| 1. |
|
|
| 2. |
- Thörn, Ingrid, 1957-, et al.
(författare)
-
Minimal residual disease assessment in childhood acute lymphoblastic leukemia : Results of a Swedish multi-centre study comparing real-time PCR and multi-colour flow cytometry
- 2009
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- In this Swedish multi-center study of early treatment response in childhood acute lymphoblastic leukemia (ALL), we evaluated the concordance between multicolour flow cytometry (FCM) and real-time quantitative polymerase chain reaction (RQ-PCR) for assessment of minimal residual disease (MRD). Multiple time points (i.e. day 15, 29, 50 and 106) were evaluated with the NOPHO (Nordic Society of Pediatric Hematology and Oncology) ALL 2000 treatment protocol as backbone. During 2002-2006, 334 children were diagnosed with ALL, where 228 had paired samples taken at any of the four time points. With the detection level of 0.1%, the concordance between RQ-PCR and FCM was 90% in the 726 paired samples analyzed. At day 29, the correlation between the methods was greater with MRD levels >0.1% (rs=0.7, p<0.001) than below (rs=0.2, p=0.024). MRD levels higher than 0.1% at day 29 was a significant predictor of higher risk of having a bone marrow relapse. This was true both for BCP ALL and T-ALL analysed with either FCM or RQ-PCR, although RQ-PCR was a better discriminator than FCM in T-ALL. However, using the NOPHO ALL 2000 protocol, our data indicate that a higher cut-off value (0.2%) should be applied in BCP ALL when using RQ-PCR as MRD method. In contrast, MRD levels ≥ 0.1%, analysed with either method late during induction therapy, was not a predictor of isolated extramedullary relapse. We therefore conclude that MRD assessment by RQ-PCR based IG/TCR rearrangement and multicolour FCM monitoring can be used as a clinical tool if the aim is to find childhood ALL cases with increased risk of having bone marrow relapses.
|
|
| 3. |
|
|
| 4. |
- Andersson, Sören, et al.
(författare)
-
Frictions models for sliding dry, boundary and mixed lubricated contacts
- 2007
-
Ingår i: Tribology International. - : Elsevier BV. - 0301-679X .- 1879-2464. ; 40, s. 580-587
-
Tidskriftsartikel (refereegranskat)abstract
- Friction, lubrication, and wear have a strong influence on the performance and behavior of mechanical systems. This paper deals with different friction models for sliding contacts running under different conditions. The models presented are suited to different situations, depending on the type of contact, running conditions, and the behavior of interest. The models will be discussed from simulation and tribological points of view. The different types of friction models considered are:center dot friction models for transient sliding under dry, boundary and mixed lubrication conditions,center dot friction models for micro-displacements of engineering surfaces subjected to transient sliding,center dot friction models often used in the simulation and control of technical systems,center dot combined friction models that represent physical behaviors fairly well but are also suitable for use in simulating systems,center dot friction models that take into account the stochastic nature of interacting surface asperities
|
|
| 5. |
- Balciunas, Darius, et al.
(författare)
-
Functional interactions within yeast mediator and evidence of differential subunit modifications
- 2003
-
Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 278:6, s. 3831-3839
-
Tidskriftsartikel (refereegranskat)abstract
- It is possible to recruit RNA polymerase II to a target promoter and, thus, activate transcription by fusing Mediator subunits to a DNA binding domain. To investigate functional interactions within Mediator, we have tested such fusions of the lexA DNA binding domain to Med1, Med2, Gal11, Srb7, and Srb10 in wild type, med1, med2, gal11, sin4, srb8, srb10, and srb11 strains. We found that lexA-Med2 and lexA-Gal11 are strong activators that are independent of all Mediator subunits tested. lexA-Srb10 is a weak activator that depends on Srb8 and Srb11. lexA-Med1 and lexA-Srb7 are both cryptic activators that become active in the absence of Srb8, Srb10, Srb11, or Sin4. An unexpected finding was that lexA-VP16 differs from Gal4-VP16 in that it is independent of the activator binding Mediator module. Both lexA-Med1 and lexA-Srb7 are stably associated with Med4 and Med8, which suggests that they are incorporated into Mediator. Med4 and Med8 exist in two mobility forms that differ in their association with lexA-Med1 and lexA-Srb7. Within purified Mediator, Med4 is present as a phosphorylated lower mobility form. Taken together, these results suggest that assembly of Mediator is a multistep process that involves conversion of both Med4 and Med8 to their low mobility forms.
|
|
| 6. |
|
|
| 7. |
- Björklund, Elisabet, et al.
(författare)
-
Quality control of flow cytometry data analysis for evaluation of minimal residual disease in bone marrow from acute leukemia patients during treatment.
- 2009
-
Ingår i: Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. - : Lippincott Williams & Wilkins. - 1536-3678 .- 1077-4114. ; 31:6, s. 406-15
-
Tidskriftsartikel (refereegranskat)abstract
- Low levels of leukemia cells in the bone marrow, minimal residual disease (MRD), are considered to be a powerful indicator of treatment response in acute lymphatic leukemia (ALL). A Nordic quality assurance program, aimed on standardization of the flow cytometry MRD analysis, has been established before implementation of MRD at cutoff level 10 as one of stratifying parameters in next Nordic Society of Pediatric Hematology and Oncology (NOPHO) treatment program for ALL. In 4 quality control (QC) rounds 15 laboratories determined the MRD levels in 48 follow-up samples from 12 ALL patients treated according to NOPHO 2000. Analysis procedures were standardized. For each QC round a compact disc containing data in list-mode files was sent out and results were submitted to a central laboratory. At cutoff level 10, which will be applied for clinical decisions, laboratories obtained a high concordance (91.6%). If cutoff level 10 was applied, the concordance would be lower (85.3%). The continuing standardization resulted in better concordance in QC3 and QC4 compared with QC1 and QC2. The concordance was higher in precursor B as compared with T-cell ALL. We conclude that after standardization, flow cytometry MRD detection can be reliably applied in international, multicenter treatment protocols.
|
|
| 8. |
- Björklund, Stefan, et al.
(författare)
-
Compensation of systematic errors in five-axis high-speed machining
- 2002
-
Ingår i: International Journal of Production Research. - : Informa UK Limited. - 0020-7543 .- 1366-588X. ; 40:15 SPEC., s. 3765-3778
-
Tidskriftsartikel (refereegranskat)abstract
- A method is described for the compensation of errors associated with tool path generation, particularly during five-axis high-speed machining (HSM). Information on machine tool performance and its dynamic features is used to calculate possible errors and convenient modifications of the NC program, thereby avoiding errors when parts are actually being machined. This 'preprocess method' by means of postprocessing with NC software is presented. The errors dealt with are mainly servo lag errors, but the explored approach can support most systematic errors associated with machine tool performance. These are briefly summarized. Research so far has largely been aimed at the implementation of compensation routines in the CNC controller and at corrections in real time. The problem is that most applications are only available for three-axis milling. The presented approach (compensation before machining by using software routines in a specially designed postprocessor) is based on a fuzzy logic expert system. The benefits can be summarized as data reduction, data improvement, precontrol of feed, and improved component accuracy. The applied procedure is also convenient for implementation in an industrial environment by retrofitting existing equipment. The suggested method provides improved control over machine dynamics, permitting high-speed machining centres to maintain a maximum or near-maximum feed rate despite axis reversals and tool path changes, even at corners. Two categories of results are presented, namely the management of NC data related to expected performance of the machine tool and improvements of the machine tool performance in terms of productivity and accuracy of the machined test components.
|
|
| 9. |
- Björklund, Stefan
(författare)
-
Reformismens anatomi : Erasmus versus Luther
- 2009
-
Bok (övrigt vetenskapligt/konstnärligt)abstract
- The author analyses the sharp philosophical debates between Erasmus Rotterdamus and Martin Luther, revolutionary and determinist, and takes that as a ground for discussion of the concept of "reformism". Ha proposes that "reformism" should [sic] used for a "step-by-step" method of political change, irrespective of the content of policies. He also tries to list some characteristics of the "reformistic" attitude.
|
|
| 10. |
|
|
