| 1. |
- Iglesias-Gato, Diego, et al.
(författare)
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The Proteome of Primary Prostate Cancer
- 2016
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Ingår i: European Urology. - : Elsevier. - 0302-2838 .- 1873-7560. ; 69:5, s. 942-952
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Tidskriftsartikel (refereegranskat)abstract
- Background: Clinical management of the prostate needs improved prognostic tests and treatment strategies. Because proteins are the ultimate effectors of most cellular reactions, are targets for drug actions and constitute potential biomarkers; a quantitative systemic overview of the proteome changes occurring during prostate cancer (PCa) initiation and progression can result in clinically relevant discoveries.Objectives: To study cellular processes altered in PCa using system-wide quantitative analysis of changes in protein expression in clinical samples and to identify prognostic biomarkers for disease aggressiveness.Design, setting, and participants: Mass spectrometry was used for genome-scale quantitative proteomic profiling of 28 prostate tumors (Gleason score 6-9) and neighboring nonmalignant tissue in eight cases, obtained from formalin-fixed paraffin-embedded prostatectomy samples. Two independent cohorts of PCa patients (summing 752 cases) managed by expectancy were used for immunohistochemical evaluation of proneuropeptide-Y (pro-NPY) as a prognostic biomarker.Results and limitations: Over 9000 proteins were identified as expressed in the human prostate. Tumor tissue exhibited elevated expression of proteins involved in multiple anabolic processes including fatty acid and protein synthesis, ribosomal biogenesis and protein secretion but no overt evidence of increased proliferation was observed. Tumors also showed increased levels of mitochondrial proteins, which was associated with elevated oxidative phosphorylation capacity measured in situ. Molecular analysis indicated that some of the proteins overexpressed in tumors, such as carnitine palmitoyltransferase 2 (CPT2, fatty acid transporter), coatomer protein complex, subunit alpha (COPA, vesicle secretion), and mitogen-and stress-activated protein kinase 1 and 2 (MSK1/2, protein kinase) regulate the proliferation of PCa cells. Additionally, pro-NPY was found overexpressed in PCa (5-fold, p < 0.05), but largely absent in other solid tumor types. Pro-NPY expression, alone or in combination with the ERG status of the tumor, was associated with an increased risk of PCa specific mortality, especially in patients with Gleason score <= 7 tumors.Conclusions: This study represents the first system-wide quantitative analysis of proteome changes associated to localized prostate cancer and as such constitutes a valuable resource for understanding the complex metabolic changes occurring in this disease. We also demonstrated that pro-NPY, a protein that showed differential expression between high and low risk tumors in our proteomic analysis, is also a PCa specific prognostic biomarker associated with increased risk for disease specific death in patients carrying low risk tumors.Patient summary: The identification of proteins whose expression change in prostate cancer provides novel mechanistic information related to the disease etiology. We hope that future studies will prove the value of this proteome dataset for development of novel therapies and biomarkers. (C) 2015 European Association of Urology. Published by Elsevier B.V. All rights reserved.
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| 2. |
- Gräslund, Anne-Sofie, 1940-, et al.
(författare)
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Akademien granskar... : Arkeologi
- 2015
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Ingår i: Saga och Sed.. Kungl. Gustav Adolfs akademiens årsbok. - Uppsala. - 0586-5360. ; 2014, s. 137-154
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Ali, Imran, et al.
(författare)
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Exposure to polychlorinated biphenyls and prostate cancer : population-based prospective cohort and experimental studies
- 2016
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Ingår i: Carcinogenesis. - : Oxford University Press. - 0143-3334 .- 1460-2180. ; 37:12, s. 1144-1151
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Tidskriftsartikel (refereegranskat)abstract
- Polychlorinated biphenyls (PCBs) are highly persistent environmental pollutants and are undesirable components of our daily food. PCBs are classified as human carcinogens, but the evidence for prostate cancer is limited and available data are inconsistent. We explored the link between non-dioxin-like PCB and grade of prostate cancer in a prospective cohort as well as in cell experiments. A population-based cohort of 32496 Swedish men aged 45-79 years was followed prospectively through 1998-2011, to assess the association between validated estimates of dietary PCB exposure and incidence of prostate cancer by grade (2789 cases, whereof 1276 low grade, 756 intermediate grade, 450 high grade) and prostate cancer mortality (357 fatal cases). In addition, we investigated a non-dioxin-like PCB153-induced cell invasion and related markers in normal prostate stem cells (WPE-stem) and in three different prostate cancer cell lines (PC3, DU145 and 22RV1) at exposure levels relevant to humans. After multivariable-adjustment, dietary PCB exposure was positively associated with high-grade prostate cancer, relative risk (RR) 1.35 [95% confidence interval (CI): 1.03-1.76] and with fatal prostate cancer, RR 1.43 (95% CI: 1.05-1.95), comparing the highest tertile with the lowest. We observed no association with low or intermediate grade of prostate cancer. Cell invasion and related markers, including MMP9, MMP2, Slug and Snail, were significantly increased in human prostate cancer cells as well as in prostate stem cells after exposure to PCB153. Our findings both from the observational and experimental studies suggest a role of non-dioxin-like PCB153 in the development of high-grade and fatal prostate cancer.
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| 4. |
- Andrén, Anders, 1952-
(författare)
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Archaeology
- 2018
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Ingår i: Handbook of pre-modern Nordic memory studies. - Berlin : Walter de Gruyter. - 9783110440201 ; , s. 135-150
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Bokkapitel (refereegranskat)abstract
- Memory in archaeology is above all about cultural memory, or about how the past was constructed and apprehended in the past. This role of the past in the past has attracted a growing interest in archaeological research in recent decades. Memory studies can be found in archaeology in general, as well as in Scandinavian archaeology. Memory or the role of the past in the past, however, is not possible to understand without reference to time, which means that the construction of time in archaeology is crucial for any discussion of memory (cf. Andrén 2013a, 2015).
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| 5. |
- Andrén, Anders, 1952-
(författare)
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Arkeologi
- 2015
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Ingår i: Forskningens framtid!. - Stockholm : Vetenskapsrådet. - 9789173072526 ; , s. bil. 1-5
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- En kritisk översikt över svensk arkeologi under de senaste 30 åren
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| 6. |
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| 7. |
- Andrén, Anders, 1952-
(författare)
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En fråga om tid
- 2015
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Ingår i: Kungl. Vitterhets historie och antikvitets akademiens årsbok. - Stockholm : Kungl. Vitterhets Historie och Antikvitets Akademien. - 9789174024418 ; , s. 177-186
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 8. |
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| 9. |
- Andrén, Anders, 1952-
(författare)
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Jüdische Friedhöfe im mitteralterlichen Reich, by Susanne Härtel
- 2019
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Ingår i: English Historical Review. - : Oxford University Press (OUP). - 0013-8266 .- 1477-4534. ; 134:570, s. 1270-1271
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Recension (övrigt vetenskapligt/konstnärligt)abstract
- This book by Susanne Härtel is not a comprehensive survey of all 150 medieval Jewish graveyards in the Holy Roman Empire, although the title may create such expectations in the reader. Instead, it is an in-depth study of the complex relationships between the Jewish minorities and the Christian majorities in five German-speaking cities—Magdeburg, Dortmund, Speyer, Regensburg and Zürich. The investigation is a methodological ‘experiment’, based on five different aspects of the graveyards in these cities, namely their location, spatial demarcation, the dead themselves, the gravestones and the visitors. These aspects are in turn analysed from three phenomenological perspectives: visuality, practice and semantics. Through this grid of fifteen viewpoints, Härtel discusses religious differences and similarities between Jews and Christians, and to what extent the religious categories were more important than other distinctions in medieval urban culture, such as gender, professions and social status. By using cemeteries as a starting point, the author aims to study the everyday encounters between Jews and Christians, instead of the more well-known outbreaks of prosecution and expulsion of Jews from medieval cities.
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| 10. |
- Andrén, Anders, 1952-
(författare)
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Landscape and settlement as utopian space
- 2016
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Ingår i: Medieval Archaeology. - : Routledge. - 9780415718165 ; , s. 236-253
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Bokkapitel (refereegranskat)abstract
- An interpretation of the mental and ideological perspectives of landscape and settlement in medieval Svandinavia.
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