| 1. |
- Mehmood, Muhammad Asim, et al.
(författare)
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CFD study of pressure loss characteristics of multi-holed orifice plates using central composite design
- 2019
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Ingår i: Flow Measurement and Instrumentation. - : Elsevier. - 0955-5986 .- 1873-6998. ; 70, s. 1-11
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Tidskriftsartikel (refereegranskat)abstract
- The study on the use of multi-holed orifice plate for measuring flow rate is a growing area of research. As compared to standard orifice plate, multi-holed orifice plates (MO) have number of advantages, such as, these plates require minimum straight piping at the upstream without compromising the pressure losses and provide better accuracy in the measurement of flow rates. In this study, a systematic methodology is adopted for investigating the effect of different geometrical parameters on pressure loss coefficient and values of parameters under investigation varied using central composite design. The geometrical parameters chosen for the study are: (a) Number of holes; (b) Multi-hole Diameter ratio and (c) Compactness of holes. Commercial computational fluid dynamics code (ANSYS Fluent) is employed to perform simulations for 15 different settings of these parameters to analyze their effect on pressure loss coefficient and flow development length at downstream of multi-holed orifice plates. It is found that values of pressure loss coefficient is a strong function of multi-hole diameter ratio, whereas, the flow conditioning properties are strongly affected by the number of holes.
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| 2. |
- Asim, Muhammad, et al.
(författare)
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Experimental analysis of solar thermal integrated MD system for cogeneration of drinking water and hot water for single family villa in Dubai using flat plate and evacuated tube solar collectors
- 2017
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Ingår i: Desalination and Water Treatment. - : DESALINATION PUBL. - 1944-3994 .- 1944-3986. ; 92, s. 46-59
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents the experimental analysis performed on solar thermal integrated membrane distillation (MD) system using flat plate and evacuated tube collectors. The system will be utilized for cogeneration of drinking water and domestic hot water for single family in Dubai comprising of four to five members. Experiments have been performed in Ras Al Khaimah Research and Innovation Centre (RAKRIC) facility. The experimental setup has been installed to achieve the required production of 15-25 L/d of drinking water and 250 L/d of hot water for domestic purposes. Experiments have been performed on MD setup at optimized flow rates of 6 L/min on hot side and 3 L/min on cold side for producing the desired distillate. The hot side and cold side MD temperature has been maintained between 60 degrees C and 70 degrees C, and 20 degrees C and 30 degrees C. The total annual energy demand comes out to be 8,223 kWh (6,000 kWh is for pure water and 2,223 kWh for hot water). The optimum aperture areas for flat plate and evacuated tube collector field have been identified as 8.5 and 7.5 m(2), respectively. Annual energy consumption per liter for pure water production is 1, 0.85 and 0.7 kWh/L for different MD hot and cold inlet temperatures.
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| 3. |
- Asim, Muhammad, et al.
(författare)
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Feasibility analysis of solar combi-system for simultaneous production of pure drinking water via membrane distillation and domestic hot water for single-family villa : pilot plant setup in Dubai
- 2016
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Ingår i: Desalination and Water Treatment. - : Taylor & Francis. - 1944-3994 .- 1944-3986. ; 57:46, s. 21674-21684
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents the feasibility study of installation of a solar-driven integrated MD desalination system for simultaneous production of pure drinking water and solar domestic hot water in United Arab Emirates (UAE) for a single-family villa comprising of 4-5 persons. In order to satisfy the current and future demand of water for domestic purposes, the desalination of seawater is considered to be one of the most effective and strategic technique in UAE. The stress on the underground water aquifers, rapid industrial growth, and increase in urban population in UAE results in the tremendous increase in fresh water demand during the past few decades. Since the local municipalities also provide the desalinated fresh water to the people but they mostly rely on bottled water for drinking purpose. In this paper, the pilot setup plant is designed, commissioned, and installed on site in UAE using air gap membrane distillation desalination process to fulfill the demand of 15-25 L/d of pure drinking water and 250 L/d of domestic hot water for a single-family villa. Experimental analyses have been performed on this setup during summer on flat plate solar collectors having different aperture areas (Experiments have been performed for aperture area of 11.9 m(2) in this research study for feasibility purpose). The average hot-side temperature ranges from 50 to 70 degrees C and average cold-side temperature of 35 degrees C.
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| 4. |
- Etcheverry, Sebastián, et al.
(författare)
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All fiber based micro-flow cytometer by combining optical fiber with inertial focusing
- 2016
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Ingår i: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016). - : Chemical and Biological Microsystems Society. - 9780979806490 ; , s. 1655-1656
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Konferensbidrag (refereegranskat)abstract
- Towards a portable point of care flow cytometry platform, we present here an integrated all optical fiber-based optofluidic system capable of counting and discriminating fluorescent particles and cells. The robust and compact device incorporates optical fibers and circular capillaries to build an all-fiber optofluidic device to enable counting particles based on their fluorescent and back-scatter light emission. Here, we combine this with inertial- and elasto-inertial microfluidics for sheathless particle and cell focusing for integrated detection with scattering and fluorescence detections - all necessary components of standard cytometers. We validated the system for cell counting based on scattering and fluorescence.
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| 5. |
- Etcheverry, Sebastián, et al.
(författare)
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High performance micro-flow cytometer based on optical fibres
- 2017
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 mu l/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.
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| 6. |
- Etcheverry, Sebastián, et al.
(författare)
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Optofludics in microstructured fibers combining particle elasto-inertial focusing and fluorescence
- 2016
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Ingår i: 2016 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO). - Washington, D.C. : IEEE conference proceedings. - 9781943580118
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Konferensbidrag (refereegranskat)abstract
- Optofluidics is exploited in an all-fiber component to detect and identify through fluorescence particles flowing at high rate and inertially focused in a capillary. The system represents a first step towards an in-fiber flow cytometer.
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| 7. |
- Faridi, Muhammad Asim
(författare)
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Bioparticle Manipulation using Acoustophoresis and Inertial Microfluidics
- 2017
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Despite the many promising advances made in microfluidics, sample preparation remains the single largest challenge and bottleneck in the field of miniaturised diagnostics. This thesis is focused on the development of sample preparation methods using active and passive particle manipulation techniques for point of care diagnostic applications. The active technique is based on acoustophoresis (acoustic manipulation) while the passive method is based on inertial microfluidics (hydrodynamic manipulation). In paper I, acoustic capillary-based cavity resonator was used to study aggregation of silica and polystyrene particles. We found that silica particles show faster aggregation time (5.5 times) and larger average area of aggregates (3.4 times) in comparison to polystyrene particles under the same actuation procedure. The silica particles were then used for acoustic based bacteria up-concentration. In paper II, a microfluidic-based microbubbles activated acoustic cell sorting technique was developed for affinity based cell separation. As a proof of principle, separation of cancer cell line in a suspension with better than 75% efficiency is demonstrated. For the passive sample preparation, inertial and elasto-inertial microfluidic approach that uses geometry-induced hydrodynamic forces for continuous size-based sorting of particles in a flow-through fashion were studied and applied for blood processing (paper III-V). In paper III, a simple ushaped curved channel was used for inertial microfluidics based enrichment of white blood cells from diluted whole blood. A filtration efficiency of 78% was achieved at a flow rate of 2.2 ml/min. In paper IV, elasto-inertial microfluidics where viscoelastic flow enables size-based migration of cells into a non- Newtonian solution, was used to continuously separate bacteria from unprocessed whole blood for sepsis diagnostics. Bacteria were continuously separated at an efficiency of 76% from undiluted whole blood sample. Finally, in paper V, the inertial and elasto-inertial techniques were combined with a detection platform to demonstrate an integrated miniaturized flow cytometer. The all-optical-fiber technology based system allows for simultaneous measurements of fluorescent and scattering data at 2500 particles/s. The use of inertial and acoustic techniques for sample preparation and development of an integrated detection platform may allow for further development and realization of point of care testing (POCT) systems.
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| 8. |
- Faridi, Muhammad Asim, et al.
(författare)
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Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics
- 2017
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Ingår i: Journal of Nanobiotechnology. - : BioMed Central (BMC). - 1477-3155. ; 15
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Tidskriftsartikel (refereegranskat)abstract
- Background: Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics.Results: In elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non- Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be sepa- rated. We first optimized the flow conditions using particles, and show continuous separation of 5 μm particles from 2 μm at a yield of 95% for 5 μm particle and 93% for 2 μm particles at respective outlets. Next, bacteria were continu- ously separated at an efficiency of 76% from undiluted whole blood sample.Conclusion: We demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria.
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| 9. |
- Faridi, Muhammad Asim, et al.
(författare)
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MicroBubble Activated Acoustic Cell Sorting : BAACS
- 2017
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Ingår i: Biomedical microdevices (Print). - : Springer. - 1387-2176 .- 1572-8781. ; 19:2
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Tidskriftsartikel (refereegranskat)abstract
- Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave. In acoustophoresis, cells being positive acoustic contrast particles migrate to pressure nodes. On the contrary we show that air-filled polymer-shelled microbubbles being strong negative acoustic contrast particles migrate to pressure antinodes at acoustic pressure amplitudes as low as 60 kPa. As a proof of principle, using the BAACS strategy, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency. Moreover, 100% of the microbubble-cell conjugates migrated to the anti-node. Hence a better upstream affinity-capture has the potential to provide higher sorting efficiency. The BAACS technique may potentially provide a simplistic approach for similar sized selective isolation of cells, and is suited for applications in point of care.
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| 10. |
- Faridi, Muhammad Asim, et al.
(författare)
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Milliliter scale acoustophoresis based bioparticle processing platform
- 2018
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Ingår i: ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018. - : ASME Press. - 9780791851197
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Konferensbidrag (refereegranskat)abstract
- Bioparticles such as mammalian cells and bacteria can be manipulated directly or indirectly for multiple applications such as sample preparation for diagnostic applications mainly up-concentration, enrichment & separation as well as immunoassay development. There are various active and passive microfluidic particle manipulation techniques where Acoustophoresis is a powerful technique showing high cell viability. The use of disposable glass capillaries for acoustophoresis, instead of cleanroom fabricated glass-silicon chip can potentially bring down the cost factor substantially, aiding the realization of this technique for real-world diagnostic devices. Unlike available chips and capillary-based microfluidic devices, we report milliliter-scale platform able to accommodate 1ml of a sample for acoustophoresis based processing on a market available glass capillary. Although it is presented as a generic platform but as a demonstration we have shown that polystyrene suspending medium sample can be processed with trapping efficiency of 87% and the up-concentration factor of 10 times in a flow through manner i.e., at 35µl/min. For stationary volume accommodation, this platform practically offers 50 times more sample handling capacity than most of the microfluidic setups. Furthermore, we have also shown that with diluted blood (0.6%) in a flow-through manner, 82% of the white blood cells (WBCs) per ml could be kept trapped. This milliliter platform could potentially be utilized for assisting in sample preparation, plasma separation as well as a flow-through immunoassay assay development for clinical diagnostic applications.
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