| 1. |
- Chen, S, et al.
(författare)
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Functional association of cytokine-induced SH2 protein and protein kinase C in activated T cells
- 2003
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Ingår i: International Immunology. - : Oxford University Press (OUP). - 1460-2377. ; 15:3, s. 403-409
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Tidskriftsartikel (refereegranskat)abstract
- TCR signaling is mediated by intracellular signaling molecules and nuclear transcription factors, which are tightly regulated by interaction with regulatory proteins such as Grb2 and SLAP. We reported recently that TCR stimulation induces the expression of cytokine-induced SH2 protein (CIS). The expression of CIS promotes TCR-mediated activation. We have now found specific interactions between CIS and activated protein kinase C (PKC) alpha, beta and theta in TCR-stimulated T cells. CIS was shown by in vitro kinase assay to associate with activated PKC. In CIS-expressing T cells isolated from CIS-transgenic mice, the amount of activated PKC associated with CIS was found to increase following TCR stimulation. By immunohistochemical analysis, CIS was also found to co-localize with PKCtheta at the plasma membrane of activated T cells. In addition to the interaction and intracellular co-localization of the CIS and PKC, an increase in the activation of AP-1 and NF-kappaB was noted in CIS-expressing T cells, after stimulation by either anti-CD3/CD28 or phorbol myristate acetate + ionomycin. These results suggest that CIS regulates PKC activation, and that this may be important for the activation of both the AP-1 and NF-kappaB pathways in TCR signaling.
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| 2. |
- Fu, Zhan, et al.
(författare)
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Evaluation of the difference between the driving behaviour of a Speech based and a speech-visual based task of an in-car computer
- 2004
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Ingår i: The 8th International Conference on Spoken Language Processing, Interspeech 2004, ICSL. Jeju Island, Korea.
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Konferensbidrag (refereegranskat)abstract
- To select the right modality for the interaction between drivers and the in-vehicle information system (IVIS) is crucial for safety reasons. This paper presents an experimental study to dress on this subject. The study was carried out on a 160 degree car-driving simulation lab. There are 10 subjects participated in the experiment. We compared the subjects driving behavior on speech input/output only and speech input with speech+visual output interaction modalities with a simple IVIS. To judge the safety status of subjects driving performance, two independent variables which includes the average division of over speed and the average division of the car out of lane were measured as dangerous extent. Result indicates that it is not significant differences of driving performance by using synthetic speech to replace the visual display in the IVIS. It indicated that the visual presentation of a multi-modal IVIS can be acts as redundancy or complementary modality for auditory presentation, which will aids in relieving the resource demand.
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| 3. |
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| 4. |
- Paulsson, Kajsa M, et al.
(författare)
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Association of tapasin and COPI provides a mechanism for the retrograde transport of major histocompatibility complex (MHC) class I molecules from the Golgi complex to the endoplasmic reticulum
- 2002
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Ingår i: Journal of Biological Chemistry. - 1083-351X. ; 277:21, s. 18266-18271
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Tidskriftsartikel (refereegranskat)abstract
- Tapasin is a subunit of the transporter associated with antigen processing (TAP). It associates with the major histocompatibility complex (MHC) class I. We show that tapasin interacts with beta- and gamma-subunits of COPI coatomer. COPI retrieves membrane proteins from the Golgi network back to the endoplasmic reticulum (ER). The COPI subunit-associated tapasin also interacts with MHC class I molecules suggesting that tapasin acts as the cargo receptor for packing MHC class I molecules as cargo proteins into COPI-coated vesicles. In tapasin mutant cells, neither TAP nor MHC class I are detected in association with the COPI coatomer. Interestingly, tapasin-associated MHC class I molecules are antigenic peptide-receptive and detected in both the ER and the Golgi. Our data suggest that tapasin is required for the COPI vesicle-mediated retrograde transport of immature MHC class I molecules from the Golgi network to the ER.
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