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- James, A., et al.
(författare)
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Serum periostin relates to type-2 inflammation and lung function in asthma : Data from the large population-based cohort Swedish GA(2)LEN
- 2017
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Ingår i: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538 .- 1398-9995. ; 72:11, s. 1753-1760
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundPeriostin has been suggested as a novel, phenotype-specific biomarker for asthma driven by type 2 inflammation. However, large studies examining relationships between circulating periostin and patient characteristics are lacking and the suitability of periostin as a biomarker in asthma remains unclear.AimTo examine circulating periostin in healthy controls and subjects with asthma from the general population with different severity and treatment profiles, both with and without chronic rhinosinusitis (CRS), in relation to other biomarkers and clinical characteristics.MethodsSerum periostin was examined by ELISA in 1100 subjects aged 17-76 from the Swedish Global Allergy and Asthma European Network (GA(2)LEN) study, which included 463 asthmatics with/without chronic rhinosinusitis (CRS), 98 individuals with CRS only, and 206 healthy controls. Clinical tests included measurement of lung function, Fraction of exhaled NO (FeNO), IgE, urinary eosinophil-derived neurotoxin (U-EDN), and serum eosinophil cationic protein (S-ECP), as well as completion of questionnaires regarding respiratory symptoms, medication, and quality of life.ResultsAlthough median periostin values showed no differences when comparing disease groups with healthy controls, multiple regression analyses revealed that periostin was positively associated with higher FeNO, U-EDN, and total IgE. In patients with asthma, an inverse relationship with lung function was also observed. Current smoking was associated with decreased periostin levels, whereas increased age and lower body mass index (BMI) related to higher periostin levels in subjects both with and without asthma.ConclusionWe confirm associations between periostin and markers of type 2 inflammation, as well as lung function, and identify novel constitutional factors of importance to the use of periostin as a phenotype-specific biomarker in asthma.
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- Patelis, Antonios, et al.
(författare)
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IgE sensitization to food allergens and airborne allergens in relation to biomarkers of type 2 inflammation in asthma
- 2018
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Ingår i: Clinical and Experimental Allergy. - : John Wiley & Sons. - 0954-7894 .- 1365-2222. ; 48:9, s. 1147-1154
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: We have recently reported that sensitisation to food allergens and sensitisation to airborne allergens had independent associations with increased fraction of exhaled nitric oxide (FeNO) and blood eosinophils in middle-aged adults and in young subjects with asthma.OBJECTIVE: To investigate the relation between IgE sensitisation and several type 2 inflammation biomarkers in adult asthmatics.METHODS: FeNO, urinary eosinophil-derived neurotoxin (U-EDN), serum eosinophil cationic protein (S-ECP) and periostin were measured in 396 asthmatics, aged 17-76 years, from the Swedish GA2LEN study. Sensitisation to airborne allergens was examined with skin prick tests (≥3 mm wheal) and sensitisation to food allergens with measurement of specific IgE (≥0.35kU/L).RESULTS: Asthmatics sensitised to food allergens had higher FeNO, 22.3 ppb (18.6, 26.7) vs. 16.1 ppb (14.2, 18.2) (p=0.005), S-ECP, 17.7 mg/L (14.8, 21.1) vs. 12.8 mg/L (10.9, 14.9) (p=0.01), and periostin, 73.7 (67.5, 80.3) ng/mL vs. 59.9 (55.8, 64.2) ng/mL (p=0.003), than non-sensitised subjects. Periostin levels in this group were also significantly higher than in the group sensitised only to airborne allergens (p=0.01). Sensitisation to food allergens related independently to FeNO (p=0.02), S-ECP (p=0.006) and periostin (p=0.004), whereas sensitisation only to airborne allergens related only to FeNO (p=0.02) after adjustments for age, sex, height, weight and smoking history. FeNO correlated weakly with S-ECP (r=0.17, p<0.001), periostin (r=0.19, p<0.001) and U-EDN (0.16, p<0.001). S-ECP also correlated weakly with U-EDN (r=0.12, p=0.02). None of the correlations between the remaining pairs of markers of type 2 inflammation were significant.CONCLUSIONS & CLINICAL RELEVANCE: Sensitisation to food allergens related to several local and systemic type 2 inflammation markers, such as FeNO, S-ECP and periostin. Assessing the profile of allergic sensitisation, including to food allergens, might improve the understanding and interpretation of inflammatory markers and potentially improve asthma management.
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- Öhrmalm, L., et al.
(författare)
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Presence of rhinovirus in the respiratory tract of adolescents and young adults with asthma without symptoms of infection
- 2016
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Ingår i: Respiratory Medicine. - : Elsevier BV. - 0954-6111 .- 1532-3064. ; 115, s. 1-6
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Tidskriftsartikel (refereegranskat)abstract
- Background: Viral respiratory infections have been associated with up to 80% of wheezing episodes and asthma exacerbations. However, studies on the role of these viruses in asthmatic patients in the interval between exacerbations are sparse. This study aimed to determine the presence of respiratory viruses, without symptoms of infection, in the airways of young asthmatics as compared to healthy controls.Material and Methods: Patients 10-35 years of age with stable asthma and a group of healthy controls were analyzed regarding the presence of RNA from common respiratory viruses in nasopharyngeal aspirates by PCR. Self-reported asthma control and quality of life, fraction of exhaled nitric oxide (FeNO), spirometry, and bronchial responsiveness to methacholine were recorded. Blood samples were collected to assess IgE sensitisation and eosinophil cationic protein (ECP) levels.Results: In 354 patients with asthma and 108 healthy controls, human rhinovirus (HRV) was the only virus detected (4.5% of asthmatics vs. 0.9% of controls; p = 0.08). HRV+ asthma patients had a higher degree of aeroallergen IgE sensitisation (median 37.7 vs. 10.4 kU(A)/L, p = 0.04), and a tendency for higher levels of serum ECP (median 17.2 vs. 12.6 mu g/L, p = 0.07), as compared to their HRV- counterparts.Conclusions: Absence of symptoms of respiratory tract infection notwithstanding, HRV seems to be more prevalent in the airways of adolescents and young adults with asthma and a high degree of aeroallergen IgE sensitisation than in controls. The presence of HRV seems also to be related to systemic eosinophilic inflammation despite ongoing treatment with inhaled corticosteroids.
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