| 1. |
- Cako, Stefan, 1994-
(author)
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The Challenges of Russian Militarization in Outer Space
- 2020
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In: Kungl Krigsvetenskapsakademiens Handlingar och Tidskrift. - 0023-5369. ; :3, s. 149-162
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Journal article (other academic/artistic)abstract
- Den ständigt växande användningen av satelliter från USA har även lett till ökat beroende avdem för viktiga områden som täcker militär underrättelsetjänst liksom allt fler civila områden.Detta har inte gått obemärkt förbi, då samarbetet mellan Ryssland och Kina intensifieras genomatt samla ihop resurser och vetenskaplig expertis för och kunna undergräva och utnyttjadessa tydliga sårbarheter i rymden. Samtidigt som de framgångsrikt för fram ett effektivt narrativom att stödja fred i rymden och icke-militarisering. Artikeln utvärderar det ryska hoteti rymden i skenet av den ökande användningen av ryska gråzonsoperationer och sårbarheterför amerikanska och andra aktörers satelliter. Således sårbarheten för just sådana aktivitetervilka inom en snar framtid kommer att omfattas av att allt fler diffusa aktörer kommer attnyttja rymden i samband med pågående kommersialisering av densamma.
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| 2. |
- Vorobyeva, Anzhelika, et al.
(author)
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Investigation of a Pharmacological Approach for Reduction of Renal Uptake of Radiolabeled ADAPT Scaffold Protein
- 2020
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In: Molecules. - : MDPI. - 1431-5157 .- 1420-3049. ; 25:19
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Journal article (peer-reviewed)abstract
- Albumin binding domain-Derived Affinity ProTeins (ADAPTs) are small (5 kDa) engineered scaffold proteins that are promising targeting agents for radionuclide-based imaging. A recent clinical study has demonstrated that radiolabeled ADAPTs can efficiently visualize human epidermal growth factor receptor 2 (HER2) expression in breast cancer using SPECT imaging. However, the use of ADAPTs directly labeled with radiometals for targeted radionuclide therapy is limited by their high reabsorption and prolonged retention of activity in kidneys. In this study, we investigated whether a co-injection of lysine or gelofusin, commonly used for reduction of renal uptake of radiolabeled peptides in clinics, would reduce the renal uptake of [Tc-99m]Tc(CO)(3)-ADAPT6 in NMRI mice. In order to better understand the mechanism behind the reabsorption of [Tc-99m]Tc(CO)(3)-ADAPT6, we included several compounds that act on various parts of the reabsorption system in kidneys. Administration of gelofusine, lysine, probenecid, furosemide, mannitol, or colchicine did not change the uptake of [Tc-99m]Tc(CO)(3)-ADAPT6 in kidneys. Sodium maleate reduced the uptake of [Tc-99m]Tc(CO)(3)-ADAPT6 to ca. 25% of the uptake in the control, a high dose of fructose (50 mmol/kg) reduced the uptake by ca. two-fold. However, a lower dose (20 mmol/kg) had no effect. These results indicate that common clinical strategies are not effective for reduction of kidney uptake of [Tc-99m]Tc(CO)(3)-ADAPT6 and that other strategies for reduction of activity uptake or retention in kidneys should be investigated for ADAPT6.
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| 3. |
- Vorobyeva, Anzhelika, et al.
(author)
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Optimal composition and position of histidine-containing tags improves biodistribution of Tc-99m-labeled DARP in G3
- 2019
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In: Scientific Reports. - : NATURE PUBLISHING GROUP. - 2045-2322. ; 9
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Journal article (peer-reviewed)abstract
- Radionuclide molecular imaging of HER2 expression in disseminated cancer enables stratification of patients for HER2-targeted therapies. DARP in G3, a small (14 kDa) engineered scaffold protein, is a promising probe for imaging of HER2. We hypothesized that position (C- or N-terminus) and composition (hexahistidine or (HE)(3)) of histidine-containing tags would influence the biodistribution of [Tc-99m]Tc(CO)(3)-labeled DARP in G3. To test the hypothesis, G3 variants containing tags at N-terminus (H-6-G3 and (HE)(3)-G3) or at C-terminus (G3-H-6 and G3-(HE)(3)) were labeled with [Tc-99m]Tc(CO)(3). Labeling yield, label stability, specificity and affinity of the binding to HER2, biodistribution and tumor targeting properties of these variants were compared side-by-side. There was no substantial influence of position and composition of the tags on binding of [Tc-99m]Tc(CO)(3)-labeled variants to HER2. The specificity of HER2 targeting in vivo was confirmed. The tumor uptake in BALB/c nu/nu mice bearing SKOV3 xenografts was similar for all variants. On the opposite, there was a strong influence of the tags on uptake in normal tissues. The tumor-to-liver ratio for [Tc-99m]Tc(CO)(3)-(HE)(3)-G3 was three-fold higher compared to the hexahistidine-tag containing variants. Overall, [Tc-99m]Tc(CO)(3)-(HE)(3)-G3 variant provided the highest tumor-to-lung, tumor-to-liver, tumor-to-bone and tumor-to-muscle ratios, which should improve sensitivity of HER2 imaging in these common metastatic sites.
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| 4. |
- Murphy, Lauren L., et al.
(author)
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An Hedonomic Evaluation of the Effect of Repeated System-Exposure on Pleasurable Human-System Experience
- 2008
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In: Proceedings of the Human Factors and Ergonomics Society Annual Meeting September 2008 vol. 52 no. 6. - Santa Monica, CA, USA : Human Factors and Ergonomics Society. - 2169-5067. - 9781605606859 ; , s. 518-522
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Conference paper (peer-reviewed)abstract
- We report on two studies of the mere exposure effect on the occurrence of flow. Findings reveal that: (a) pleasurable human-system experience increased linearly with repeated exposure to the technology of interest; (b) an habituation effect of flow was mediated by day; (c) performance was positively correlated to flow. Suggestions for future research directions for Hedonomics include mitigating the habituation of flow effect by incorporating an adaptive hedonomic design to reduce the effect of boredom that comes with familiar stimuli an approach that enables the user to create a balance between typicality and novelty in order to allow for changing cultural norms and personal change over time.
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| 7. |
- Vorobyeva, Anzhelika, et al.
(author)
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Indirect Radioiodination of DARPin G3 Using N-succinimidyl-Para-Iodobenzoate Improves the Contrast of HER2 Molecular Imaging
- 2019
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In: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 20:12
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Journal article (peer-reviewed)abstract
- Radionuclide molecular imaging of human epidermal growth factor receptor 2 (HER2) in breast and gastroesophageal cancer might be used to stratify patients for HER2-targeted therapy as well as monitor treatment response and disease progression. Designed ankyrin repeat proteins (DARPins) are small engineered scaffold proteins with favorable properties for molecular imaging. Herein we compared two methods for labeling the anti-HER2 DARPin (HE)(3)-G3, direct and indirect radioiodination. We hypothesized that the use of N-succinimidyl-para-iodobenzoate (SPIB) for radioiodination would facilitate the clearance of radiometabolites and improve the contrast of imaging. Both radiolabeled (HE)(3)-G3 variants preserved their binding specificity and high affinity to HER2-expressing cells. The specificity of tumor targeting in vivo was also demonstrated. A biodistribution comparison of [I-125]I-(HE)(3)-G3 and [I-125]I-PIB-(HE)(3)-G3, in mice bearing HER2 expressing SKOV3 xenografts, showed rapid clearance of [I-125]I-PIB-(HE)(3)-G3 from normal organs and tissues and low accumulation of activity in organs with NaI-symporter expression. Both radiolabeled (HE)(3)-G3 variants had equal tumor uptake. Consequently, the indirect label provided higher tumor-to-blood and tumor-to-organ ratios compared with the direct label. Comparative Single Photon Emission Computed Tomography (SPECT)/CT imaging of HER2 expression in SKOV3 xenografts, using both radiolabeled DARPins, demonstrated the superior imaging contrast of the indirect label. Indirect radioiodination of (HE)(3)-G3 using SPIB could be further applied for SPECT and PET imaging with iodine-123 and iodine-124.
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| 10. |
- Kasraei, Ahmad, et al.
(author)
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Climate Zone Reliability Analysis of Railway Assets
- 2024
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In: International Congress and Workshop on Industrial AI and eMaintenance 2023. - : Springer Science and Business Media Deutschland GmbH.
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Conference paper (other academic/artistic)
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