Sökning: WFRF:(Bojmar Linda 1983 )
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Extracellular vesic...
Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids
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- Bojmar, Linda, 1983- (författare)
- Linköpings universitet,Avdelningen för kirurgi, ortopedi och onkologi,Medicinska fakulteten,Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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- Kim, Han Sang (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea
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- Tobias, Gabriel C (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil
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- Pelissier Vatter, Fanny A (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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- Lucotti, Serena (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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- Gyan, Kofi Ennu (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Tri-Institutional PhD Program in Computational Biology and Medicine, New York, NY, USA
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- Kenific, Candia M. (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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- Wan, Zurong (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; Department of Pediatrics, Weill Cornell Medicine, New York, NY, USA
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- Kim, Kyung-A (författare)
- Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea
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- Kim, DooA (författare)
- Yonsei Cancer Center, Division of Medical Oncology, Department of Internal Medicine, Graduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, Korea
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- Hernandez, Jonathan (författare)
- Hepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Surgical Oncology Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA
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- Pascual, Virginia (författare)
- Department of Pediatrics, Weill Cornell Medicine, New York, NY, USA; Drukier Institute for Childrens Health, Weill Cornell Medicine, New York, NY, USA
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- Heaton, Todd E (författare)
- Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
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- La Quaglia, Michael P (författare)
- Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Pediatric Surgical Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
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- Kelsen, David (författare)
- Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA
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- Trippett, Tanya M (författare)
- Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA
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- Jones, David R (författare)
- Thoracic Surgery Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
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- Jarnagin, William R. (författare)
- Hepatopancreatobiliary Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA
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- Matei, Irina R. (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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- Zhang, Haiying (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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- Hoshino, Ayuko (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan
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- Lyden, David (författare)
- Childrens Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Childrens Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA
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(creator_code:org_t)
- Cell Press, 2021
- 2021
- Engelska.
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Ingår i: STAR protocols. - : Cell Press. - 2666-1667. ; 2:1
- Relaterad länk:
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https://doi.org/10.1...
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https://liu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
Nyckelord
- Cell Membrane; Cell culture; Cell isolation; Exomeres; Exosomes; Extracellular vesicles and particles; Isolation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Bojmar, Linda, 1 ...
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Kim, Han Sang
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Tobias, Gabriel ...
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Pelissier Vatter ...
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Lucotti, Serena
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Gyan, Kofi Ennu
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visa fler...
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Kenific, Candia ...
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Wan, Zurong
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Kim, Kyung-A
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Kim, DooA
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Hernandez, Jonat ...
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Pascual, Virgini ...
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Heaton, Todd E
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La Quaglia, Mich ...
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Kelsen, David
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Trippett, Tanya ...
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Jones, David R
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Jarnagin, Willia ...
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Matei, Irina R.
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Zhang, Haiying
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Hoshino, Ayuko
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Lyden, David
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Biomedicinsk lab ...
- Artiklar i publikationen
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STAR protocols
- Av lärosätet
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Linköpings universitet