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- Andreasson, Ulrika, et al.
(författare)
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Identification of molecular targets associated with transformed diffuse large B cell lymphoma using highly purified tumor cells
- 2009
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Ingår i: American Journal of Hematology. - : Wiley. - 0361-8609 .- 1096-8652. ; 84:12, s. 803-808
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Tidskriftsartikel (refereegranskat)abstract
- Follicular lymphoma (FL) frequently transforms into the more aggressive diffuse large B cell lymphoma (DLBCL-tr), but no protein biomarkers have been identified for predictive or early diagnosis. Gene expression analyses have identified genes changing on transformation but have failed to be reproducible in different studies, reflecting the heterogeneity within the tumor tissue and between tumor samples. Gene expression analyses on Affymetrix Human Genome U133 Plus 2.0 arrays were performed, using flow cytometry sorted tumor cells derived from FL and transformed DLBCL. To identify molecular targets associated with the transformation, subsequent immunohistochemistry (IHC) analyses of the corresponding proteins were performed. Using highly purified cells, this study identified 163 genes, which were significantly deregulated during the transformation in a majority of cases. Among the upregulated transcripts, 13 genes were selected for validation using IHC, based on the availability of commercial antibodies, and galectin-3 and NEK2 proteins specifically identify DLBCL-tr, when compared with FL. We demonstrate that by purifying tumor cells through cell sorting, thereby reducing the heterogeneity due to infiltrating cells, it was possible to identify distinct differences between tumor entities rather than variations due to cellular composition. Galectin-3 and NEK2 both identified a subgroup of DLBCL-tr, and the function of these protein markers also suggests a biological role in the transformation process.
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| 6. |
- Berglund, Sara
(författare)
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Vägen till vinnarcirkeln. Travhästen och dess människor mellan sport och spel
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The way we understand and treat our horses, like the purpose for which they are bred, is culturally conditioned. The transition between differing social formations involves not only new life circumstances for people and animals, but also changed social modes of relating between them. Certain of the animals? previous functions and types of use come to an end at the same time new ones arise in their place. In this dissertation, the focus is on harness racing, where the meaning and function of the horse over the years have been subject to renegotiation. The purpose is to examine how the trotting horse is made, in relation to the conceptions, expectations and requirements of the sport. The making of the trotting horse is both social and physical; that is, it is manifested in the understanding of the animal as well as in its physicality. This process coincided with the organisation of the sport. Then as now, control was exercised over when, how and which individuals were allowed to reproduce. The work of breeding has fashioned trotting horses that are faster as well as built more for man's specific purposes than their predecessors. Physically and mentally they have been adapted to the racing form and the high speeds. Horses that enter race courses today differ in physiognomy and gait pattern not only from trotting horses of older times, but also, for example, from riding- and race horses. When the purpose of keeping horses changed, new groups of people became associated with the enterprise, such as gamblers, professional trainers and drivers, all of whom in their own ways affect the way the trotting horse is understood and made.
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| 7. |
- Ellmark, Peter, et al.
(författare)
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Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays
- 2008
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Ingår i: Cancer Letters. - : Elsevier BV. - 0304-3835 .- 1872-7980. ; 265:1, s. 98-106
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Tidskriftsartikel (refereegranskat)abstract
- Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan (TM) antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naive B cells.
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| 8. |
- Eriksson, Mikael, et al.
(författare)
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MAX-IV Design: Pushing the Envelope
- 2007
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Ingår i: 2007 IEEE Particle Accelerator Conference, vols 1-11. - 9781424409167 ; , s. 1277-1279
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Konferensbidrag (refereegranskat)abstract
- The proposed MAX IV facility is meant as a successor to the existing MAX-lab. The accelerator part will consist of three storage rings, two new ones operated at 3 and 1.5 GeV respectively and the existing 700 MeV MAX III ring. The two new rings have identical lattices and are placed on top of each other. Both these rings have a very small emittance, 0.86 and 0.4 nm rad respectively, and offer synchrotron radiation of very high mean brilliance. As an injector, a 3 GeV linear accelerator is planned. The design philosophy and the special technical solutions called for are presented in this paper.
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| 9. |
- Eriksson, Mikael, et al.
(författare)
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STATUS OF THE MAX IV LIGHT SOURCE PROJECT
- 2006
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Ingår i: European Particle Accelerator Conference 2006, Edinburgh, UK.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Jarefors, Sara, et al.
(författare)
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Lyme borreliosis reinfection: might it be explained by gender difference in immune response?
- 2006
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Ingår i: Immunology. - : Wiley. - 0019-2805 .- 1365-2567. ; 118:2, s. 224-235
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Tidskriftsartikel (refereegranskat)abstract
- Lyme borreliosis is a tick-borne disease often manifesting as a circular skin lesion. This cutaneous form of the disease is known as erythema migrans. In a 5-year follow-up study in southern Sweden, 31 of 708 individuals initially diagnosed with erythema migrans and treated with antibiotics were found to be reinfected with Borrelia burgdorferi. Although men and women were tick-bitten to the same extent, 27 of the 31 reinfected individuals were women, all of whom were over 44 years of age. The aim of this study was to determine whether this discrepancy in gender distribution could be a result of differences in immunological response. Twenty single-infected and 21 reinfected women and 18 single-infected and three reinfected men were included in the study. None of the participants showed any sign of an ongoing B. burgdorferi infection, and thus the habitual response was captured. Lymphocytes were separated from blood and stimulated with antigens. The secretion of interleukin (IL)-4, IL-6, IL-10, interferon (IFN)-γ and tumour necrosis factor (TNF)-α was measured by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunosorbent spot-forming cell assay (ELISPOT) or Immulite. No difference was detected in cytokine secretion between single-infected and reinfected individuals. We also compared the immunological response in men and women, regardless of the number of B. burgdorferi infections. Women displayed a significantly higher spontaneous secretion of all cytokines measured. The ratios of IL-4:IFN-γ and IL-10:TNF-α were significantly higher in women. Gender differences in immune reactivity might in part explain the higher incidence of reinfection in women. The higher IL-4:IFN-γ and IL-10:TNF-α ratios seen in women indicate that postmenopausal women have T helper type 2 (Th2)-directed reactivity with impaired inflammatory responses which might inhibit the elimination of spirochetes.
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