s. 203-207
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Tidskriftsartikel (refereegranskat)abstract
- A study was conducted to perform friction measurements of InAs nanowires (NW) on silicon nitride (Si 3N 4) through atomic force microscopy (AFM) manipulation. The investigations revealed the friction force per unit length for sliding and static friction over a range of nanowire diameters. It was found that there is a significant difference between the coefficients of the two sliding modes for large wires. It was also found that the difference between the two sliding modes disappears at smaller diameters and the sliding friction becomes equal with the static friction. The AFM investigations were performed on a Nanoscope IIIa Dimension 3100, using rectangular cantilevers, with a nominal spring constant of 30 N m -1. The nanowires were manipulated, using the 'Retrace Lift' mode of the AFM controller. The friction force per unit length was gathered from the local curvature of the NWs, using standard elasticity theory.
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| 9. |
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| 10. |
- Gonzalez-Diaz, J. B., et al.
(författare)
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Plasmonic Au/Co/Au nanosandwiches with enhanced magneto-optical activity
- 2008
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Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 4:2, s. 202-205
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Tidskriftsartikel (refereegranskat)abstract
- A study was conducted to analyzed magneto-optical (MO) activity of thin films prepared by Au/Co/Au trilayers as surface plasmon polaritons. Trilyers exhibit a magnetic-field-induced nonreciprocal effect in the surface plasmon polariton propagation. The Au/Co/Au nanosandwiches, prepared by a self-assembly process, show localized surface plasmon resonances (LSPRs) that affect the optical properties of metallic nanostructures. The fabrication of the nanostructures was performed using colloidal lithography (CL). An atomic force microscopy was used to analyze the structure of thin films. The polar Kerr ellipticity and rotation spectra for an array of nanoparticles with identical dimensions were examined. Metal trilyer films were sputtered onto glass substrates. Results show that nanoparticles can be used for the development of high-sensitive magnetoplasmonic nanosensors with multiplexing capabilities.
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