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- Andersson, Rolf E.
(författare)
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Concentration and partial purification of lipase from Pseudomonas fluorescens
- 1980
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Ingår i: Biotechnology letters. - 0141-5492 .- 1573-6776. ; 2:5, s. 247-252
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Tidskriftsartikel (refereegranskat)abstract
- During ultrafiltration in a hollow fiber device 105 liters of lipase frort Pseudomonas fluorescens was concentrated to 4 liters with a yield of 56% of total initial activity. The concentrated lipase solution was lyophilized and purified on a DEAE-cellulose anion exchanger column. The partly purified lipase was found to probably contain carbohydrates. © 1980 Kluwer Academic Publishers.
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3. |
- Andersson, Rolf E., et al.
(författare)
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HPLC analysis of organic acids in lactic acid fermented vegetables
- 1983
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Ingår i: Zeitschrift für Lebensmittel-Untersuchung und -Forschung. - 0044-3026. ; 176:6, s. 440-443
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Tidskriftsartikel (refereegranskat)abstract
- An HPLC technique is described for the analysis of organic acids in lactic acid fermented vegetables. An Aminex HPX-87 column with 0.013 M-H 2SO 4 as mobile phase was used. Separation data are presented for oxalic, citric, tartaric, malic, succinic, lactic, formic, acetic, propionic, and butyric acids. Interference problems from sugars are also reported. © 1983 J. F. Bergmann Verlag.
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5. |
- Andersson, Rolf E.
(författare)
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Microbial lipolysis at low temperatures
- 1980
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Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 39:1, s. 36-40
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Tidskriftsartikel (refereegranskat)
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7. |
- Toll, Johan B, et al.
(författare)
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Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB
- 1981
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Ingår i: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538 .- 1398-9995. ; 36:6, s. 411-417
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Tidskriftsartikel (refereegranskat)abstract
- This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50--95% and the recovery was 30--40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests.
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