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- Belfrage, Per, et al.
(författare)
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Alterations of lipid metabolism in healthy volunteers during long-term ethanol intake
- 1977
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Ingår i: European Journal of Clinical Investigation. - : Wiley. - 0014-2972 .- 1365-2362. ; 7:2, s. 127-131
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Tidskriftsartikel (refereegranskat)abstract
- Nine young, healthy male volunteers were given ethanol (75 g/day) for 5 weeks. The ethanol was divided into five daily doses and taken so that blood ethanol levels never exceeded 0.04% (w/v). During the latter part of the ethanol intake period, there was a significant, transient increase of plasma triglyceride (TG) concentrations followed by reduction to normal levels. A three-fold increase of lipoprotein lipase activity (LLA) occurred in biopsy specimens of adipose tissue. An increase of alpha-lipoprotein concentrations, which correlated significantly with the decrease in plasma TG levels and the increase in adipose LLA, was also observed during the ethanol intake period. No changes were observed in plasma cholesterol and beta-lipoprotein levels. A transient, three-fold increase of TG concentrations occurred in liver biopsy specimens. Ultrastructural and cytochemical examinations of the biopsy specimens showed hyperplasia of the smooth endoplasmic reticulum, and increased canallicular activity of gamma-glutamyl transferase (gamma-GT) activity in most subjects towards the end of and after the ethanol intake period. Serum gamma-GT levels also increased significantly.
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| 2. |
- Belfrage, Per, et al.
(författare)
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Dispersion of viable pig liver cells with collagenase
- 1975
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Ingår i: Life Sciences. - : Elsevier BV. - 1879-0631 .- 0024-3205. ; 17:8, s. 1219-1225
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Tidskriftsartikel (refereegranskat)abstract
- Viable suspended hepatocytes were prepared from surgical biopsy specimens of pig and human liver by digestion with collagenase. Initial perfusion of the tissue through cannulated blood vessels with 0.5 mM EGTA followed by 0.2% collagenase gave the best results. 20−870 × 106 cells of which 60–95 % excluded trypan blue were obtained from 5–30 g pig liver pieces, while results with human liver specimens were usually less satisfactory. In some experiments, however, viable cells, as judged by vital stain exclusion and ability to synthesize lipids were obtained in sufficient yield. In the pig hepatocytes glycerolipid synthesis from [3H] glycerol and oxidation and esterification of [14C] oleic acid had the same characteristics as those observed earlier in rat hepatocytes.
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| 3. |
- Nilsson-Ehle, Peter, et al.
(författare)
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Effects of ethanol intake on lipoprotein lipase activity in adipose tissue of fasting subjects
- 1978
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Ingår i: Lipids. - 0024-4201. ; 13:6, s. 433-437
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Tidskriftsartikel (refereegranskat)abstract
- Ethanol (ca. 1 g/kg body weight) was given alone or together with glucose or lipid (mixed triglycerides) perorally to young, fasting subjects. The changes with time (0-6 hr) of lipoprotein lipase activity (LLA) in adipose tissue, plasma glycerol, triglyceride, insulin, blood glucose, and alcohol concentrations were followed. A maximal mean blood alcohol concentration of 0.09% (w/v) was obtained 1 hr after ingestion with no apparent intoxicating effects. Ethanol intake prevented the previously observed [Nilsson-Ehle, P., S. Carlstrom, and P. Belfrage, Scand, J. Clin. Lab. Invest. 35:373 (1975)] glucose-induced rapid elevation of adipose tissue LLA but had small effects on this enzymatic activity when given alone or together with lipid. Confirming results by others, ethanol intake decreased plasma glycerol concentration and increased plasma triglycerides, especially after intake of lipid. It is suggested that ethanol intake interferes with the normal carbohydrate-induced elevation of adipose tissue LLA after a mixed meal, thereby decreasing the removal capacity for circulating dietary lipid and causing enhanced and prolonged alimentary hyperlipemia.
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