| 1. |
- Bjelic, Sinisa, et al.
(författare)
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Computational design of enone-binding proteins with catalytic activity for the Morita-Baylis-Hillman reaction
- 2013
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Ingår i: ACS Chemical Biology. - : American Chemical Society (ACS). - 1554-8929 .- 1554-8937. ; 8:4, s. 749-757
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Tidskriftsartikel (refereegranskat)abstract
- The Morita-Baylis-Hillman reaction forms a carbon-carbon bond between the α-carbon of a conjugated carbonyl compound and a carbon electrophile. The reaction mechanism involves Michael addition of a nucleophile catalyst at the carbonyl β-carbon, followed by bond formation with the electrophile and catalyst disassociation to release the product. We used Rosetta to design 48 proteins containing active sites predicted to carry out this mechanism, of which two show catalytic activity by mass spectrometry (MS). Substrate labeling measured by MS and site-directed mutagenesis experiments show that the designed active-site residues are responsible for activity, although rate acceleration over background is modest. To characterize the designed proteins, we developed a fluorescence-based screen for intermediate formation in cell lysates, carried out microsecond molecular dynamics simulations, and solved X-ray crystal structures. These data indicate a partially formed active site and suggest several clear avenues for designing more active catalysts.
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| 2. |
- Hokelek, I., et al.
(författare)
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TUBITAK BILGEM wireless telecommunication technologies research laboratory
- 2013
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Konferensbidrag (refereegranskat)abstract
- In this paper, we provide information about Wireless Telecommunication Technologies Research Laboratory (KITAL) which has been established at TUBITAK BILGEM under the sponsorship of Ministry of Development. KITAL, which will significantly increase the R&D capabilities of Turkey in the area of wireless telecommunication technologies, provides a laboratory infrastructure for experimenting different aspects of heterogenous networks. It will include equipments for R&D activities of both second and third generation technologies (GSM, UMTS, etc.) and fourth generation technologies (LTE, WiMax, etc.) whose standardization has been going on. KITAL will also provide infrastructure for R&D of next generation tactical wireless telecommunication technologies towards enabling Network Centric Warfare. KITAL infrastructure will be available to other organizations to support wireless telecommunication R&D activities in Turkey.
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| 3. |
- Kuyuk, R. T., et al.
(författare)
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An interoperability testbed for VoIP terminals
- 2013
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Konferensbidrag (refereegranskat)abstract
- We present an interoperability test automation system developed by TUBITAK BILGEM for Wireless Telecommunication Technologies Research Lab (KITAL) project under the sponsorship of Ministry of Development. This test system, called Shadow Coordinator, aims to verify the behavior of various wired and wireless terminals developed either at BILGEM or by industrial vendors as defined by SIP, SCIP and other related protocol specifications. Minimum-length and maximum coverage test sequences are generated using EFSM models and techniques defined in test theory. At the first stage of the project, interoperability of MILSEC-4 terminals is targeted.
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| 4. |
- Kuyuk, R. T., et al.
(författare)
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Interoperability of secure VoIP terminals
- 2013
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Konferensbidrag (refereegranskat)abstract
- An interoperability test automation system has been implemented at the Wireless Communication Technologies Research Laboratory (KITAL) at TUBITAK BILGEM for secure IP communication terminals. Designed and manufactured at BILGEM, MILSEC-4 terminals provide VoIP signalling using SIP and end-to-end secure communication using SCIP protocols. This test system, called Shadow Coordinator, aims to verify the interoperability of terminals implementing SCIP, SIP and other related protocols. A simplified EFSM model for the expected behavior of a terminal implementing SCIP is introduced. A more advanced version of this model is used to genearate minimum-length and maximum coverage test sequences.
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