Sökning: WFRF:(Ballmann Mónika Z.)
> (2018) >
Generation and char...
Generation and characterization of a novel candidate gene therapy and vaccination vector based on human species D adenovirus type 56
-
Duffy, Margaret R. (författare)
-
Alonso-Padilla, Julio (författare)
-
- Lijo, John, 1979- (författare)
- Umeå universitet,Virologi,Niklas Arnberg
-
visa fler...
-
- Chandra, Naresh, 1987- (författare)
- Umeå universitet,Virologi,Niklas Arnberg
-
Khan, Selina (författare)
-
Ballmann, Monika Z. (författare)
-
Lipiec, Agnieszka (författare)
-
Heemskerk, Evert (författare)
-
Custers, Jerome (författare)
-
- Arnberg, Niklas, 1969- (författare)
- Umeå universitet,Virologi,Niklas Arnberg
-
Havenga, Menzo (författare)
-
Baker, Andrew H. (författare)
-
Lemckert, Angelique (författare)
-
visa färre...
-
(creator_code:org_t)
- Microbiology Society, 2018
- 2018
- Engelska.
-
Ingår i: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 99, s. 135-147
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://umu.diva-por... (primary) (Raw object)
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- The vectorization of rare human adenovirus (HAdV) types will widen our knowledge of this family and their interaction with cells, tissues and organs. In this study we focus on HAdV-56, a member of human Ad species D, and create ease-of-use cloning systems to generate recombinant HAdV-56 vectors carrying foreign genes. We present in vitro transduction profiles for HAdV-56 in direct comparison to the most commonly used HAdV-5-based vector. In vivo characterizations demonstrate that when it is delivered intravenously (i.v.) HAdV-56 mainly targets the spleen and, to a lesser extent, the lungs, whilst largely bypassing liver transduction in mice. HAdV-56 triggered robust inflammatory and cellular immune responses, with higher induction of IFNγ, TNFα, IL5, IL6, IP10, MCP1 and MIG1 compared to HAdV-5 following i.v. administration. We also investigated its potential as a vaccine vector candidate by performing prime immunizations in mice with HAdV-56 encoding luciferase (HAdV-56-Luc). Direct comparisons were made to HAdV-26, a highly potent human vaccine vector currently in phase II clinical trials. HAdV-56-Luc induced luciferase 'antigen'-specific IFNγ-producing cells and anti-HAdV-56 neutralizing antibodies in Balb/c mice, demonstrating a near identical profile to that of HAdV-26. Taken together, the data presented provides further insight into human Ad receptor/co-receptor usage, and the first report on HAdV-56 vectors and their potential for gene therapy and vaccine applications.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- adenovirus
- HAdV-56
- gene therapy
- vaccine vector
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Duffy, Margaret ...
-
Alonso-Padilla, ...
-
Lijo, John, 1979 ...
-
Chandra, Naresh, ...
-
Khan, Selina
-
Ballmann, Monika ...
-
visa fler...
-
Lipiec, Agnieszk ...
-
Heemskerk, Evert
-
Custers, Jerome
-
Arnberg, Niklas, ...
-
Havenga, Menzo
-
Baker, Andrew H.
-
Lemckert, Angeli ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Mikrobiologi ino ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Medicinsk biotek ...
- Artiklar i publikationen
-
Journal of Gener ...
- Av lärosätet
-
Umeå universitet