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- Bisagni, Serena, et al.
(författare)
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Cloning and expression of a Baeyer-Villiger monooxygenase oxidizing linear aliphatic ketones from Dietzia sp. D5
- 2014
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Ingår i: Journal of Molecular Catalysis B: Enzymatic. - : Elsevier BV. - 1873-3158 .- 1381-1177. ; 109, s. 161-169
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Tidskriftsartikel (refereegranskat)abstract
- A Baeyer-Villiger monooxygenase has been identified in the genome sequence of Dietzia sp. D5. Sequence similarity search revealed that the enzyme belongs to a group of BVMOs that are closely related to ethionamide monooxygenase from Mycobacterium tuberculosis (EthA). The BVMO was expressed in E. coli BL21-CodonPlus(DE3)-RP and the best expression was achieved when the E. coli cells were cultivated in terrific broth (TB) at 15 degrees C and induced with 0.1 mM of IPTG. Since the purified enzyme did not show any measurable activity, the substrate scope of the BVMO has been determined using whole-cell and crude cell extract systems. The enzyme was most active towards linear aliphatic substrates. However, it has shown a moderate degree of conversion for cyclobutanone, 2-methylcyclohexanone, bicyclo[3.2.0]hept-2-en-6-one, phenylacetone and thioanisole. There was no detectable conversion of ethionamide, cyclohexanone and acetophenone. (C) 2014 Elsevier B.V. All rights reserved.
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| 2. |
- Chávez, Georgina, et al.
(författare)
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Baeyer-Villiger Oxidation of Cyclohexanone in Aqueous Medium with In Situ Generation of Peracid Catalyzed by Perhydrolase CLEA
- 2014
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Ingår i: Topics in Catalysis. - : Springer Science and Business Media LLC. - 1572-9028 .- 1022-5528. ; 57:5, s. 349-355
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Tidskriftsartikel (refereegranskat)abstract
- A perhydrolase, immobilized as a cross linked enzyme aggregate (CLEA), was employed to catalyze the in situ formation of peracetic acid (PAA) from ethylene glycol diacetate (EGDA) and hydrogen peroxide. The produced PAA was used for the Baeyer-Villiger oxidation of cyclohexanone, which afforded caprolactone in 63 % yield. The effect of type and amount of acyl donor, solvent, pH, temperature and ratio of cyclohexanone to hydrogen peroxide on the production of caprolactone was studied. The highest caprolactone yield was obtained with 100 mM EGDA as the acyl donor at pH 6 and room temperature using a ratio of cyclohexanone to hydrogen peroxide ratio of 1:4. Interestingly, the perhydrolase CLEA exhibited the highest activity in aqueous medium in contrast to the well studied lipase B from Candida antarctica. The perhydrolase CLEA proved to be a very efficient catalyst; the K (m) and V-max values were 118 mM and 56.3 mu mol min(-1), respectively.
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