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Search: WFRF:(Alonso Alfonso) > (2020)

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  • Alonso Vicario, S., et al. (author)
  • Unravelling the influence of human behaviour on reducing casualties during flood evacuation
  • 2020
  • In: Hydrological Sciences Journal. - : Informa UK Limited. - 0262-6667 .- 2150-3435. ; 65:14, s. 2359-2375
  • Journal article (peer-reviewed)abstract
    • Floods are the natural hazards that are causing the most deaths worldwide. Flood early warning systems are one of the most cost-efficient methods to reduce death rates, triggering decisions about the evacuation of exposed population. Although previous studies have investigated the effect of human behaviours on evacuation processes, studies analysing a combination of behaviours, flood onset and warning timing are limited. Our objective is to explore how changes on the aforementioned factors can affect casualties. This is done within a modelling framework that includes an agent-based model, a hydraulic model, and a traffic model, which is implemented for the case study of Orvieto (Italy). The results show that the number of casualties is most impacted by people's behaviour. Besides, we found that a delay of 30 min in releasing the warning can boost the number of casualties up to six times. These results may help managers to propose effective emergency plans.
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2.
  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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