| 1. |
- Ferretti, Ana B. S. M., et al.
(författare)
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High dynamism for neo-sex chromosomes : satellite DNAs reveal complex evolution in a grasshopper
- 2020
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Ingår i: Heredity. - : NATURE PUBLISHING GROUP. - 0018-067X .- 1365-2540. ; 125:3, s. 124-137
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Tidskriftsartikel (refereegranskat)abstract
- A common characteristic of sex chromosomes is the accumulation of repetitive DNA, which accounts for their diversification and degeneration. In grasshoppers, the X0 sex-determining system in males is considered ancestral. However, in some species, derived variants like neo-XY in males evolved several times independently by Robertsonian translocation. This is the case ofRonderosia bergii, in which further large pericentromeric inversion in the neo-Y also took place, making this species particularly interesting for investigating sex chromosome evolution. Here, we characterized the satellite DNAs (satDNAs) and transposable elements (TEs) of the species to investigate the quantitative differences in repeat composition between male and female genomes putatively associated with sex chromosomes. We found a total of 53 satDNA families and 56 families of TEs. The satDNAs were 13.5% more abundant in males than in females, while TEs were just 1.02% more abundant in females. These results imply differential amplification of satDNAs on neo-Y chromosome and a minor role of TEs in sex chromosome differentiation. We showed highly differentiated neo-XY sex chromosomes owing to major amplification of satDNAs in neo-Y. Furthermore, chromosomal mapping of satDNAs suggests high turnover of neo-sex chromosomes inR. bergiiat the intrapopulation level, caused by multiple paracentric inversions, amplifications, and transpositions. Finally, the species is an example of the action of repetitive DNAs in the generation of variability for sex chromosomes after the suppression of recombination, and helps understand sex chromosome evolution at the intrapopulation level.
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| 2. |
- Palacios-Gimenez, Octavio M., et al.
(författare)
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Eight Million Years of Satellite DNA Evolution in Grasshoppers of the Genus Schistocerca Illuminate the Ins and Outs of the Library Hypothesis
- 2020
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Ingår i: Genome Biology and Evolution. - : Oxford University Press (OUP). - 1759-6653. ; 12:3, s. 88-102
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Tidskriftsartikel (refereegranskat)abstract
- Satellite DNA (satDNA) is an abundant class of tandemly repeated noncoding sequences, showing high rate of change in sequence, abundance, and physical location. However, the mechanisms promoting these changes are still controversial. The library model was put forward to explain the conservation of some satDNAs for long periods, predicting that related species share a common collection of satDNAs, which mostly experience quantitative changes. Here, we tested the library model by analyzing three satDNAs in ten species of Schistocerca grasshoppers. This group represents a valuable material because it diversified during the last 7.9 Myr across the American continent from the African desert locust (Schistocerca gregaria), and this thus illuminates the direction of evolutionary changes. By combining bioinformatic and cytogenetic, we tested whether these three satDNA families found in S. gregaria are also present in nine American species, and whether differential gains and/or losses have occurred in the lineages. We found that the three satDNAs are present in all species but display remarkable interspecies differences in their abundance and sequences while being highly consistent with genus phylogeny. The number of chromosomal loci where satDNA is present was also consistent with phylogeny for two satDNA families but not for the other. Our results suggest eminently chance events for satDNA evolution. Several evolutionary trends clearly imply either massive amplifications or contractions, thus closely fitting the library model prediction that changes are mostly quantitative. Finally, we found that satDNA amplifications or contractions may influence the evolution of monomer consensus sequences and by chance playing a major role in driftlike dynamics.
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| 3. |
- Nasseriyan, Pouriya, et al.
(författare)
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Numerical and Experimental Study of an Asymmetric CPC-PVT Solar Collector
- 2020
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Ingår i: Energies. - : MDPI. - 1996-1073. ; 13:7
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Tidskriftsartikel (refereegranskat)abstract
- Photovoltaic (PV) panels and thermal collectors are commonly known as mature technologies to capture solar energy. The efficiency of PV cells decreases as operating cell temperature increases. Photovoltaic Thermal Collectors (PVT) offer a way to mitigate this performance reduction by coupling solar cells with a thermal absorber that can actively remove the excess heat from the solar cells to the Heat Transfer Fluid (HTF). In order for PVT collectors to effectively counter the negative effects of increased operating cell temperature, it is fundamental to have an adequate heat transfer from the cells to the HTF. This paper analyzes the operating temperature of the cells in a low concentrating PVT solar collector, by means of both experimental and Computational Fluid Dynamics (CFD) simulation results on the Solarus asymmetric Compound Parabolic Concentrator (CPC) PowerCollector (PC). The PC solar collector features a Compound Parabolic Concentrator (CPC) reflector geometry called the Maximum Reflector Concentration (MaReCo) geometry. This collector is suited for applications such as Domestic Hot Water (DHW). An experimental setup was installed in the outdoor testing laboratory at Gävle University (Sweden) with the ability to measure ambient, cell and HTF temperature, flow rate and solar radiation. The experimental results were validated by means of an in-house developed CFD model. Based on the validated model, the effect of collector tilt angle, HTF, insulation (on the back side of the reflector), receiver material and front glass on the collector performance were considered. The impact of tilt angle is more pronounced on the thermal production than the electrical one. Furthermore, the HTF recirculation with an average temperature of 35.1C and 2.2 L/min flow rate showed that the electrical yield can increase by 25%. On the other hand, by using insulation, the thermal yield increases up to 3% when working at a temperature of 23 C above ambient.
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