Sökning: WFRF:(Bajic Andrej)
> (2024) >
Physically and Chem...
Physically and Chemically Crosslinked Hyaluronic Acid-Based Hydrogels Differentially Promote Axonal Outgrowth from Neural Tissue Cultures
-
- Bajic, Andrej (författare)
- Uppsala universitet,Ortopedi och Handkirurgi
-
- Andersson, Brittmarie (författare)
- Uppsala universitet,Ortopedi och Handkirurgi
-
- Ossinger, Alexander (författare)
- Uppsala universitet,Ortopedi och Handkirurgi
-
visa fler...
-
- Tavakoli, Shima (författare)
- Uppsala universitet,Makromolekylär kemi
-
- Varghese, Oommen P., 1977- (författare)
- Uppsala universitet,Makromolekylär kemi
-
- Schizas, Nikos, 1979- (författare)
- Uppsala universitet,Ortopedi och Handkirurgi
-
visa färre...
-
(creator_code:org_t)
- MDPI, 2024
- 2024
- Engelska.
-
Ingår i: Biomimetics. - : MDPI. - 2313-7673. ; 9:3
- Relaterad länk:
-
https://doi.org/10.3...
-
visa fler...
-
https://uu.diva-port... (primary) (Raw object)
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
visa färre...
Abstract
Ämnesord
Stäng
- Our aim was to investigate axonal outgrowth from different tissue models on soft biomaterials based on hyaluronic acid (HA). We hypothesized that HA-based hydrogels differentially promote axonal outgrowth from different neural tissues. Spinal cord sliced cultures (SCSCs) and dorsal root ganglion cultures (DRGCs) were maintained on a collagen gel, a physically crosslinked HA-based hydrogel (Healon 5®) and a novel chemically crosslinked HA-based hydrogel, with or without the presence of neurotrophic factors (NF). Time-lapse microscopy was performed after two, five and eight days, where axonal outgrowth was assessed by automated image analysis. Neuroprotection was investigated by PCR. Outgrowth was observed in all groups; however, in the collagen group, it was scarce. At the middle timepoint, outgrowth from SCSCs was superior in both HA-based groups compared to collagen, regardless of the presence of NF. In DRGCs, the outgrowth in Healon 5® with NF was significantly higher compared to the rest of the groups. PCR revealed upregulation of NeuN gene expression in the HA-based groups compared to controls after excitotoxic injury. The differences in neurite outgrowth from the two different tissue models suggest that axons differentially respond to the two types of biomaterials.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- axonal outgrowth
- neuroprotection
- HA-based hydrogel
- spinal cord slice culture
- dorsal root ganglion culture
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas