| 1. |
- Andreeva, HD, et al.
(författare)
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Kinetic of monocytes Fcγ receptors in patients after coronary artery bypass
- 2011
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Ingår i: CENTRAL EUROPEAN JOURNAL OF MEDICINE. - : Walter de Gruyter GmbH. - 1895-1058. ; 6:1, s. 31-36
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Cardiopulmonary bypass is usually associated with an increased risk of post-operative infections and systemic inflammatory response syndrome. This is accompanied by a neutrophil leucocytosis and lymphopenia. Less is known about the role of monocytes and markers of monocyte activity. This study focuses on the changes of Fc-gamma receptors on monocytes in patients undergoing on-pump coronary artery bypass grafting (CABG).The surface expression of CD64, CD32 and CD16 were studied using flow cytometry in 37 patients scheduled for CABG. The antigen density and the percentage positive cells were monitored preoperatively and on day 1, 2, 3 and 7 postoperatively. CD64 and CD32 antigen density were significantly increased from day 1 to day 7(p<0.0001). A significant increase (p<0.0001) in the percentage CD16+ monocytes was detected at day 1,2,and 3. The downregulation of CD16 expression on day 1 was followed be elevation at days 2,3(p<0.01). On day 7th percentage CD16+ monocytes and density were not returned to baseline values. Only the baseline levels of CD64 was lower compared to controls(p<0.05). The results suggest that on-pump CABG induces dynamic changes in the expression of Fc-gamma receptors on monocytes as late as 7 days. We observed significant upregulation in the expression of CD64 and CD32 and “to phases” distribution of CD16 in the post-CABG period.
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| 2. |
- Ghosal, Anubrata, et al.
(författare)
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RNA interference and its therapeutic potential
- 2011
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Ingår i: Central European Journal of Medicine. - : Versita. - 1895-1058 .- 1644-3640. ; 6:2, s. 137-147
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Forskningsöversikt (refereegranskat)abstract
- RNA interference is a technique that has become popular in the past few years. This is a biological method to detect the activity of a specific gene within a cell. RNAi is the introduction of homologous double stranded RNA to specifically target a gene’s product resulting in null or hypomorphic phenotypes. This technique involves the degradation of specific mRNA by using small interfering RNA. Both microRNA (miRNA) and small interfering RNA (siRNA) are directly related to RNA interference. RNAi mechanism is being explored as a new technique for suppressing gene expression. It is an important issue in the treatment of various diseases. This review considers different aspects of RNAi technique including its history of discovery, molecular mechanism, gene expression study, advantages of this technique against previously used techniques, barrier associated with this technique, and its therapeutic application.
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| 3. |
- Jaruseviciene, Lina, et al.
(författare)
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A study of antibiotic prescribing: the experience of Lithuanian and Russian GPs
- 2012
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Ingår i: Central European Journal of Medicine. - : Walter de Gruyter GmbH. - 1644-3640. ; 7:6, s. 790-799
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Tidskriftsartikel (refereegranskat)abstract
- Background. Globally, general practitioners (GPs) write more than 90% of all antibiotic prescriptions. This study examines the experiences of Lithuanian and Russian GPs in antibiotic prescription for upper respiratory tract infections, including their perceptions of when it is not indicated clinically or pharmacologically. Methods. 22 Lithuanian and 29 Russian GPs participated in five focus group discussions. Thematic analysis was used to analyse the data. Results. We identified four main thematic categories: patients' faith in antibiotics as medication for upper respiratory tract infections; patient potential to influence a GP's decision to prescribe antibiotics for upper respiratory tract infections; impediments perceived by GPs in advocating clinically grounded antibiotic prescribing with their patients, and strategies applied in physician-patient negotiation about antibiotic prescribing for upper respiratory tract infections. Conclusions. Understanding the nature of physician-patient interaction is critical to the effective pursuit of clinically grounded antibiotic use as this study undertaken in Lithuania and the Russian Federation has shown. Both physicians and patients must be targeted to ensure correct antibiotic use. Further, GPs should be supported in enhancing their communication skills about antibiotic use with their patients and encouraged to implement a shared decision-making model in their practices.
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| 4. |
- Machaczka, M, et al.
(författare)
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Eosinophilia as a presenting symptom of the metastatic lung adenocarcinoma with an unknown primary localization
- 2011
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Ingår i: CENTRAL EUROPEAN JOURNAL OF MEDICINE. - : Walter de Gruyter GmbH. - 1895-1058. ; 6:5, s. 541-544
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Apparent hematological symptoms rarely dominate the clinical picture of an underlying non-hematological malignancy. Malignancy-associated eosinophilia can result from clonal or non-clonal proliferation of eosinophils. Here, we report the case of a 59-year-old man with metastatic adenocarcinoma of the lung with an unknown primary tumor site, which presented as hypereosinophilia, anemia, lymphadenopathy, weight loss, and malaise. Bone marrow biopsy disclosed metastatic adenocarcinoma positive in immunohistochemistry for cytokeratin 7. Further assessment of specimens obtained from the bronchoalveolar lavage and biopsy of the mediastinal lymph nodes confirmed the diagnosis of the metastatic lung cancer, although the primary tumor site remained undiscovered. This case underlines that eosinophilia may represent a rare primary manifestation of an undetected malignancy, and it is thus important to consider this as part of the differential diagnosis in patients presenting with unexplained eosinophilia.
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| 5. |
- Stankunas, M, et al.
(författare)
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Differences in reporting somatic complaints in elderly by education level
- 2013
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Ingår i: Central European Journal of Medicine. - : Walter de Gruyter GmbH. - 1895-1058 .- 1644-3640. ; 8:1, s. 125-131
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Tidskriftsartikel (refereegranskat)abstract
- Aim: To evaluate the association between somatic complaints and education level among elders in selected European countries. Methods: Cross-sectional study among randomly selected community dwelling persons aged 60–84 years from seven cities in Europe: Ancona (Italy), Athens (Greece), Granada (Spain), Kaunas (Lithuania), Stuttgart (Germany), Porto (Portugal) and Stockholm (Sweden). Somatic complaints were measured with the 24-item version of the Giessen Complaint List (GBB-24). A regression analysis was done to investigate the association between education and somatic complaints. Results. The mean GBB-24 scale was 16.3±14.9. The most common complaints were pain in joints and limbs (29.6%), back-pain (24.1%), heaviness or tiredness in the legs (19.1%) and general tiredness (15.7%). Respondents with less than primary education reported higher levels of somatic symptoms compared to those with university degree. A regression analysis showed that higher education (OR=0.69) and being male (OR=0.48) were associated with a decreased risk for high levels of somatic complaints, and the opposite for older age (OR=1.03) and being single/divorced/window (OR=1.28). Conclusions: The results indicate that less educated elders are more likely to expressed somatic complaints.
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| 6. |
- Stankuniene, Aurima, et al.
(författare)
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Use of medications amongst older persons in Kaunas, Lithuania
- 2013
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Ingår i: Open Medicine. - : Walter de Gruyter GmbH. - 1911-2092 .- 2391-5463. ; 8:6, s. 855-860
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Tidskriftsartikel (refereegranskat)abstract
- Aim: To evaluate the associations between socio-economical factors and the use of medications in the elderly. Methods: The data was collected in a cross-sectional study in 2009. We received 624 completed questionnaires (response rate - 48.9%) from elderly people aged 60-84 years living in Kaunas (Lithuania). For an evaluation of the impact of explanatory variables on the analyzed events (binary dependent variable), an Enter model of logistic regression was used. Results: Our findings suggest that 50.8% (n=317) of respondents used at least one drug daily. 18.3% (n=114) of respondents indicated that they use medications regularly, but not on a day-by-day basis. One quarter (25.6%; n=160) used medication only on an "at need" basis. Only 5.3% (n=33) of older persons did not use any medications at all. Logistic regression showed that being male (OR=0.67; 95%CI:0.45-0.98) was associated with using medications "regularly + daily." For the use of "daily" medications, older age (OR=1.33; 95%CI:1.15-1.53) was associated with using medications daily. An opposite association was observed for respondents having no paid work (OR=0.48; 95%CI:0.26-0.82). Conclusions: Our study suggests that more than half of older persons in Lithuania use medications every day. Use was associated with socioeconomic factors (gender, age, and employment status).
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