| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- 2008
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Ingår i: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
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Forskningsöversikt (refereegranskat)abstract
- Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
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| 2. |
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| 3. |
- Ognissanto, F., et al.
(författare)
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Line integration effects on ion temperatures in tokamak plasmas measured with neutron emission spectroscopy
- 2008
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Ingår i: Review of Scientific Instruments. - : AIP Publishing. - 0034-6748 .- 1089-7623. ; 79:10, s. 10E511-
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Tidskriftsartikel (refereegranskat)abstract
- The line integrated line emission measured by neutron spectrometers at JET along sight lines in the vertical and horizontal planes has been simulated in Monte Carlo calculations to determine the relationship between the measured (effective) ion temperature (T-eff) relative to the peak value of the profile (T-0). The general sight line dependence of (T-eff) was expressed analytically for circular plasmas which was used to explain the simulated results for the actual JET sight lines. The analytical model with parametrization of sight lines and plasma profiles is described and the results are used to discuss dual sight line measurements that can be tested at JET and its forward implications for study burning (nearly thermal) plasmas of ITER.
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| 4. |
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| 5. |
- Gorini, G., et al.
(författare)
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Nuclear scattering effects on the neutron emission in fusion plasmas
- 2008
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Ingår i: Few-body systems. - : Springer Science and Business Media LLC. - 0177-7963 .- 1432-5411. ; 44:1-4, s. 57-60
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Tidskriftsartikel (refereegranskat)abstract
- Ion reactions and elastic scatterings in fusion plasmas of H and He nuclides are discussed as they can be observed in the neutron emission spectrum (NES). New calculations are presented on radio-frequency driven multi-step processes in (HeD)-He-3 plasmas involving knock-on scattering whose relative importance is discussed besides use for new plasma studies.
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| 6. |
- Hellesen, Carl, et al.
(författare)
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Validation of TRANSP Simulations Using Neutron emission Spectroscopy with Dual Sight Lines
- 2008
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Ingår i: Review of Scientific Instruments. - : AIP Publishing. - 0034-6748 .- 1089-7623. ; 79:10, s. 10E510-
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Tidskriftsartikel (refereegranskat)abstract
- A method to generate modeled neutron spectra from bulk and fast ion distributions simulated by TRANSP has been developed. In this paper, modeled data generated from fuel ion distrubutions modeled with TRANSP is compared to measured data from two neutron spectrometers with different lines of sight; TOFOR with a radial one and the MPRu with a tangential one. The information obtained from the analysis of the measured neutron spectra such as the relative intensity of the emission from different ion populations places additional constraints on the simulation and can be used to adjust the parameters of the simulation.
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| 7. |
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