| 1. |
- Andersson, Gunilla, et al.
(författare)
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The effect of Swedish and American smokeless tobacco extract on periodontal ligament fibroblasts in vitro
- 2006
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Ingår i: Swedish Dental Journal. - : Swedish Dental Association. - 0347-9994. ; 30:3, s. 89-97
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Tidskriftsartikel (refereegranskat)abstract
- Use of moist snuff is widespread in Sweden. In 2004 approximately 8oo,ooo Swedes were daily users which corresponds to 22% of the male population and 3% of the female population. The aim of the present study was to evaluate the effect of Swedish moist snuff extract on PDLfibroblast growth and hard tissue production and compare with moist snuff extract from USA. Periodontal ligament cells (PDL-cells) were obtained from 3 healthy subjects (1 female 14 years, 2 males 14 and 17 years) from the root surface of premolars extracted for orthodontic reasons. The cells were isolated from explants and grown in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum (FBS) and cultivated in 37 degrees C with 5% CO2 in air. Snuff extract in concentrations 0.3%, 1% and 3% (in DMEM with 1% FBS) was tested. Cells from each individual were tested three times, each time in triplicate. Photographs were taken at o and 24 hours with a digital camera and analysed in terms of growth and morphology. Then the cell suspension was frozen and later thawed for examination of the production of alkaline phosphatase after exposure to different snuff concentrations. This in vitro study has shown that PDL cells from 3 different subjects demonstrated a reduced number of cells at exposure to 3% of both Swedish and American snuff extract.The production of alkaline phosphatase after 2 hours was similarly reduced from cells exposed to 3% snuff extract. Further studies have to be made to understand the effect of smokeless tobacco on periodontal tissues. However, from this study can be concluded that smokeless tobacco has biological effects in terms of reduced PDL cell growth and production of alkaline phosphatase
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| 2. |
- Jonsson, D, et al.
(författare)
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Functional significance of female sex hormone receptors in the periodontium (Dublin)
- 2006
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Konferensbidrag (refereegranskat)abstract
- Objectives: Several studies have addressed the association between changes in estrogen and progesterone levels and changes in parameters of periodontitis. The purpose of this project is to investigate the mechanisms by which estrogen influence structure and function of the periodontal ligament by affecting the properties of periodontal ligament cells (PDL cells). Methods: PDL cells were obtained from teeth extracted for orthodontic reasons. The cells were cultured from periodontal tissue explants and used in passages 3-5. Estrogen (ER) expression was investigated by immunocytochemistry. Subcellular distribution of ERβ was determined using mitotracker. Expression of mitochondrial proteins was done using Western blotting. DNA and collagen synthesis was measured using incorporation of radioactive isotopes. Cytokine expression was investigated using ELISA. Results: ERβ but not ERα immunoreactivity was observed in the PDL cells. Preliminary results show that ERβ is distributed not only in the nucleus but also in the mitochondria. To study the effect of mitochondrial ERβ we will investigate synthesis of mitochondrial proteins. Estrogen increased DNA synthesis in human breast cancer cells but had no effect on PDL cell DNA or collagen synthesis. Conclusion: Human PDL cells express ERβ suggesting that estrogen affects PDL cellular function via this ER subtype. Estrogen has no effect on DNA and collagen synthesis, showing that estrogen has no beneficial effect on the periodontium via this mechanism. Instead estrogen via ERβ acts on the periodontium via another still unknown mechanism, perhaps via mitochondrial function or regulatory effect on cytokines.
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| 3. |
- Jonsson, D, et al.
(författare)
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Functional significance of female sex hormone receptors in the periodontium (Madrid)
- 2006
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Konferensbidrag (refereegranskat)abstract
- Department of Experimental Medical Sciences, Lund University, Sweden. Department of Periodontology, Faculty of Odontology, Malmö University, Sweden. Introduction: Several studies have addressed the association between changes in estrogen and progesterone levels and changes in parameters of periodontitis. The purpose of this project is to investigate the mechanisms by which estrogen influence structure and function of the periodontal ligament by affecting the properties of periodontal ligament cells (PDL cells). Materials and methods: PDL cells were obtained from teeth extracted for orthodontic reasons. The cells were cultured from periodontal tissue explants and used in passages 3-5. Estrogen (ER) and progesterone receptor (PR) expression was investigated by immunocytochemistry. Subcellular distribution of ERβ was determined using mitotracker, immunogoldlabeling and electronmicroscopy. DNA and collagen synthesis was measured using incorporation of radioactive isotopes. Results: ERβ but not ERα or PR immunoreactivity was observed in the PDL cells. Preliminary results show that ERβ is distributed not only in the nucleus but also in the mitochondria and cytosol. Estrogen increased DNA synthesis in human breast cancer cells but had no effect on PDL cell DNA or collagen synthesis. Conclusions: Human PDL cells express ERβ suggesting that estrogen affects PDL cellular function via this ER subtype. Estrogen has no effect on DNA and collagen synthesis, showing that estrogen has no beneficial effect on the periodontium via this mechanism. Instead estrogen via ERβ acts on the periodontium via another still unknown mechanism.
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