| 1. |
- Anderson, Emma, 1975-, et al.
(författare)
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Oligodendrocytens nyckelroll
- 2000
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Ingår i: Läkartidningen. - 0023-7205 .- 1652-7518. ; 97, s. 3265-3268
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 2. |
- Anderson, Emma S., 1975-, et al.
(författare)
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Developing chicken oligodendrocytes express the type IV oligodendrocyte marker T4-O in situ, but not in vitro
- 2000
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Ingår i: Neuroscience Letters. - 0304-3940 .- 1872-7972. ; 284:1-2, s. 21-24
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Tidskriftsartikel (refereegranskat)abstract
- Accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. We recently reported that a polyclonal antiserum against an unknown antigen, the T4-O molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type IV variety of Del Rio Hortega. The present study examines the developmental expression of the T4-O molecule in situ and in vitro. The results show that T4-O immunoreactive cells first appear at E15 in the ventral funiculus. But, oligodendrocytes cultured in vitro with or without neurones do not develop a T4-O immunoreactivity. We conclude that oligodendrocytes in the spinal cord of chicken embryos first express the T4-O molecule some time after onset of myelination, and that the T4-O immunoreactive phenotype does not develop in vitro.
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| 3. |
- Anderson, Emma S., et al.
(författare)
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Myelination of prospective large fibres in chicken ventral funiculus
- 2000
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Ingår i: Journal of Neurocytology. - 0300-4864 .- 1573-7381. ; 29:10, s. 755-764
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Tidskriftsartikel (refereegranskat)abstract
- In mammals, the oligodendrocyte population includes morphological and molecular varieties. We reported previously that an antiserum against the T4-O molecule labels a subgroup of oligodendrocytes related to large myelinated axons in adult chicken white matter. We also reported that T4-O immunoreactive cells first appear in the developing ventral funiculus (VF) at embryonic day (E)15, subsequently increasing rapidly in number. Relevant fine structural data for comparison are not available in the literature. This prompted the present morphological analysis of developing and mature VF white matter in the chicken. The first axon-oligodendrocyte connections were seen at E10 and formation of compact myelin had started at E12. Between E12 and E15 the first myelinating oligodendrocytes attained a Schwann cell-like morphology. At hatching (E21) 60% of all VF axons were myelinated and in the adult this proportion had increased to 85%. The semilunar or polygonal oligodendrocytes associated with adult myelinated axons contained many organelles indicating a vivid metabolic activity. Domeshaped outbulgings with gap junction-like connections to astrocytic profiles were frequent. Oligodendrocytes surrounded by large myelinated axons and those surrounded by small myelinated axons were cytologically similar. But, thick and thin myelin sheaths had dissimilar periodicities and Marchi-positive myelinoid bodies occurred preferentially in relation to large myelinated axons. We conclude that early oligodendrocytes contact axons and form myelin well before the first expression of T4-O and that emergence of a T4-O immunoreactivity coincides in time with development of a Type IV phenotype. Our data also show that oligodendrocytes associated with thick axons are cytologically similar to cells related to thin axons. In addition, the development of chicken VF white matter was found to be similar to the development of mammalian white matter, except for the rapid time course.
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