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Identification of p...
Identification of poly(ADP-ribose) polymerase-1 and Ku70/Ku80 as transcriptional regulators of S100A9 gene expression
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- Grote, Jens (författare)
- Institute of Experimental Dermatology, University of Muenster, Germany; Interdisciplinary Center for Clinical Research, Core Group Integrated Functional Genomics, Medical Faculty, University of Muenster, Muenster, Germany
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- Koenig, Simone (författare)
- Interdisciplinary Center for Clinical Research, Core Group Integrated Functional Genomics, Medical Faculty, University of Muenster, Muenster, Germany
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- Ackermann, Doreen (författare)
- Interdisciplinary Center for Clinical Research, Core Group Integrated Functional Genomics, Medical Faculty, University of Muenster, Muenster, Germany
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- Sopalla, Claudia (författare)
- Institute of Experimental Dermatology, University of Muenster, Germany; Interdisciplinary Center for Clinical Research (IZKF) Münster, Germany
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- Benedyk, Malgorzata (författare)
- Institute of Experimental Dermatology, University of Muenster, Germany
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- Los, Marek Jan (författare)
- Manitoba Institute of Cell Biology, Cancer Care Manitoba; Manitoba Institute of Child Health; Department of Biochemistry and Medical Genetics; Department of Human Anatomy and Cell Science, University Manitoba, Winnipeg, Canada,
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- Kerkhoff, Claus (författare)
- Interdisciplinary Center for Clinical Research (IZKF) Münster, Germany; Institute of Experimental Dermatology, University of Muenster, Germany
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(creator_code:org_t)
- 2006-12-22
- 2006
- Engelska.
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Ingår i: BMC Molecular Biology. - : Springer Science and Business Media LLC. - 1471-2199. ; 7
- Relaterad länk:
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http://www.biomedcen...
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https://liu.diva-por... (primary) (Raw object)
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https://bmcmolbiol.b...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: S100 proteins, a multigenic family of non-ubiquitous cytoplasmic Ca2+-binding proteins, have been linked to human pathologies in recent years. Dysregulated expression of S100 proteins, including S100A9, has been reported in the epidermis as a response to stress and in association with neoplastic disorders. Recently, we characterized a regulatory element within the S100A9 promotor, referred to as MRE that drives the S100A9 gene expression in a cell type-specific, activation- and differentiation-dependent manner (Kerkhoff et al. ( 2002) J. Biol. Chem. 277, 41879-41887). Results: In the present study, we investigated transcription factors that bind to MRE. Using the MRE motif for a pull-down assay, poly(ADP-ribose) polymerase-I (PARP-I) and the heterodimeric complex Ku70/Ku80 were identified by mass spectrometry and confirmed by chromatin immunoprecipitation. Furthermore, TPA-induced S100A9 gene expression in HaCaT keratinocytes was blocked after the pharmacologic inhibition of PARP-l with 1,5- isoquinolinediol (DiQ). Conclusion: The candidates, poly(ADP-ribose) polymerase-l (PARP-l) and the heterodimeric complex Ku70/ Ku80, are known to participate in inflammatory disorders as well as tumorgenesis. The latter may indicate a possible link between S100 and inflammation-associated cancer.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Nyckelord
- activation
- breast-cancer cells
- c/ebp-alpha
- calcium-binding proteins
- dna-damage
- epidermal differentiation
- prostate-cancer
- psoriasis
- skin carcinogenesis
- squamous-cell carcinoma
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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