| 1. |
- Hafez Ghoran, Salar, et al.
(författare)
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Dammarane-type triterpenoid saponins from Salvia russellii Benth
- 2021
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 184
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Tidskriftsartikel (refereegranskat)abstract
- Three undescribed dammarane-type saponins, russelliinosides A-C, together with a common sterol (β-sitosterol), an abietane diterpenoid (18-hydroxyferruginol), two oleane triterpenoids (daturaolone and oleanolic acid), an ursane triterpenoid (ursolic acid) as well as three 5-hydroxyflavones (cirsimaritin, eupatorin, and salvigenin) were isolated from a dichloromethane extract of the aerial parts of Salvia russellii Benth. The chemical structures of the aforementioned compounds were characterized, using detailed spectroscopic analyses, including high-resolution mass spectrometry and 1D and 2D NMR (1H–1H COSY, TOCSY, HSQC, HMBC and NOESY) spectroscopy as well as physicochemical properties. Cytotoxic effects of S. russellii extract and the three isolated russelliinosides were tested against MCF-7 human breast and A549 lung cancer, as well as non-cancer NIH/3T3 cells using MTT reduction assay. Russelliinosides A and B exhibited cytotoxic activities with IC50 values of 7.1 and 30.7 μg/ml against MCF-7 and 33.9 and 69.4 μg/ml against A549 cells, respectively, while russelliinoside C did not show cytotoxicity against cancer cells. On the other hand, russelliinoside A showed an IC50 value of 31.5 μg/ml against NIH/3T3 cells, while russelliinosides B and C had no effect on the viability of these non-cancer cells.
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| 2. |
- Jassbi, Amir Reza, et al.
(författare)
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Cytotoxic abietane-type diterpenoids from roots of Salvia spinosa and their in Silico pharmacophore modeling
- 2022
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Ingår i: Natural Product Research. - : Informa UK Limited. - 1478-6419 .- 1478-6427. ; 36:12, s. 3183-3188
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Tidskriftsartikel (refereegranskat)abstract
- The roots of Salvia spinosa L. (Lamiaceae) were extracted withhexane, dichloromethane (DCM) and ethyl acetate. The DCMextract exhibited cytotoxic activity (IC50 32.7 mg/mL) against MFC7 breast cancer cell line in MTT colorimetric bioassay. Ferruginol(1), taxodione (2), 12-deoxy-6-hydroxy-6,7-dehydroroyleanone (3),14-deoxycoleon U (4), 15-deoxyfuerstione (5) and taxodone (6)were isolated from the DCM roots extract. Their structures wereelucidated by a combination of spectroscopic analyses includingEIMS and 1H- and 13C NMR spectra. The cytotoxicity of compound3 was determined against MCF-7 and K562 cell lines and compared with the other compounds. A pharmacophore model wasbuilt based on potent input compounds to resolve importantpharmacophore features responsible for cytotoxic activity of theisolated compounds
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| 3. |
- Samadzadeh, Samira, et al.
(författare)
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Characterization and antimicrobial activity of fungal endophytes from Crocus caspius (Iridaceae)
- 2022
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Ingår i: Biocatalysis and Agricultural Biotechnology. - : Elsevier BV. - 1878-8181. ; 43
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Tidskriftsartikel (refereegranskat)abstract
- Plant endophytes are known as one of the newest natural sources for producing specialized metabolites and novel antibiotics. In the present study, endophytic fungi isolated from the corms of Crocus caspius Fisch. & C.A.Mey. ex Hohen. (Iridaceae) were characterized for the first time. Six fungal endophytes were isolated and their antibacterial activity was studied against human pathogens including Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Enterococcus faecium (Clinical isolate), and Candida albicans ATCC 10231 by determination of the minimum inhibitory-minimum bactericidal concentration (MIC-MBC) assays. Thin-layer chromatography (TLC)-bioautography technique was applied to investigate the antifungal activity of the methanolic extracts of fungal endophytes against ochratoxin A-producing fungus, Aspergillus ochraceus Strain 168. Based on morphological and ITS1-5.8S-ITS2 sequencing analysis, isolated endophytes from the plant corms were identified as Xenoacremonium falcatus (SBU-1), Fusarium oxysporum (SBU-2), Cadophora luteo-olivacea (SBU-3), Phialocephala sp. (SBU-4) and Talaromyces purpureogenus (SBU-5 and SBU-6). The extracts of T. purpureogenus SBU-6 (MIC = 1 mg/ml) and Phialocephala sp. SBU-4 (MIC = 0.25 mg/ml) showed a considerable antibacterial activity against E. coli and S. aureus, respectively. The obtained extract from SBU-5 showed the highest antifungal activity (MIC = 4 mg/ml) against C. albicans. Furthermore, both SBU-2 and SBU-5 at a concentration of 16 mg/ml showed bactericidal and fungicidal effects against E. coli and C. albicans. The extracts of SBU-2, SBU-4, and SBU-5 showed significant antifungal activity against A. ochraceous. These findings could be interesting for further exploitation of these endophytes to produce antibiotics and biological fungicides.
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| 4. |
- Zare, Somayeh, et al.
(författare)
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Antidiabetic and cytotoxic polyhydroxylated oleanane and ursane type triterpenoids from Salvia grossheimii
- 2020
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Ingår i: Bioorganic chemistry. - : Elsevier BV. - 0045-2068. ; 104
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Tidskriftsartikel (refereegranskat)abstract
- Two polyhydroxylated oleanane and seven ursane triterpenoids were isolated from aerial parts of Salvia grossheimii. The chemical structures of the undescribed triterpenoids (1–6) were characterized using 1 and 2 D NMR and ESI-MS spectral data as; 2α, 3β, 11α –trihydroxy-olean-12- ene (1), 2α, 3β, 11α-trihydroxy-olean-18-ene (2), 2α- acetoxy-urs-12-ene-3β, 11α, 20β-triol (3), 3-keto-urs-12-ene-1β, 11α, 20β -triol (4), 2α, 3β-diacetoxy-urs-12-ene-1β, 11α, 20β -triol (5), and 3β-acetoxy-urs-12-ene-1β, 11α, 20β –triol (6). All compounds were evaluated for the in vitro α-glucosidase inhibitory and cytotoxic activities against MCF-7 human cancer cell line. Compounds 1, 2, 4, and 6 showed in vitro α-glucosidase inhibitory activity with IC50 = 43.6–198.4 µM, which were more potent than the antidiabetic medicine, acarbose. The remaining compounds; 3, and 7–9 showed potent cytotoxic activity (IC50 = 6.2–31.9 µM) against the cancerous cell line, while the potent α-glucosidase inhibitors were inactive. Molecular docking analysis and kinetic studies were applied to investigate the structure activity relationships and mechanisms of the human and Saccharomyces cerevisiae α-glucosidase inhibitory of the purified compounds. Comparing the high cytotoxicity and α-glucosidase inhibitory of the oleanane and ursane type triterpenoids suggest them as potential lead compounds for further research in anticancer and antidiabetic research.
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