| 1. |
- Uhlén, Mathias, et al.
(författare)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Tidskriftsartikel (refereegranskat)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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| 2. |
- Asplund, Henrik, et al.
(författare)
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The COST 259 Directional Channel Model-Part II : Macrocells
- 2006
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Ingår i: IEEE Transactions on Wireless Communications. - 1536-1276 .- 1558-2248. ; 5:12, s. 3434-3450
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes the attributes of the COST 259 directional channel model that are applicable for use in the design and implementation of macrocellular mobile and portable radio systems and associated technology. Special care has been taken to model all propagation mechanisms that are currently understood to contribute to the characteristics of practical macrocellular channels and confirm that large scale, small scale, anddirectional characteristics of implemented models are realistic through their comparison with available measured data. The model that is described makes full use of previously published work, as well as incorporating some new results. It is considered that its implementation should contribute to a tool that can be used for simulations and comparison of different aspects of a large variety of wireless communication systems, including those that exploit the spatial aspects of radio channels, as, for example, throughthe use of adaptive antenna systems.
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| 3. |
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| 4. |
- Berglund, Lisa, et al.
(författare)
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A genecentric Human Protein Atlas for expression profiles based on antibodies
- 2008
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 7:10, s. 2019-2027
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Forskningsöversikt (refereegranskat)abstract
- An attractive path forward in proteomics is to experimentally annotate the human protein complement of the genome in a genecentric manner. Using antibodies, it might be possible to design protein-specific probes for a representative protein from every protein-coding gene and to subsequently use the antibodies for systematical analysis of cellular distribution and subcellular localization of proteins in normal and disease tissues. A new version (4.0) of the Human Protein Atlas has been developed in a genecentric manner with the inclusion of all human genes and splice variants predicted from genome efforts together with a visualization of each protein with characteristics such as predicted membrane regions, signal peptide, and protein domains and new plots showing the uniqueness (sequence similarity) of every fraction of each protein toward all other human proteins. The new version is based on tissue profiles generated from 6120 antibodies with more than five million immunohistochemistry-based images covering 5067 human genes, corresponding to approximately 25% of the human genome. Version 4.0 includes a putative list of members in various protein classes, both functional classes, such as kinases, transcription factors, G-protein-coupled receptors, etc., and project-related classes, such as candidate genes for cancer or cardiovascular diseases. The exact antigen sequence for the internally generated antibodies has also been released together with a visualization of the application-specific validation performed for each antibody, including a protein array assay, Western blot analysis, immunohistochemistry, and, for a large fraction, immunofluorescence-based confocal microscopy. New search functionalities have been added to allow complex queries regarding protein expression profiles, protein classes, and chromosome location. The new version of the protein atlas thus is a resource for many areas of biomedical research, including protein science and biomarker discovery.
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| 5. |
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| 6. |
- Harrysson, Fredrik, et al.
(författare)
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Dual antenna terminals in an indoor scenario
- 2006
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Ingår i: [Host publication title missing]. - 1550-2252. ; 6, s. 2737-2741
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Konferensbidrag (refereegranskat)abstract
- The performance of two different dual antenna hand-held test mobile terminals has been investigated in a realistic indoor office environment and scenario, with respect to antenna performance, diversity combining and Shannon MIMO channel capacity. Measurements of a 2times2 MIMO channel at 1877.5 MHz (narrowband) were performed using a dual-polarized base station antenna. Analyses show that diversity gains, using ideal selection combining and maximum ratio combining, between 0.7 and 4.6 dB was achieved at the 10% outage probability level. Ideal dual-side beamforming (single branch) gives up to 3.3 dB capacity gain compared to single antenna systems at SNR less than 10 dB, however, decreases with rising SNR. Dual branch MIMO capacity gain is only significant at higher SNR above 10 dB. In addition, horizontal polarization at the base station was found to outperform vertical polarization in this scenario
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| 7. |
- Molisch, Andreas, et al.
(författare)
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The COST 259 directional channel model - Part I: Overview and methodology
- 2006
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Ingår i: IEEE Transactions on Wireless Communications. - 1536-1276. ; 5:12, s. 3421-3433
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes a model for mobile radio channels that includes consideration of directions of arrival and is thus suitable for simulations of the performance of wireless systems that use smart antennas. The model is specified for 13 different types of environments, covering macro- micro- and picocells. In this paper, a hierarchy of modeling concepts is described, as well as implementation aspects that are valid for all environments. The model is based on the specification of directional channel impulse response functions, from which the impulse response functions at all antenna elements can be obtained. A layered approach, which distinguishes between external (fixed), large-scale-, and small-scale- parameters allows an efficient parameterization. Different implementation methods, based on either a tapped-delay line or a geometrical model, are described. The paper also derives the transformation between those two approaches. Finally, the concepts of clusters and visibility regions are used to account for large delay and angular spreads that have been measured. In two companion papers, the environment-specific values, of the model parameters are explained and justified.
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| 8. |
- Pontén, Fredrik, et al.
(författare)
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A global view of protein expression in human cells, tissues, and organs
- 2009
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Ingår i: Molecular Systems Biology. - : EMBO. - 1744-4292 .- 1744-4292. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- Defining the protein profiles of tissues and organs is critical to understanding the unique characteristics of the various cell types in the human body. In this study, we report on an anatomically comprehensive analysis of 4842 protein profiles in 48 human tissues and 45 human cell lines. A detailed analysis of over 2 million manually annotated, high-resolution, immunohistochemistry- based images showed a high fraction (>65%) of expressed proteins in most cells and tissues, with very few proteins (<2%) detected in any single cell type. Similarly, confocal microscopy in three human cell lines detected expression of more than 70% of the analyzed proteins. Despite this ubiquitous expression, hierarchical clustering analysis, based on global protein expression patterns, shows that the analyzed cells can be still subdivided into groups according to the current concepts of histology and cellular differentiation. This study suggests that tissue specificity is achieved by precise regulation of protein levels in space and time, and that different tissues in the body acquire their unique characteristics by controlling not which proteins are expressed but how much of each is produced. Molecular Systems Biology 5: 337; published online 22 December 2009; doi:10.1038/msb.2009.93
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| 9. |
- Riback, Mathias, et al.
(författare)
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Carrier frequency effects on path loss
- 2006
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Ingår i: [Host publication title missing]. - 1550-2252. ; 6, s. 2717-2721
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Konferensbidrag (refereegranskat)abstract
- To study the carrier frequency effects on path loss, measurements have been conducted at four discrete frequencies in the range 460-5100 MHz. The transmitter was placed on the roof of a 36 meters tall building and the receive antennas were placed on the roof of a van. Both urban and suburban areas were included in the measurement campaign. The results show that there is a frequency dependency, in addition to the well known free-space dependency 20 log10(f), in most of the areas included in the measurements. In non line of sight conditions, the excess path loss is clearly larger at the higher frequencies than at the lower. A model capturing these effects is presented
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