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- Eliasson, Charlotte, 1973, et al.
(författare)
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Multivariate evaluation of doxorubicin surface-enhanced Raman spectra.
- 2001
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Ingår i: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. - 1386-1425. ; 57:9, s. 1907-15
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Tidskriftsartikel (refereegranskat)abstract
- Multivariate evaluation of surface-enhanced Raman spectra of doxorubicin in plasma was performed. In a principal component analysis (PCA) all spectral features were modelled into three principal components. The major variation of the data was shown to be the variation of doxorubicin Raman signal together with the doxorubicin fluorescence, whereas the variation due to plasma was of minor importance. It was also shown that the surface-enhanced Raman scattering (SERS) measurements were independent on such factors as measurement occasion and silver colloids. The presented results show that with some improvements, quantification of doxorubicin directly in plasma could be possible.
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| 2. |
- Lorén, Anders, 1974, et al.
(författare)
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Feasibility of quantitative determination of doxorubicin with surface-enhanced Raman spectroscopy
- 2001
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Ingår i: Journal of Raman Spectroscopy. - : Wiley. - 0377-0486 .- 1097-4555. ; 32:11, s. 971-974
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Tidskriftsartikel (refereegranskat)abstract
- Surface-enhanced Raman spectroscopy (SERS) was performed using excitation at 488 nm in a blood plasma-doxorubicin-silver colloid system. With a blood plasma content of 1%, a partial least-squares calibration of the doxorubicin was made in the 10-750 nM range. Predictions for a test set generated a root mean square error of prediction of 70 nM. The use of SERS and chemometrics in complex systems made it possible to use the highly informative Raman signals even at low concentrations without the need for sample pretreatment such as extraction. Copyright © 2001 John Wiley & Sons, Ltd.
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| 3. |
- Lorén, Anders, 1974, et al.
(författare)
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Internal standard in surface-enhanced Raman spectroscopy
- 2004
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 76:24, s. 7391-7395
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Tidskriftsartikel (refereegranskat)abstract
- A method is presented for the use of SAM layers as internal standards for calibration in surface-enhanced Raman spectroscopy. Three cyano-containing compounds were attached to gold colloids via a metal-sulfur bond and evaluated for spectral stability and normalization capacity. The results show that the analyte, rhodamine 6G, and the internal standard signal enhancement covaried, and it was possible to quantify the analyte with PLS. The fact that the enhancing substrate was chaotic assemblies with large variation in signal enhancement shows the versatility of this method.
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| 4. |
- Lorén, Anders, 1974, et al.
(författare)
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Self-assembled monolayer coating for normalization of surface enhanced Raman spectra
- 2004
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Ingår i: Nano Letters. - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 4:2, s. 309-312
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate that the use of a self-assembled monolayer, consisting of a thiol derivative of Dabcyl, can be used to normalize surface enhanced Raman signals (SERS) with respect to varying enhancement. Chaotic assemblies of gold nanoparticles exhibit large spatial variation in enhancement. Our work shows that in such a system the signals from the reporting molecules in the SAM co-vary with the signal from the analyte solution. With this knowledge, a normalization procedure was used to increase the precision of the analyte signal by 1 order of magnitude, to 8-13%, fully acceptable for quantitative work.
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| 7. |
- Ek, Torben, 1963, et al.
(författare)
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Intensive treatment for childhood acute lymphoblastic leukemia reduces immune responses to diphtheria, tetanus, and Haemophilus influenzae type b
- 2004
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Ingår i: J Pediatr Hematol Oncol. - 1077-4114. ; 26:11, s. 727-34
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVES: Immunity to diphtheria toxoid (D), tetanus toxoid (T), and Haemophilus influenzae type b (Hib) is affected in children with acute lymphoblastic leukemia (ALL). The aims were to examine immunity and to compare the response to immunization at 1 or 6 months after treatment. METHODS: Thirty-one patients were immunized with DT and conjugated Hib vaccine (ActHib) at 1 month or 6 months after treatment of ALL with the NOPHO 92 protocol. Antibody levels were determined before and 3 weeks after vaccination. Specific T and Hib antibody-secreting cells of IgG/IgA/IgM isotypes were analyzed in peripheral blood using an ELISPOT technique. RESULTS: All specific antibody levels decreased during ALL treatment, and protective levels after treatment were noted for 17% against D, 33% against T, and 100% against Hib. No high-risk patient had full D or T protection after treatment. After vaccination all the standard- and intermediate-risk patients achieved full protection against D, T, and Hib. The high-risk group showed insufficient immune response (full protection after vaccination: D 56%, T 22%, Hib 78%). No difference was found between vaccination at 1 month or 6 months after treatment. The poor antibody production in the high-risk group correlated to low numbers of antibody-secreting cells. CONCLUSIONS: Nonprotective antibody levels against D, T, and Hib after childhood ALL are more common than previously thought. Insufficient immune response was restricted to the high-risk group and was related to a low number of memory B cells in this study. Immunizations should be included in follow-up after childhood ALL, and the policy should be adapted to treatment intensity.
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| 8. |
- Ek, Torben, 1963, et al.
(författare)
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Proinflammatory cytokines mediate the systemic inflammatory response associated with high-dose cytarabine treatment in children.
- 2001
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Ingår i: Medical and pediatric oncology. - 0098-1532. ; 37:5, s. 459-64
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Tidskriftsartikel (refereegranskat)abstract
- Treatment with high-dose cytarabine (1-beta-D-arabinofuranosylcytosine) is often associated with an acute febrile reaction sometimes including abdominal pain, myalgia, and rash. The similarity of these symptoms to those caused by hypersecretion of cytokines in the systemic inflammatory response syndrome (SIRS) prompted us to investigate the plasma levels of proinflammatory cytokines during treatment of children with high-dose cytarabine.
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